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Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在囊胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

The decreased rates of coccidium eggs in Group 3( injecting 2 ml slow-released Chinese herbal medicine per rabbit) was slightly higher than that in Group 5(the diet containing 0.0125% Clopidol or 0.003% Diclazuril) and Group 6 (the diet containing 1% Chinese herb powder).

注射缓释中草药防治兔球虫病的试验结果表明,兔粪便中球虫卵囊的减卵率随中草药缓释剂注射剂量的增加而增加,注射中草药缓释剂2.0ml/只的第3组平均减卵率超过了口服药物的第5组(饲料中含0.0125%氯羟吡啶或地克珠利0.003%)和第6组(饲料中含1%中草药散剂),而不用抗球虫药的第4组球虫卵囊比试验前增加了(232.8 72.3)%。

Based on the security,efficacy,cheapness and easy gain three disinfectant drugs(acids, degerming agents,surface active agents)were chosen to killing coccidial oocysts in chicken.

本试验按照安全、有效、经济、来源容易的原则,从对鸡球虫卵囊有一定抑杀作用的三类消毒药物(酸类、消毒剂类、表面活性剂类)中,筛选了 17组不同药物和浓度的搭配组合,设计了 3个试验:抑制鸡球虫新鲜卵囊孢子化试验;抑杀孢子化卵囊试验;新鲜卵囊和孢子化卵囊经药物处理后感染小鸡试验。

From 94 soil samples and 9 bark samples in these areas, 259 Myxobacteria strains were isolated and 140 strains of them were purified. These 259 Myxobacteria strains were primarily identified in genus or species level by their fruiting bodies, myxospores and vegetative cells on the base of Sergey's manual of determinative bacteriology and they belonged to 9 genera(Myxococcus, Corallococcm. Angiococcus. Archangium, Cystobacter. Stigmatella . Hapoangium. Nannocystis. Polyangium) of Myxococcales.

根据菌株的特征性子实体形态和其营养细胞、粘孢子形态,以《Bergey's manualof determinative bacteriology》第九版为依据,将分离纯化出的菌株初步鉴定到属,这259株粘细菌菌株经过初步的分类鉴定,分属于9个属,即粘球菌属139株、珊瑚球菌属20株、囊球菌属18株、原囊菌属21株、孢囊杆菌属5株、标桩菌属36株、单囊菌属9株、小囊菌属3株,和多囊菌属8株。

Of the 12 patients with basal ganglia and/or capsular lesions, one patient had involvement of the caudate nucleus and the adjacent anterior limb of the internal capsule without any other parenchymal abnormality. In the other 11 cases, the posterior limb of the internal capsule alone (n = 4) or together with the external capsule and putamen (n = 3) or with the globus pallidus (n = 4) were affected. In all 11 of these patients, additional MDJ and/or thalamic-hypothalamic lesions were also found.

在12例基底节区和/或内囊受累的患者中,1例患者仅见尾状核及邻近的内囊前肢受累,其余部位均未见病灶;余下11例患者,内囊后肢单独受累4例;内囊后肢、壳核、外囊共同受累3例;内囊后肢同时伴苍白球受累4例;这11例患者除基底节区和/或内囊受累外,均还可见中脑-间脑结合处和/或丘脑-下丘脑区病灶。

Objective To identify the efficiency of percutanous transluminal balloon mitral valvuloplasty on hemodynamics in patients with mitral stenosis with different heart rhythm and mitral valve area.

目的 明确不同心律和瓣口大小条件时二尖瓣球囊成形术的效果及左房压和肺动脉压改善的情况。

The door 1–to-balloon time was 117 minutes (25th and 75th percentiles, 98 and 137 minutes), with 12.2% and 58% of patients achieving a time of 90 and 120 minutes, respectively.

总的通道1到球囊时间为117分钟(25th和75th百分点,98和137分钟),12.2%和58%患者的完成时间分别是90和120分钟。

The transport and door 2–to-balloon times were 29 minutes (25th and 75th percentiles, 25 and 35 minutes) and 35 minutes (25th and 75th percentiles, 32 and 46 minutes), respectively.

输送和通道2到球囊时间分别为29分钟(25th和75th百分点,25和35分钟)和35分钟(25th和75th百分点,32和46分钟)。

The hyperform balloon catheter was introduced into the right common carotid artery through a small window in the right femoral artery. road map of the right cca was drawn in the control group.

所有动物采用经右侧股动脉入路置入hyperform球囊,实验组行右颈总动脉定比扩张,对照组行单纯右颈动脉路图显示,术后以普通饮食喂养。

If this happens, a second balloon procedure or operation may be necessary to repair aortic stenosis.

如果发生了,再次球囊导管介入或手术对于修复主动脉狭窄可能是必要的。

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