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Fluorescence in situ hybridization was performed to detect the existence of HBV DNA in the blastomere by using the biotinylated probe.

废弃的胚胎卵裂球经激光打孔,活检2~4个卵裂球细胞,利用生物素标记的探针,应用荧光原位杂交技术检测HBV感染情况。

Early embryonic cell has the developmental totipotency. Every embryonic blastomere cell can develop to an intact individual separately in a proper condition.

早期胚胎卵裂球细胞具有发育全能性,每个胚胎卵裂球细胞在适宜的条件下都能单独发育为一个完整的个体。

Microbiopsy of pre-embryos with 4 or 8-cells did not alter their viability and further development. The technique of nested PCR on a single blastomere for the detection of cystic fibrosis ΔF508 mutation is reliable.

在小鼠胚胎4 细胞或8细胞阶段实施单个卵裂球显微活检,不影响胚胎的进一步分化以及小鼠出生后正常生长发育;利用显微活检得到的单个卵裂球进行突变基因诊断是可行的。

The decreased rates of coccidium eggs in Group 3( injecting 2 ml slow-released Chinese herbal medicine per rabbit) was slightly higher than that in Group 5(the diet containing 0.0125% Clopidol or 0.003% Diclazuril) and Group 6 (the diet containing 1% Chinese herb powder).

注射缓释中草药防治兔球虫病的试验结果表明,兔粪便中球虫卵囊的减卵率随中草药缓释剂注射剂量的增加而增加,注射中草药缓释剂2.0ml/只的第3组平均减卵率超过了口服药物的第5组(饲料中含0.0125%氯羟吡啶或地克珠利0.003%)和第6组(饲料中含1%中草药散剂),而不用抗球虫药的第4组球虫卵囊比试验前增加了(232.8 72.3)%。

Under light microscope, the egg is an oblated and agglutinated demersal with a narrow perivitelline space; eggs ranged in diameter from 1.95 to 2.38 mm with a mean of 2.18 ± 0.03 mm; their membrane are smooth and they have 5 to 7 cannular keratose egg-filaments; the oil globules are multiple, ranged in diameter from 0.05 to 0.50 mm and in number from 7 to 145 with a mean of 28±2, the position of them migrate during embryonic development; the yolks are ivory-white with no segments, ranged in diameter from 1.00 to 2.30 mm with a mean of 1.90 ± 0.03 mm; the development phases of the embryo are variable. So they were identified as the eggs of one Beloniformes.

在光学显微镜下:卵呈微扁圆形,卵径1.95~2.38mm,粘着沉性;卵黄间隙较窄,卵黄囊呈乳白色,无龟裂;卵膜平滑,在其表面有5~7根细长角质管状卵膜丝;多油球数不定(4~145),油球径0.05~0.50mm,油球在卵黄囊中的位置随不同的发育时期而不断发生变化;胚体处在不同的发育阶段,其特征符合颌针鱼目鱼卵的特征。

Studies on mouse nuclear transfer with compacted morula blastomeres as donors In this study, C57BL/6 mouse morulae blastomeres and Kunming white mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different sucrose concentration on the manipulation time of nuclear transfer, and the in vitro development of reconstructed embryos.

以致密桑椹胚卵裂球为供体克隆小鼠的研究该研究以C57BL/6 小鼠致密桑椹胚卵裂球为供体、昆明白小鼠MⅡ期卵母细胞为受体,研究了不同蔗糖浓度对核移植操作时间及重构胚体外发育的影响。

In the experiments of artificial infection three of four 12-day-old coccidia-free goslings were died after inoculated with mix oocysts of 5×10~4. Pure oocyst...

通过单种卵囊接种试验获得了5种球虫的纯种卵囊并对它们的一些生物学特征进行了观察,为这些球虫的生活史和致病性研究奠定了基础。

Based on data collected at four stations in Daning River in the spring of 2004 and 2005, phytoplankton community structure, vertical distribution, abundance, biomass, C contents, diversity index, richness index and evenness index were studied. The causes of eutrophication and water blooms in Daning River were analysed and control measures were then put forward. The dominant species were Asterionella Formosa, Cryptomonas ovata, Cyclotella caspia, Stephanodiscus neoastraea in the spring of 2004 and those were Pandorina morum, Eudorina elegans, Cyclotella caspia, Cryptomonas ovata in the spring of 2005. Average cell densities, biomass, C contents in the spring of 2004 and 2005 were 171.1×10^5 cells/L, 12.2 mg/L, 1732.2g C/L and 113.1×10^5 cells/L, 10.1 mg/L, 1395.9g C/L, respectively.

报道了大宁河2004年春季和2005年春季浮游藻类的种类组成、垂直分布、数量、生物量以及C含量的调查结果,并应用多样性指数、丰富度指数和均匀性指数对其水质进行了综合评价;对大宁河蓄水后出现的富营养化现象和春季发生&水华&的原因进行了探讨,同时提出了控制水体富营养化的建议。2004年春季,藻类优势种为美丽星杆藻、卵形隐藻、里海小环藻和新星形冠盘藻;2005年春季的优势种为实球藻、空球藻、里海小环藻和卵形隐藻。2004年和2005年的细胞密度、生物量和C含量分别平均为171.1×10^5 cells/L,12.2 mg/L,1732.2g C/L和113.1×10^5 cells/L,10.1 mg/L,

Based on the security,efficacy,cheapness and easy gain three disinfectant drugs(acids, degerming agents,surface active agents)were chosen to killing coccidial oocysts in chicken.

本试验按照安全、有效、经济、来源容易的原则,从对鸡球虫卵囊有一定抑杀作用的三类消毒药物(酸类、消毒剂类、表面活性剂类)中,筛选了 17组不同药物和浓度的搭配组合,设计了 3个试验:抑制鸡球虫新鲜卵囊孢子化试验;抑杀孢子化卵囊试验;新鲜卵囊和孢子化卵囊经药物处理后感染小鸡试验。

The sixth, there were no significantly differences between colchicine groupsand vinblastine groups on the metaphases, in the experiment of prepare mouse4-cell embryo stage single blastomere chromosome specimen by using optimumparameters; and there were significantly differences between colchicine groupsand vinblastine groups on the metaphases (P<0.05), in the experiment of preparemouse 8-cell embryo stage single blastomere chromosome specimen by usingoptimum parameters.The end, the preparation quality of mouse 4-and 8-cell embryo stage singleblastomere specimen were 2.5, 2.7, 2.4, 1.9, respectively.

低渗液选用0.45%氯化钠时,低渗效果优于选用0.5%柠檬酸钠和0.35%氯化钾的。6、在利用最佳参数制备小鼠4-细胞期胚胎单卵裂球染色体标本时,经统计分析,两种阻断剂的作用效果差异不显著;而在制备小鼠8-细胞期胚胎单卵裂球染色体时阻断剂选用长春花碱的效果优于秋水仙素的(P<0.05)。7、秋水仙素组和长春花碱组小鼠4、8-细胞胚胎单卵裂球染色体标本制备质量分别为:2.5、2.7、2.4、1.9。

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I didn't watch TV last night, because it .

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Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

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