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Subsequently, on the basis of the system, the vectors pBar-1C and pBar-1Ab were transformed. 156 and 115 of resistant calli were obtained from 5400 and 4800 calli, respectively. Their transformation efficiency were 2.8% and 2.4%, and differentiation rates were 80.7% and 87.8%, respectively. These results suggest the transformation system is stable as well as efficiency, and good for transformation of rice with Bar gene as selective marker.

随后,以此体系为基础,将装有不同基因的两个载体pBar-1C和pBar-1Ab进行了转化,分别从5400块和4800块愈伤组织中获得156个和115个抗性愈伤,其抗性愈伤分别为2.8%和2.4%,分化分别为80.7%和87.8%,由此说明,此转化体系相对稳定,且分化高、抗性愈伤也较高,可用于同类选择标记的遗传转化,加快水稻转基因育种步伐。

The fusion rate and blastocyst rate of intra-species SCNT embryos was 78.67 (557/708); 56.29%(264/469), that of sub-species or inter-species SCNT embryos were: boer goat 78.18%(541/692); 33.90%(40/118), bovine 70.53%(146/207); 22.52%(25/111), tahr 53.51%(61/114); 5.26%(3/570), panda 79.82%(1159/1452); 8.35%(75/898) and human 68.76%(317/461); 5.41%(16/296), respectively.

种内体细胞核移植胚的融合和囊胚发育分别为78.67%(557/708)和56.29%(264/469);亚种间或种间体细胞核移植胚的融合和囊胚发育分别为:波尔山羊78.18%(541/692)、33.90%(40/118),牛70.53%(146/207)、22.52%(25/111),塔尔羊53.51%(61/114)、5.26%(3/570),熊猫79.82%(1159/1452)、8.35%(75/898),人68.76%(317/461)、5.41%(16/296)。

There was big difference among the 22 different materials in the disease resistance. Translocation lines Pm97033 showed the highest resistance in reaction than others. The percentage of diseased root and severed level were 11.3% and 5.4%; substitution lines Wan7107 showed medium resistance to the fungus, the percentage of diseased root and severity level were 21.4% and 10.6%; F5 generation of common wheat with Till showed higher resistance to take-all fungus than the PS progenies of common wheat with Aegilops tauschii or the other common wheat varieties.

菌饼+菌粒法对宁春4号的致病力明显高于菌饼法和菌粒法,其病根、严重度和病茎分别达到100.0%,57.3%和28.6%。22个遗传背景不同的小麦材料间抗病性差异较大,其中小麦-簇毛麦易位系Pm97033的抗病性最强,病根和严重度分别为11.3%和5.4%;代换系Wan7107次之,病根和严重度分别为21.4%和10.6%;硬粒小麦-簇毛麦双二倍体(TH1)与普通小麦品种杂交后代的抗病性强于粗山羊草与普通小麦品种杂交后代。

A thirty-second rest period was allowed between each testing speed to reduce the effect of fatigue. Results. The SBP (85 to 172 mmHg), HR (65 to 122 beat/min), SpO23.3 to 13.7mL/(kgmin and R (0.72 to 1.04) values during concentric/eccentric exercise were all within the safe range.

结果 男女性别在股四头肌等速向心/离心运动,心跳、血压、每分钟换气量、摄氧量、心跳而压乘积、脉搏血氧饱和度及气体交换未有显著差异:收缩压、舒张压、心跳、摄氧量、每分钟换气量、心跳血压乘绩、脉搏血氧饱和度和气体交换等皆在安全范围。

The reported incidence of non-polio AFP cases was 1.31/lakh among children under 15 years old, the investigating rate of AFP cases within 48 hours of report 95.40%.

15岁儿童非脊髓灰质炎AFP病例报告发病为1.31/10万,报告后48h内调查95.40%,合格粪便标本采集为80.00%,粪便标本7d内及时送检86.90%,随访表75d及时送达为83.80%。

Result: Coefficient of Unguent of water-extraction and alcohol-precipitation method was 17. 2%, Coefficient of Unguent of chitosans clarification method was 12.8%, and macro-reticular absorbing resin method was 3.1%. They could clarify liquor of water-extraction.

结果:乙醇沉淀法所得的浸膏得为17.2%,壳聚糖澄清法所得的浸膏得为12.8%,大孔吸附树脂法所得的浸膏得为3.1%,3种方法均能够较好地使水提液澄明,且后两者还能够在降低浸膏得、保留有效成分、保证制剂稳定性方面优于前者,并能缩短生产周期,降低成本。

The observation of SEM, EDAX and IR analysis showed that the conformation of MPPE was changed, the surface and section of MPPE were rough and accidented, the specific surface area of MPPE was increased, and magnetic powders, HAP and other additives were separated in polyethylene polymer. The removal rates of Phenol by using PPE and MPPE were 82%, 93%, the removal rates of TOC could reach 78%, 89% respectively, when the phenol wastewater at 120mg/L concentration was treated with aerated process for 14 hours. The removal rates of Phenol were 85%, 90%, the removal rates of TOC reached 80%, 86% respectively when the phenol wastewater at 350mg/L was treated for 35 hours. The weight of biofilm formation on MPPE was 64% and 34% higher than that on PPE at this two wastewaters.

质量浓度为120mg/L的含苯酚废水在好氧生物填料柱中处理14 h后,PPE和MPPE对苯酚去除分别为82%和93%,TOC去除分别为78%和89%;质量浓度为350mg/L的含苯酚废水处理35h后苯酚去除分别为85%和90%,TOC去除分别为80%和86%;两种不同质量浓度的含苯酚废水降解后MPPE上微生物挂膜量分别比PPE提高约64%和34%。

With different carrier filling factors, the volume load will have different influence on the system. The research results indicated that in the reactor with 7.5%carrier filling factor, whenthe amount of material supplied is 3.2L/d,the capacity aerogenesis rate will be the maximum1.15L/L·d and the COD removing rate rises by 47.38%.It shows the good anti-impact loadperformance.

不同载体填充下进行的容积负荷对系统的影响研究结果表明:7.5%载体填充反应器在进料量为3.2L/d时可获得最大容积产气1.15L/L·d,并在此负荷下的COD去除也高达47.38%,表现出了良好的抗冲击负荷的性能。

An experimental investigation into the effects of pure Ag and AgAu alloys sheath materials on the properties of Bi (2223) multifilamentary tapes is presented in this paper. The experimental results show that the drastic reduction of thermal conductivity and the increase of resistivity by the addition of Au into the Ag matrix can be achieved.

Bi(2223)/Ag及Bi(2223)/AgAu高温超导多芯带材低温下的热导和横向电阻实验研究结果表明,采用AgAu合金代替纯银作为包敷材料可以显著降低Bi(2223)高温超导多芯带材的热导和提高横向电阻

Results The mutation incidence of LKG was 22.96%, while the mean incidence of GPG was 7.59%(The incidences of C20-OH group, glucoside group and aglucon group were 5.32%, 6.25% and 11.00% respectively). When treated with DMBA for 4 to 8 weeks, the mutation incidence of LKG ranged from 11.10% to 40.00%, while GPG ranged from 0 to 10.70%.

结果 白斑癌变模型组突变达22.96%,绞股蓝处理组平均为7.59%;绞股蓝各组抗突变效佳依次为C20-OH组、总甙组、甙元组,其突变分别为5.32%、6.25%、11.00%;DMBA诱导癌变4~8周时白斑模型组突变为11.10%~40.00%,绞股蓝处理组为0~10.70%。

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