球菌

All results from morphological, physiological and biochemical characterization, the 16S rDNA sequence and phylogenetic assay identified that the strain belongs to Micrococcus luteus. The DNA fragment encoding mature peptide of fibrinolytic enzyme was cloned by PCR and sequenced.

利用传统细菌分类鉴定方法对其形态和生理生化特征进行了研究，发现其与藤黄微球菌的特征相符， 16S rDNA序列分析的结果也表明它与藤黄微球菌的16S rDNA序列的同源性高达99%，因此该菌在系统分类学上属于微球菌属藤黄微球菌。

Compared with the Giant panda in captivity, the predominantintestinal florae of Giant panda returned to wild were still enterobacteria(Escherichia coli, Enterobacter aerogenes, Proteus, Salmonella, Yersinia enterocolitica), enterococcus and lactobacillus after Giant Panda had been returned to wild for a week.

与圈养大熊猫相比，放归大熊猫在放归一周后，肠道正常菌群中优势菌群仍为肠杆菌(大肠杆菌、产气肠杆菌、变形杆菌、沙门氏菌、小肠结肠炎耶尔森氏菌)，肠球菌和乳杆菌，但细菌数量即开始发生了一定变化，表现在肠球菌数量明显增加，乳杆菌数量明显减少，肠杆菌数量有轻微减少，到采样后期，肠球菌数量超过肠杆菌；另外，放归大熊猫粪样中芽抱杆菌和酵母菌的检出率较高，分别为60%和40%。

P 0.05. Total ruminal bacteria, proteolytic and amylolytic bacteria, and protozoa in rumen enumerated by culture method all increased (P 0.05) in the Trial period. Real-time PCR analysis was used to compare Pre-trial and Trial populations of cellulolytic species, including Butyrivibrio fibrisolvens, Ruminococcus flavefaciens and Ruminococcus albus, which were increased by 7.0, 4.5 and 9.6%(P 0.05), respectively. Two starch-fermenting bacteria species, Streptococcus bovis and Ruminobacter amylophilus, were increased by 25.4% and 14.6%(P 0.05), respectively.

3.02，P 0.05。传统培养法结果表明，与对照期相比，BSN2处理期奶牛瘤胃中瘤胃细菌、蛋白分解菌、淀粉分解菌和原虫数量显著增加；实时定量PCR结果表明，试验期奶牛瘤胃纤维分解菌，包括溶纤维丁酸弧菌、黄色瘤胃球菌和白色瘤胃球菌数量分别提高6.97%、4.51%和9.6%(P 0.05)，两种淀粉分解菌，牛链球菌和嗜淀粉瘤胃杆菌分别提高25.4%和14.6%(P 0.05)，乳酸利用菌埃氏巨球型菌也提高8.85%(P 0.05)。

Fluorescent instruments.the colonies of the specimens yielding growth were identified by the vitek32 instruments and kb method was used for the drug sensitivity tests.results three hundred and thirtyone bacteria strains were isolated from a total of 3104 blood culture specimens,with a positive rate of 10.7%.of the identified bacteria,g+ cocci accounted for 50.3%,g-bacilli accounted for 44.7%,fungi accounted for 3.0%,and anaerobian accounted for 1.1%.the susceptibility rates of g-bacilli to imipenem,amikacin and cefoperazone/sulbactam were high.the susceptibility rate of g+ cocci to vancomycin and imipenem were also high.conclusion g+ cocci prevail over g-bacilli in the blood specimens.the staphylococci are the chief bacteria in the childrens blood specimens.both g+ cocci and g-bacilli are sensitive to imipenem.

