猪的

Through Liaoning Province Taian bills farmers first 35 suspected cases of classical swine fever diagnostic, recorded incidence of death from the process and will have a record of 11 symptoms, and so Autopsy examination step analysis, and later in the Shenyang Agricultural University Veterinary College pastoral guidance from the laboratory diagnosis, through production observation, classical swine fever virus onto the world body immune reaction, Enzyme linked immunosorbent assay testing, and made with Erysipelas ,Swine plague, Streptococcosis suis , and Mycoplasmal pneumomia of swine, Swine Toxoplasmosis , and ultimately determine the reasons for the cause of classical swine fever virus incidence.

本人通过对辽宁省台安县某农户的35头猪瘟疑似病例进行诊断，记录了从发病到死亡的过程，并且将所出现的症状一一记录，对死亡的猪挑选了3头进行剖检做近一步分析，对疑似病症进行分析排除。之后提取病料并保存，在沈阳农业大学畜牧兽医学院赵玉军教授的指导下进行了实验室诊断，通过涂片观察、猪瘟病毒兔体交互免疫反应、酶联免疫吸附实验，并做出了与猪丹毒、猪肺疫、猪气喘病、猪链球菌、弓形虫病的鉴别诊断，最终确定为此次引起发病的原因为猪瘟病毒。

To evaluate the effect of PRRSV on humoral and cellular immune responses,threeweek old SPF piglets and conventional piglets with antibody to PRRSV were inoculated nasally with PRRSV BJ 4 strain,and immunized intramuscularly with hog cholera vaccine 48 hours postinoculation.

对 2 0日龄 SPF猪和 2 0日龄猪繁殖与呼吸综合征血清阳性猪，人工感染猪繁殖与呼吸综合征病毒北京分离株( BJ-4 )后 4 8h接种猪瘟疫苗，利用 ELISA方法检测仔猪针对 PRRSV和猪瘟疫苗的体液免疫，利用 MTS法检测仔猪外周血单核细胞对有丝分裂原 Con A的刺激反应。

According to the gene frequency of M307 of FUT1 gene in different pig breeds, based on Neis genetic distance, two unrooted consensus trees were constructed using both Neighbor-joining method and UPGMA method.

由NJ系统发生树可见，所有的国内地方猪种首先聚为一个大的类群，而含有外来猪种血缘成分的猪种和所有的外来猪种与国内地方猪种遗传距离较远。

The furthest genetic distance appeared between Nuogu Pig and Guanling Pig, and the closest showed between Nuogu Pig and Kele Pig.

群体间遗传距离最远的是关岭猪与糯谷猪，最近的是柯乐猪与糯谷猪。

The experiment obtains clone pig HUMMLC2B gene (GenBank logs onto date: DQ533994), use PCR-RFLP technology, analysed HUMMLC2B gene the 1st embedded child medium Msp Ⅰ is enzymatic cut much condition (T613C) is in distributinging; analysed the much condition sex in 7 breed pig 5 many condition sex and 36 long white pigs, groups 104 pigs and grow to be mixed in vain 5, the result makes clear, the scale that waits for a gene and B to wait for a gene frequency except the A in long white pig in detected swinery is 1 ∶ 2 outside, of the others all take absolutely advantage for a gene such as B, and a gene such as A is pure close individual detect in long white pig only.

实验获得克隆猪HUMMLC2B基因(GenBank登录号：DQ533994)，并采用PCR-RFLP技术，分析了HUMMLC2B基因第1内含子中的MspⅠ酶切多态(T613C)在7个品种猪中的多态性分布；分析了多态性和36头长白猪、5个群体104头猪以及长白和5个群体之和的140头猪胴体性状和肉质性状间的相关，结果表明，在检测的猪群中除长白猪中A等位基因和B等位基因频率的比例为1∶2外，其余的均为B等位基因占绝对优势，且A等位基因纯合个体只在长白猪中检测到。

Fresh food choices Guinea pigs will not eat what they don't like: some cavies have very discriminating tastes, while others will eat anything that you put in front of them.

新鲜的食物中猪猪不吃什么，它们不喜欢的：一些猪猪很挑食，而另一些猪猪会吃你摆在它们前面的任何东西。

The blood relationship tree and evolution position of Tibet mini-pig were established. Result There were 14 mutation sites between domestic pigs in China and pigs from Europe. Besides there was a significant difference in two nucleotide site: a T→C switch in site 420 and the G→A switch in site 883 at the same time.

