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The third fluroquino is one kind of antibiotics which is often used in ophthalmology. It's antibacterial activity for Gram-negative bacteria is better than that for Gram-positive bacteria and it is the weakest for anaerobe and endo-pathogens.

第三代氟喹诺酮类药物是眼科常用的抗菌药物,它对革兰氏阴性菌有良好的抗菌活性,但对革兰氏阳性菌(如肺炎球菌、化脓性链球菌、肠球菌)的抗菌作用相对较弱,对厌氧菌和胞内病原体(衣原体、支原体、军团菌、分枝杆菌)作用较差。

The third fluroquino is one kind of antibiotics which is often used in ophthalmology. Its antibacterial activity for Gram-negative bacteria is better than that for Gram-positive bacteria and it is the weakest for anaerobe and endo-pathogens.

第三代氟喹诺酮类药物是眼科常用的抗菌药物,它对革兰氏阴性菌有良好的抗菌活性,但对革兰氏阳性菌(如肺炎球菌、化脓性链球菌、肠球菌)的抗菌作用相对较弱,对厌氧菌和胞内病原体(衣原体、支原体、军团菌、分枝杆菌)作用较差。

The radical scavenging activity of Laminaria japonica pigments was studied by the DPPH2,2-Diphenyl~(-1-picrylhydrazylassay. The total pigments showed a strong DPPH radical scavenging activity of 65.819% with the concentration of 0.52mg.ml~(-1). The DPPH radical scavenging activity of fucoxanthin was 19.85% with the concentration of 0.27mg.ml~(-1). The antimicrobial activity of pigment in Laminaria japonica was studied by the method of round paper. The results showed that the pigment extracts have activity against Escherichia coli, Pseudomonas aeruginosa, Saricina flava and Saccharomyes cerevisia. The antibacterial activity of total pigments for Saccharomyes cerevisia was the strongest with an inhibition diameter of 18.9 mm; and the antibacterial activity of fucoxanthin for Saricina flava was also much significant with an inhibition diameter of 10.2 mm.

测定了它们对自由基的清除率和对四种细菌的抑菌效果,研究结果表明,总色素和褐藻黄素的浓度与自由基清除率成正比,当总色素浓度为0.52mg.ml~(-1)时,清除率达到65.819%,高于陆生植物黑米色素和紫甘薯色素;当褐藻黄素浓度为0.27mg.ml~(-1)时,自由基清除率达到19.85%,低于紫苏色素、紫甘蓝色素、黑米色素、紫甘薯色素;对大肠杆菌、绿脓杆菌、八叠球菌、金黄葡萄球菌的抑菌实验结果显示,海带总色素对金黄葡萄球菌的抑菌活性最强,抑菌圈直径为18.9mm;褐藻黄素对八叠球菌的抑菌活性相对较强,抑菌圈直径为10.2mm。

Methods From Feb 2006 to Jun 2006,188 hospitalized children in Shenzhen children s hospital, were collected deep tracheal aspirate at the time of hospitalization. The respiratory tract secretions were immediately sent for bacterial culture with 3 kinds of medium:ordinary medium, Hemophilus influenzae selective medium, Streptococcus penumoniae selective medium. Then we extracted the total nucleic acids from secretions, and detected Mycoplasma pneumoniae by single fluorescent quantitation PCR. Simultaneously, 14 respiratory tract pathogenic bacterium and Mycoplasma pneumoniae were detected by Target Enriched multiplex PCR. Amplification products were identified by the Luminex100 suspension array.

确诊为社区获得性肺炎的患儿188例,在入院当天采集深部呼吸道吸引物,用普通培养基和肺炎链球菌、流感嗜血杆菌选择性培养基进行细菌培养,然后提取深部呼吸道吸引物中病原体的DNA,采用荧光定量单PCR的方法检测肺炎支原体,并对同一标本采用靶序列富集多重PCR技术同时扩增肺炎链球菌、流感嗜血杆菌、金黄色葡萄球菌、肺炎克雷伯菌、大肠杆菌、嗜肺军团菌、铜绿假单胞菌、鲍曼氏不动杆菌、脑膜炎奈瑟氏菌、阴沟肠杆菌、奇异变形杆菌、化脓链球菌、粪肠球菌及屎肠球菌14种呼吸道病原菌和肺炎支原体的靶基因,扩增产物用Luminex100多功能悬浮点阵仪检测。

Lactic acid bacteria are widely distributed in the nature. The genus Lactobacillus, Lactococcus, Pediococcus and Leuconostoc are included being representatives in this group.