结果 在3 104份血液培养标本中分离出病原菌331株，阳性检出率为10.7%。病原菌以革兰阳性需氧球菌居首位（50.3%），革兰阴性需氧杆菌次之（44.7%），真菌占3.0%，厌氧菌占1.1%。血液培养中的g+球菌对万古霉素和亚胺培南较为敏感，g-杆菌对亚胺培南、舒普深、丁胺卡那较为敏感。结论血液培养病原菌以g+球菌为主，g-杆菌次之；儿童血液培养病原菌以葡萄球菌属为主；亚胺培南对g+球菌和g-杆菌均具有较高的敏感率。

Staphylococcus auricularis, Staphylococcus xylosus and Pediococcus pentosaceus had a signifient effect to the sensory quality of dried beef, Pediococcus. pentosaceu had a most signifiennt effect to improve dried beefs quality. According to all the factors, Staphylococcus xylosus, Lactobacillus plantarum and Pediococcus pentosaceus were proved to be appropriate.

结果表明：各菌株对发酵牛肉成品的pH、酸度、氨基酸总量的影响未达到显著水平；植物乳杆菌、戊糖片球菌具有增加产品中游离脂肪酸含量作用；松鼠葡萄球菌、耳氏葡萄球菌、枯草芽孢杆菌、木糖葡萄球菌、腐生葡萄球菌和戊糖片球菌则具有显著增加不同游离氨基酸的作用；从感官评价结果来看，耳氏葡萄球菌、木糖葡萄球菌、戊糖片球菌能显著影响产品的感官特性，其中戊糖片球菌能显著提高产品感官评价分值。

Three hundred and fifty-nine strains of bacterium and fifty-seven strains of yeast isolated from foregut, hindgut, coelomic liquid and body surface of Stichopus japonicus were studied for biochemical activities.

从刺参Stichopus japonicus前后消化管、体腔液和表皮上所分离到的359株细菌分别属于弧菌属、假单胞菌属、奈瑟氏球菌属、不动杆菌属、柄杆菌属、黄杆菌属、节杆菌属、微球菌属、黄单胞菌属、棒杆菌属、产碱菌属这十一个主要菌属；57株酵母菌分别属于球拟酵母属、红酵母属、隐球酵母属和德巴利氏酵母属四个属。

The results showed that at the anaerobic phase, the PHB concentration in the cell and orthophosphate concentration of the anaerobic effluent obviously increased, the poly-P concentration in the cell decreased, and the cucci and bacilli with a size of 1.0~1.5 μm were dominant and formed grapy cellular cluster. At the anoxic phase, the PHB concentration and orthophosphate concentration decreased significantly, the amount of poly-P increased, and the elliptical cucci of 0.5~1 μm and spheral bacilli of 1.0~1.5 μm were dominant. At the aerobic phase, the PHB and orthophosphate concentration almost reduced to zero, the poly-P increased significantly, cucci appeared single or in pair, and the contour of sphere was blur and almost disappeared.

试验结果表明，在厌氧阶段，微生物细胞内PHB的量大幅提高，上清液中磷酸盐的含量上升，聚磷酸盐含量明显下降，粒径为1～1.5 μm、呈球状和棒状的菌群构成的葡萄状细胞簇占居优势；在缺氧阶段，微生物细胞内PHB的量下降，上清液中磷酸盐含量下降，聚磷酸盐含量上升，粒径为0.5～1 μm的椭球菌与1.0～1.5 μm的球杆菌占优势；在好氧阶段，微生物细胞内PHB的量较低或接近零，上清液中磷酸盐的含量接近零，聚磷酸盐含量明显上升，此时粒径1.0～1.5 μm的球菌以单个或成对出现，球菌不再饱满，呈现接近消失的状态。

From 94 soil samples and 9 bark samples in these areas, 259 Myxobacteria strains were isolated and 140 strains of them were purified. These 259 Myxobacteria strains were primarily identified in genus or species level by their fruiting bodies, myxospores and vegetative cells on the base of Sergey's manual of determinative bacteriology and they belonged to 9 genera(Myxococcus, Corallococcm. Angiococcus. Archangium, Cystobacter. Stigmatella . Hapoangium. Nannocystis. Polyangium) of Myxococcales.

