猪的一胎

Cast, the number of hawks or falcons cast off at one time, usually a pair; cete, a company of badgers; covert, a flock of coots; covey, a family of grouse, partridges, or other game birds; drift, a drove or herd, especially of hogs; exaltation, a flight of larks; fall, a family of woodcock in flight; gam, a school of whales, or a social congregation of whalers, especially at sea; kennel, a number of hounds or dogs housed in one place or under the same ownership; kindle, a brood or litter, especially of kittens; litter, the total number of offspring produced at a single birth by a multiparous mammal; muster, a flock of peacocks; nide, a brood of pheasants; pod, a small herd of seals or whales; pride, a company of lions; rout, a company of people or animals in movement, especially knights or wolves; school, a congregation of fish, or aquatic mammals such as dolphins or porpoises; shrewdness, a company of apes; skein, a flight of wildfowl, especially geese; skulk, a congregation of vermin, especially foxes, or of thieves; sloth, a company of bears; sord, a flight of mallards; sounder, a herd of wild boar; stable, a number of horses housed in one place or under the same ownership; swarm, a colony of insects, such as ants, bees, or wasps, especially when migrating to a new nest or hive; troop, a number of animals, birds, or people, especially when on the move; warren, the inhabitants, such as rabbits, of a warren; watch, a flock of nightingales; and wisp, a flock of birds, especially of snipe.

cast 指一次所放飞的鹰或隼的数目，通常为一对： cete，一群； covert 一群大鹋； covey，一群松鸡、石鸡或其它作猎物的鸟； drift，一群或一堆，尤指家猪； exaltation，一群云雀； fall，一群飞行中的丘鹬； gam，一群鲸或一伙捕鲸人，尤指在海上的； kennel，一群住在一起或属同一个人所有的猎狗或狗； kindle，一窝或一巢，尤指小猫： litter，一产多胎的哺乳动物一次所生的幼仔的总数； muster，一群孔雀； nide，一窝野鸡； pod，一小群海豹或鲸； pride，一群狮子； rout，一群在行进中的人或动物，尤指骑士或狼； school，一群鱼或水生哺乳动物如海豚或小鲸； shrewdness，一群猿； skein，一群猎鸟，尤指鹅； skulk，一群害兽或坏人，尤指狐狸或小偷； sloth，一群熊； sord，一群野鸭； sounder，一群野公猪； stable，住在同一处或属同一个人所有的一群马； swarm，一群昆虫，如蚂蚁、蜜蜂或黄蜂，尤指当它们迁往一个新的巢或蜂房时； troop，一群动物、鸟或人，尤指在行进中时； warren 养兔场的一群动物，如兔子； watch 一群夜莺； wisp 一群鸟，尤指鹬鸟参见同义词 crowd

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分为4个部分，第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究，确立了一种较好的培养方法：与颗粒细胞共培养，找到了一种适合猪卵母细胞体外成熟的培养基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸，成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%，国外报道的最高成熟率为(85.7±4.1)%；第二部分对猪卵母细胞孤雌激活的化学方法进行了研究，确立了化学激活猪卵母细胞的两种最佳方法：1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min，再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中，卵裂率和桑囊胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min，再与8mM的DTT共孵育30min，卵裂率和桑/囊胚形成率为(81.0±2.8)%和(39.6±2.7)%；第三部分对孤雌激活胚胎的培养条件进行了研究，确立了一种最佳的胚胎培养条件：在SOF简单培养基中添加颗粒细胞进行前3天的培养，然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养，其桑椹胚和囊胚的发育率为(59.5±3.2)%；第四部分研究了IGF-I

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失