球外的

The book introduces the animal models representing natural infections ranging from uni- to multicellular and from endo- to ectoparasites in fish, chickens and mammals, including structural and physiological aspects of cell death; Role of apoptosis in non-mammalian host-parasite relationships; thionine-positive cells in relation to parasites; animal models for the study of innate immunity; interaction between sea lice and their hosts; pathophysiology of salmonids cryptobiosis and glossina-transmitted mammalian trypanosomiasis in livestock; avian coccidiosis; interactions betweem fish hosts and monogeneans.

该书介绍了鱼类，小鸡和哺乳动物从单细胞到多细胞和从内寄生虫到外寄生虫的天然传染的动物模型，包括细胞死亡的结构和生理学方向；非哺乳动物宿主和寄生虫关系中细胞凋亡的作用；硫堇阳性细胞与寄生虫的关系；用于先天免疫研究的动物模型；海虱与其宿主的相互作用；鲑鱼隐生想象的生理病理学和家畜中舌蝇传播的哺乳动物锥虫病；鸟类球虫病；鱼类宿主与单殖亚纲动物的相互作用。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Rodriguez hasn't stopped hitting, either, batting .360 since that breakout game as the Yankees, comfortably in control of the AL East, play carefree baseball. He rediscovered his smooth footwork in the field, and his hands felt faster at the plate. He began to wait long enough on pitches to drive them hard to centerfield and rightfield, the satisfying confirmation for a righthanded hitter, like a wink from a pretty girl, that life is good.

A-Rod的手感还持续发热，自从那场对上老虎队的比赛后，他的打击率高达。360，而洋基的战绩也继续笑傲美联东区， A-Rod重新找回了他在守备时的顺畅脚法，以及打击时的挥棒速度，他也开始等到最后一刻才出棒然后将球狠狠地捞到中右外野，这对右打者来说是个令人欣慰的现象

Then the numerical simulation is developed with the above dynamic model. The effects of the clearance on the dynamic characteristics are studied in detail. The influence of factors such as clearance size, driving speed, stiffness, coefficient of viscous damping of contact surface, and the gravity are discussed and the quantitative results are given.

利用本文建立的动力学模型对含间隙三球销移动副的曲柄滑块机构进行了动力学数值仿真，研究了运动副间隙对机构的运动和受力状况的影响，考察了间隙大小、曲柄转速、接触刚度、阻尼、外力矩等对机构动态特性的影响规律，给出了定量结果。

No batter in Major League history has hit a grand slam for his 500th home run, but Alex Rodriguez had a chance later in the three-run sixth inning. Rodriguez instead settled for a sacrifice fly to right, finishing the night still stuck on 499 home runs. His last blast came on July 25 at Kansas City.

虽然A-Rod并没有击出他在大联盟生涯的第500发全垒打，但是他在第六局时有一个三分打点的机会，A-Rod打出了右外野的高飞球，今晚仍旧维持在499发全垒打数，他的最后一发全垒打出现在七月25日对皇家队的比赛中。

His first goal was a simple tap-in from a Ryan Giggs cross, but his second was possibly one of the finest he has scored in a United shirt, a delicate chip over Portsmouth's former England goalkeeper David James from 20 yards.

他的第一个进球是接到莱安·吉格斯的传中球后的轻松推射，而第二个进球可能是他身披曼联战袍所打进的最佳进球之一，20码外一个轻巧的吊射，球越过朴茨茅斯门将、前国门大卫·詹姆斯飞入网窝。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。