根据菌株的特征性子实体形态和其营养细胞、粘孢子形态，以《Bergey's manualof determinative bacteriology》第九版为依据，将分离纯化出的菌株初步鉴定到属，这259株粘细菌菌株经过初步的分类鉴定，分属于9个属，即粘球菌属139株、珊瑚球菌属20株、囊球菌属18株、原囊菌属21株、孢囊杆菌属5株、标桩菌属36株、单囊菌属9株、小囊菌属3株，和多囊菌属8株。

Results In the condition of obturation and humidity, Neisseria gonorrhoeae in the leucorrheas survived longer in middle quantity than in few and mass. The survival time of Neisseria gonorrhoeae was short in dryness. In a few quantity (10/ml), karyote could phagocytize Neisseria gonorrhoeae and shorten its survival time, but in the quantity above 300/ml, karyote could prolong its survival time.

结果 在密闭保湿状况下，白带标本中的淋球菌在中量时存活时间长，少量或大量时其存活力相对较低；在干燥状况下，淋球菌存活时间短；在菌量很少(10/ml)时，有核细胞可以吞噬淋球菌而导致淋球菌存活时间减少，但在菌量较多时（大于300/ml）时，有核细胞可以延长淋球菌存活时间。

It was found that 0.25%o potassium sorbate produced a positive inhibition against short G+ spore bacillus with a concentration less than 5xl04cfu/ml. A concentration less than %o potassium sorbate hardly exerted a complete control towards short G- plump bacillus having a population density of 5x104cfu/ml. It was proved that use of l% potassium sorbute never controlled the growth of G+ coccus, G~ spirilla and enterobacter with a population density of 104cfu/ml. 1mmol EDTA completely controlled the growth of G+ short spore bacillus and G+ coccus whose cell density was 5xl04cfu/ml. A level of lmmol EDTA showed a limited inhibition against the growth of G- spirilla with a population density of 105 cfu/ml. However, a level of 10mmol EDTA completely controlled the growth of the G- spiral bacteria having a population density of 105cfu/ml. lOmmol EDTA produced a very significant control towards the growth of G"" plump short bacillus with 105cfu/ml. 20mmol EDTA showed a remarkable inhibition against the enterobacter with a population density of 105cfu/ml. Different concentrations of nisin including 25mg/mL, 50mg/mL, 75mg/mL and 100mg/mL were used as bio-preservative to examine its effects against the growth of all strains leading to the spoilage of fresh mutton meat. It was seen that there was a big difference in nisin's concentrations in inhibiting the spoiling bacteria. Generally speaking, as more as 75mg/mL of nisin significantly inhibited the growth of G+ short spore bacillus, G-plump short bacillus, enterobacter, G'spiral bacteria and G+ coccus having a population density of 105cfu/ml.

分别运用山梨酸钾、EDTA和Nisin对7种主要引起羊肉腐败的微生物进行了抑菌实验，结果显示，0.25‰以上山梨酸钾能够有效抑制5×10~4 cfu／mL以下的革兰氏阳性短芽孢杆菌的生长；1‰以下的山梨酸钾不能完全抑制5×10~4 cfu／mL革兰氏阴性粗短杆菌的生长，对10~4 cfu／mL革兰氏阳性球菌菌株、革兰氏阴性螺旋菌菌株和肠杆菌菌株抑制效果不太明显。1mmoL EDTA能完全抑制住小于10~5 cfu／mL革兰氏阳性短芽孢杆菌菌株、革兰氏阳性球菌菌株的生长，能明显的抑制10~5 cfu／mL的革兰氏阴性粗短杆菌菌株生长，对10~5 cfu／mL的革兰氏阴性螺旋菌菌株有一定的抑制作用。10mmoL EDTA能完全抑制住10~5 cfu／mL革兰氏阴性螺旋菌菌株的生长；能明显抑制10~5 cfu／mL的革兰氏阴性粗短杆菌菌株的生长，而20 mmoL EDTA能很明显抑制10~5 cfu／mL肠杆菌菌株的生长。25mg／mL、50 mg／mL、75 mg／mL和100 mg／mL的Nisin几乎对所有引起羊肉的腐败菌有抑制作用，但抑制程度不同，抑菌活性有一定的变化。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。