现有价格

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

The dissertation gets the critical condiction of the media's choices of price modes and the transaction modes with the content providers.

1第2章分析了传媒产业中媒体企业现有的价格模式，构建了双边纵向产品差异的垄断模型。

If that doesn't lead to sustained consumption, commodities prices will fade -- and Rio's shares likely will follow, making any equity issue more dilutive.

如果这无法导致中国大宗商品消费量的持续增长，这类商品的市场价格将会下降，力拓的股价预计也会随之走低，而这将使增资扩股对现有股东权益产生更大稀释作用。

BMD at phalangeal bone measured by RA method is inaccording with osteoprosis epidemiological regular pattern.

且辐射剂量低，误差小，价格低，医院可利用现有X线设备，无须大的投入，即能帮助临床医师准确诊断骨质疏松、确定治疗方案，避免患者的骨密度进一步丢失，减少骨折发生儿率，这是适合边远山区应用的一种骨密度测量方法。

The large number of responses indicates that the Sartorian clothing industry is at risk.

根据调查结果，服装生产商必须设法降低价格来保持他们现有的消费者并维持他们的业务。

The large number o f responses indicates that the Sartorian clothing industry is at risk.

根据调查结果，服装生产商必须设法降低价格来保持他们现有的消费者并维持他们的业务。

An MOF official said:"Frankly, I don't think we can afford further tax cut in 2009 given our tight budget", thus experts argue that, local government may come up with tax cuts, not central government. 2 Shanghai is considering rebating paid personal income tax, and giving out Shanghai citizenship to non-local buyers. 3 Shenzhen: MOLR -local's yesterday guidance: to prevent big fluctuation in the 2009 market.

一位商务部官员表示：&坦白讲，按照现有的紧张预算，2009年再进一步减税完全无法承受&，对此专家表示，地方政府可能实行减税措施，中央政府不会了。2）上海正在考虑向购房者返还已上缴个税，同时将向非上海户籍购房者分配一定的上海市户口。3）深圳：市国土资源局昨日发布通知：2009年房市避免价格波动。

In the narrative that makes up our nine-month journey, we encounter the broken and bleeding body of the American black dozens of times: crossing the Atlantic, sweating in the southern fields, hanging from the lynching tree, hiding from Jim Crow, dodging night-riders and sheriffs, urban sharecropping in ghettos, rioting after their leaders are shot dead, existing within a system of structural violence, paying never-ending rent to a landlord who keeps jacking up the price and never fixing the leaks in the ceiling.

在叙述，使我们的9个月的旅程，我们遇到的破碎和出血机构的美国黑人数十倍：跨越大西洋，出汗的南部油田，挂私刑树，躲避吉姆克劳，逃避夜晚搭车和县行政司法长官，城市佃农在贫民区，骚乱后，其领导人是枪杀，现有的系统结构暴力，支付永无止境的租金的房东让谁顶的价格，决不泄漏固定在天花板上。

Its leading indicators such as connect performance and price are equal to the ones of urea aldehyde resin glue that uses extensively at present. It can meet well the existing wood-based plate production technology.

且胶接性能、价格等主要指标与目前广泛使用的脲醛树脂胶相当，可很好地适应现有的人造板生产工艺。

An all-water anti-freezing system for a solar biogas digester in a severe cold area adopts water as a heat exchange medium to solve the problems of prior anti-freezing system for the solar biogas digester such as high cost, poor heat exchange effect, high energy consumption, and inconvenience in generalization in the rural areas.

严寒地区太阳能沼气池全水防冻系统，它是一种用水作为换热介质的太阳能沼气池防冻系统，以解决现有太阳能沼气池的防冻系统存在的价格昂贵、换热效果差、能耗高、不利于在农村地区推广的问题。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。