结果 西藏小型猪等国内部分品种猪的Cyt b基因序列与欧洲猪相比有14个变异位点；但是西藏小型猪与国内品种猪相比存在两个特殊碱基位点即在420位点T→C转换的同时在883位点存在G→A转换。

The results show that: 1 supplementation of protein to maturation media improves cumulus expansion in vitro compared to the protein-free control, but cumulus expansion is not necessarily related to oocyte nuclear maturation in pigs, and cumulus expansion is not the criterae for determination of nuclear maturation of pig oocytes, but only the exclusion of the first polar body; 2 exposure of pig COCs to hormone supplements for 23-24 hours improved cumulus expansion but had no significant effect on nuclear maturation compared to that for 46-48 hours; 3 under our research conditions, supplementation of different proteins into different maturation media has different effects on porcine oocyte nuclear maturation, but has no significant effect on subsequent embryonic development after IVF; 4 the nuclear maturation rates of pig oocytes matured in mTCM+pFF and mNCSU+pFF are superior than that in mNCSU+FCS; 5 different maturation media have no effect on pig oocyte cumulus expansion and subsequent embryonic development after IVF.

结果显示：(1)在成熟液中添加蛋白质可以加强卵丘细胞的扩散，但猪卵母细胞的核成熟与其周围的卵丘细胞扩展没有必然的联系，卵丘细胞扩散或成放射状不宜作为猪卵母细胞核成熟的标准，只有排出第一极体才能作为猪卵母细胞核成熟的标志；(2)在猪COCs的46-48小时成熟培养的后23-24小时阶段去除成熟液中的激素不但可以保证卵母细胞的核成熟率，而且可加强卵丘细胞的扩散；(3)在现有实验条件下，在mTCM和mNCSU中添加10％pFF与在mNCSU中添加10％FCS相比可获得较高的猪卵母细胞核成熟率；(4)在不同的成熟液中添加不同的蛋白质对猪卵母细胞核成熟率的影响效果不一样，但对体外受精后的早期胚胎发育影响不明显；(5)成熟液种类对猪卵母细胞的卵丘细胞扩散和体外受精后的早期胚胎发育无显著影响。

The homology of the Myostatin sequences was analyzed among species. The results showed that the mature region DNA for Myostatin of Jinhua pig has the similarity of 100%% 97%, 95%, 95%, 93%, 93%, 93%, 89%, 90%, 89%, 87%, 87%, 86%, 86%, 86%, 85% and 76% compared with that of pig, horse, human, monkey, cow, hare, goat, sheep, house mouse, rat, goose, turkey, chicken, pigeon, Xiaoshan chicken, Fan duck and zebrafish respectively. The mature region DNA for Myostatin of Xiaoshan chicken has the identity of 99%, 97%, 93%, 92%, 92%, 87%, 86%, 86%, 86%, 86%, 85%, 85%, 85%, 84%, 84%, 83% and 74% compared with that of chicken, turkey, pigeon, goose. Fan duck, monkey, human, horse, pig, Jinhua pig, house mouse, rat, sheep, hare, goat, cow and zebrafish respectively. The mature region DNA for Myostatin of Fan duck has the homology of 91%, 92%, 92%, 90%, 95%, 84%, 84%, 85%, 85%, 85%, 83%, 81%, 81%, 83%, 81%, 82% and 73% compared with that of chicken, Xiaoshan chicken, turkey, pigeon, goose, monkey, human, horse, pig, Jinhua pig, house mouse, rat, sheep, hare, goat, cow and zebrafish respectively.

将测序结果与GenBank上其它物种的相应序列进行同源性分析，结果显示，金华猪Myostatin与哺乳动物猪、马、人、猴、牛、兔、山羊、绵羊、小家鼠和大鼠的同源性分别为100％、97％、95％、95％、93％、93％、93％、89％、90％和89％，与鸟类鹅、火鸡、鸡、鸽子、萧山鸡和番鸭的同源性分别为87％、87％、86％、86％、86％和85％，与鱼的同源性为76％；萧山鸡Myostatin与鸟类鸡、火鸡、鸽子、鹅和番鸭的同源性分别为99％、97％、93％、92％和92％，与哺乳动物猴、人、马、猪、金华猪、小家鼠、大鼠、绵羊、兔、山羊和牛的同源性分别为87％、86％、86％、86％、86％、85％、85％、85％、84％、84％和83％，与鱼的同源性为74％；番鸭Myostatin与鸟类鸡、火鸡、鸽子、鹅和萧山鸡的同源性分别为91％、92％、90％、95％和92％，与哺乳动物猴、人、马、猪、金华猪、小家鼠、大鼠、绵羊、兔、山羊和牛的同源性分别为84％、84％、85％、85％、85％、83％、81％、81％、83％、81％和82％，与鱼的同源性为73％。

During 1992-1995, the listeriosis is found in cheese and meat in France, and from 2001, our import quality check institute regularly check import food produce, many times they checked out listeriosis and salmonella in the meat produce from more than 20 international food production company of American, Canada, France, English, Belgium and Denmark, such as in pig kidney, pig stomach, pig ear, and chop/short rib.

1992-1995年法国出产的奶酪及猪肉中发现李斯特菌，2001年11月以来，我国质检部门多次从美国、加拿大、法国、爱尔兰、比利时、丹麦等二十多家肉类加工厂进口的猪腰、猪肚、猪耳、小排等三十多批近千吨猪副产品中检出单增李斯特菌、沙门氏菌等致病菌。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。