玻璃体内的

Results:Tissue engineered artificial tendon materials of vitreous cryopreservation and non-vitreous cryopreservation in two groups were observed using a scanning electron microscope.At the 2nd week,materials presented a reticular formation and there were juvenal tendon cells among materials.

目的：观察分析玻璃化冷冻保存组织工程化人工肌腱动物体内移植后的超微结构变化特征，探索组织工程化人工肌腱体内移植后的生物学反应和肌腱修复效果，为组织工程化人工肌腱的玻璃化冷冻保存和应用提供重要的理论和实验依据。

The quasi linear dependence of Raman shift upon fiber strain was first used to measure the residual thermal strain along the fiber axis.Then the same dependence was used to determine the fiber strain during pull-out test.Finally the distribution of striain along the fiber axis was converted to the distribution of interfacial shear stress.

利用纤维拉曼峰波数偏移与纤维所受应变的近似线性关系，首先测定纤维热残余应变的分布，随后对伸出玻璃基体外的纤维段施加给定的拉伸应变，沿纤维轴向用拉曼光谱术逐点测定基体内外纤维的应变，最后依据力平衡原理将应变分布转换为界面剪切应力沿纤维轴向的分布。

In the experiment,glass chest was used to simulate indoor roomage environment.The formaldehyde concentration in the simulative room was measured by the method of acetyl acetone with spectrophotometer.

实验以玻璃箱模拟室内空间环境，应用乙酰丙酮分光光度法和邻苯三酚自氧化法测定在不同甲醛含量下芦荟对甲醛的吸收及其体内过氧化物酶活性的变化情况。

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此，本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs，使用无血清培养基进行扩增、培养，用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定，对少突胶质系细胞表达部分营养因子的情况进行检测；采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测；将OPCs移植入成年SD大鼠玻璃体内，利用上丘逆行荧光标记技术，观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间；将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内，观察不同时期视网膜内髓鞘形成与分布特点，分析髓鞘的超微结构，并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

Studies of molecular genetics have demonstrated that retinoblastoma susceptibility gene is closely related to the development of retinoblastoma and the theory of Rb gene mutation or inactivation has been putatively proven to be the molecular mechanism of RB formation.

本研究以我国培养建立的一株RB细胞系〓及裸鼠为实验材料和对象，在对〓细胞抑癌基因（Rb基因、p53基因）表达状况进行检测和鉴定的基础上，通过建立裸鼠玻璃体腔RB常位异种移植模型，构建Rb基因的逆转录病毒表达载体，以脂质体介导的Rb基因转移方式，进行RB体内基因治疗的实验研究，观察Rb基因在体内对RB的治疗作用及影响因素，并探讨其作用机制。

This one contained a large glass vat in which a massi ve, chalk-white, man-shape body floated in clear liquid.

一个巨型的大玻璃桶液体内，清楚地漂浮着一个人形。

When change cross fall and stroke still might maintain the surface of bed movement balance,the spring laid aside in box in vivo,the knot constructs compact,also can time obtain the final ore concentrate and the final debris

在改变横向情侣装厂家直销和冲程时仍可保持床面运行平衡，弹簧重庆玻纤厂在箱体内，结构紧凑，且能有关新型玻璃的作文得出最终精矿和最终尾矿。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。