猪瘟

The above result revealed that an optimum surrounding temperature was necessary when the rabbits were to be used for CSFV production. In addition, the subject rabbits should avoid heliosis during the late production process and transportation.

上述结果表明在猪瘟兔化弱毒脾淋疫苗生产中，GMP生产动物房必须给兔子提供最舒适的室温，原料兔在生产后期和运输过程中也应避免中暑。

But atypical HC and inapparent CSF began to appear after 1980s, which had resulted in great loss.

但自上世纪80年代以来，我国开始出现了温和、非典型性猪瘟。

It was very impossible that those mutations of GX epidemic strains of CSFV can cause the incompleted protection of HCLV vaccine.

GXLZ与GXNN和HCLV、Shimen的氨基酸序列对比发现，在E_2基因B、C高变的抗原区，GXLZ、GXNN在710、713、725、孙石静：广西猪瘟流行毒株E。

With the method of reverse transcription-polymerase chain reaction and nest PCR, P80 gene of CSFV is cloned from the cells infected by the strain Shimen of csfv, and connected with eukaryotic expression vector pEGFP-C1. Then recombined eukaryotic expression plasmid pEGFP-P80 of P80 gene is achieved successfully. It builds the basis for expressing and gaining protein p80 in mammiferous cells.

利用反转录PCR和套式PCR技术，从猪瘟病毒Shimen株细胞毒中克隆出P80 基因，并进一步把P80 基因连接在真核表达载体pEGFP-C1 上，成功地构建出P80 基因重组真核表达质粒(pEGFP-P80)，为在哺乳动物细胞中表达并纯化出p80 蛋白奠定了基础。

Great losses to pig industry worldwide have been caused by the prevalence of CSF, especially of nontypical forms.

猪瘟的流行给世界养猪业的发展和贸易造成了巨大损失。

The study researched the genetic diversity of E2 gene of classical swine fever virus that poly-passed in the swine umbilical vein endothelial cells to reveal the virus's pathopoiesis mechanism.

研究猪瘟病毒E2基因在猪脐静脉血管内皮细胞中多次传代后的遗传变异情况，为CSFV的致病机理研究提供理论依据。

Standard laboratory methods are based on virus isolation, serologic detection and Polymerase chain reaction.

目前猪瘟病毒的检测方法主要有病毒分离法、血清学检测、PCR等检测方法。

The result shows: 3bring content to be able to detect from inside vaccinal sample to the pollution that supports former put oneself in another's position to pcr, 3 pairs of check that make thin

结果显示：3对pcr引物都可以从疫苗样品中检测到支原体的污染，3对引物的检出率分别为20%、17.1%和14.3%，说明猪瘟活疫苗中的支原体污染为多种支原体的混合污染，污染源较为复杂。

The RT-nPCR can be used to differentiate pigs infected with wild-type CSFV from those vaccinated with the C-strain vaccine. It thus minimizes the possibility of miss-killed vaccinates during outbreaks.

本研究建立的RT-nPCR可以有效区分猪瘟强毒和弱毒，减少了未感染的免疫猪被误杀的可能性。

Structural and envelope glycoprotein E2 (gp55) of Classical swine fever virus is the most antigenic protein being responsible for eliciting neutralizing antibodies and conferring protective immunity. Infection of cells with CSFV is mediated by the interaction of E2 and Erns with the cell surface receptor.

猪瘟病毒囊膜表面结构糖蛋白E2 (gp55)是诱导机体产生中和抗体及激发保护性免疫应答的主要抗原蛋白。E2和Erns与细胞表面受体的相互作用介导病毒对细胞的感染过程。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失