乳酸菌在自然界分布广泛,其中比较有代表性的菌属为乳杆菌属、乳球菌属、片球菌属和明串珠球菌属。

This study has discussed that Lactic acid bacteria are isolated from 752 samples of milk and other dairy products, collected from 500 household in pastoral areas in Inner mongolia, and its biological properties are analyzed and studied.765 strains of lactic acid bacteria, including 28 species of Lactobacellus,2 species strains of Bifidobacterium, 9 species strains of Enterococcus, 3 species strains of Lactococcus, 1 specie of Streptococcus, 6 species of Leuconostoc,1 specie of Pediococcus , are identified.

从代表内蒙古牧区不同地域特点和独特生态环境的9个盟市18个牧业旗500多户牧民家庭,以目的采样和层次分类随机采样相结合的方法,共采集鲜乳及乳制品样品752份,分离到947株乳酸菌,并用形态、染色、培养特性及生化反应等实验对其进行了鉴定,有765株实验菌株分别归于7个属50个种和亚种,其中归于乳杆菌属的有28个种和亚种;归于双歧杆菌属的有2个种;归于肠球菌属的有9个种;归于链球菌属的有1个种;归于乳球菌属的有3个种和亚种;归于明串珠菌属的有6个种和亚种;归于片球菌属的有1个种。

Anaerobicbacteria harmful to beer includes lactobacillus, tetracoccus, pectic bacillus and macrococcus, among which lactobacillus and tetracoccus are the commonest which change beer flavour and taste.

对啤酒有害的厌氧菌有乳酸杆菌、四联球菌、果胶杆菌、巨球菌,其中乳酸杆菌与四联球菌是最常见的啤酒有害菌,可使啤酒产生风味和口味上的败坏。

Anaerobicbacteria harmful to beer includes lactobacillus, tetracoccus, pectic bacillus and macrococcus, among which lactobacillus and tetracoccus are the commonest which change beer flavour and taste.

摘 要:对啤酒有害的厌氧菌有乳酸杆菌、四联球菌、果胶杆菌、巨球菌,其中乳酸杆菌与四联球菌是最常见的啤酒有害菌,可使啤酒产生风味和口味上的败坏。

Results totally 287 strains were isolated from the 256 positive samples , and the gram-negative bacilli were 225(78.4%,), the gram-positive coccus were 41(14.3%), snd the monilia were 21(7.3%).the distributions of clinical bacteria were respiratory tract(63.4%),urinaryract(7.0%),secretion(includingwound .3%),blood(5.9%),stool(5.2%), pucture fluid(4.9%), and other sites(7.3%). of all isolating bacterium,from the first to the fifth were ps.aeruginosa(19.5%),k.pneumoniae(16.7%), e.coli(14.3%), a.baumannii(11.8%) and psemal (10.1%).resistant rates of methecillin-resistant s.aureus,methecillin-resistant coagulase-negative staphylococci and vancomycin-resistantwere 88.2%、70.0% and 11.1% respectively;the incidence of e.coli and k.penumoniae produce extended speutrum beta-lactamase were 68.6%和65.2%, 44.6% of ps.aeruginosa isolates were resistant to imipenem; the highest examining rate of 21 kinds of monilia was candida albicans (66.7%),resistant rate of candida albicans to fluconazole and amphotricin b was 51.3% and 1.3%.

结果 在254份检出细菌阳性标本中共培养出287株细菌,其中革兰阴性杆菌225株(78.4%),革兰阳性球菌41株(14.3%),念珠菌21株(7.3%),检出菌来自呼吸道标本占63.4%,其他标本各占5%左右;细菌检出占构成比前三位的依次为铜绿假单胞菌19.5%、肺炎克雷伯菌16.7%、大肠埃希菌14.3%;耐甲氧西林金黄色葡萄球菌、耐甲氧西林凝固酶阴性葡萄球菌和耐万古霉素的肠球菌的发生率分别为88.2%、70.0%和11.1%;大肠埃希菌和肺炎克雷伯菌的超广谱β-内酰胺酶的检出率分别为68.6%和65.2%,铜绿假单胞菌对亚胺培南的耐药率为40.2%;白色念珠菌对氟康唑的耐药率为81.3%,对两性霉素的耐药率为3.2%。

And the transformation efficiency of Enterococcus faecalis is obviously higher than that of Lactobacillus plantarium by pulse electroporation with different voltages, which suggests that the two strains of LAB have different usage in the research of genetic engineering of LAB.

经过16SRNA全长序列分析及配合生理生化实验鉴定,结果显示球菌为肠球菌属中的粪肠球菌,杆菌为乳酸杆菌属中的植物乳杆菌,是两株胃肠道内正常定植菌。

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