猪瘟

The prospects for research and application of the diagnostic assays were discussed,which have implications for the detection and prevention of ASF.

在预防控制非洲猪瘟方面，对上述检测技术和诊断方法的应用进行了讨论和展望，旨在为研究该病提供理论参考。

Nowadays Mexican is said to be cursed with a prevailing swine fever.

据说眼下墨西哥正遭殃受苦发猪瘟。

Routine detecting techniquefor cell immunity such as white blood count and differential, lymphocyte blastogenesis test has beenused, and combine with flow cell technology to detect the level of cell immunization after advancedimmunization at several important aspect, and tracking experiments to observe changes in the numberof the WBC and lymphocytes, lymphocyte proliferation, the dynamic changes of CD4~+ and CD8~+lymphocyte subsets andγ-IFN; and detected dynamic changes rules of IgG, IgM in humor andmucosa with indirect ELISA, then compare with ordinary immunization methods.

主要采用白细胞计数和分类计数、淋巴细胞转化试验等常规细胞免疫检测技术结合先进的流式细胞技术从细胞免疫的几个重要方面入手检测猪瘟超前免疫后细胞免疫的水平，并进行跟踪试验，观察其白细胞、淋巴细胞数目变化情况，淋巴细胞增殖情况，CD~(4+)和CD~(8+)淋巴细胞亚群及γ-IFN的动态变化规律；同时通过间接ELISA方法检测体液和黏膜中各IgG、IgM的动态变化规律，同时与普通免疫方法进行比较。

Within seven years, basically eliminate a number of those diseases most harmful to human beings and livestock, such as schistosomiasis, filariasis, bubonic plague, encephalitis, cattle plague and hog cholera

十二在七年内，基本上消灭若干种危害人民和牲畜最严重的疾病，例如血吸虫病、血丝虫病、鼠疫、脑炎、牛瘟、猪瘟等。

In this paper a summary of research advances in biologicalcharacteristics of hog cholera virus in recent years has been described.

本文综述了近年来对猪瘟病毒的生物学特性研究的一些新进展。

The pig is the only animal in which hog cholera is known to occur naturally.

猪是已知自然发生猪瘟的唯一动物。

Hog cholera,as good as dead.

猪瘟干的，就像死神一样。

Through DOT-blot hybridiztion the result indicated SA215 and SA215 were of the same distribution on animal ,simply planting the following trigeminal ganglion .21-day-old SPF pigs inoculated intramuscularly SA215 strain could be protected from being affected at day 28 after oronasal challenge-exposure with 106 PF.U high-dose virulent PRV Fa strain and at day 42 after i.m.

SAZ巧株lml接种21日龄健康仔猪(伪狂犬病和猪瘟检测阴性)，在接种后28天用10毕FU的PRV Fa强毒株进行滴鼻攻毒，42天用猪瘟强毒SM株血毒lml肌肉注射攻击，结果表明接种猪能抵御两次病毒攻击，而同条件下对照结果成立，表明SAZ 15株具有良好免疫原性。

The Erns gene,which is the first envelope protein,was amplified from the spleen of rabbits which were infected with classical swine fever virus by RT-nPCR method.

从兔脾组织中提取猪瘟病毒兔化弱毒株RNA，利用RT-PCR技术，扩增了猪瘟兔化弱毒的第一个囊膜糖蛋白基因Erns，并对其进行了序列分析。

The recombinant fowl poxvirus rFPV-E0-E2 was serially passaged for 20 passages in specific-pathogen-free chicken embryo fibroblast culture. The passage 5, 10, 15 and 20 were chosen to identify by blue plaque assay, PCR amplification, gene sequence analysis and indirect immunofluoresence assay.

将重组鸡痘病毒rFPV-E0-E2在鸡胚成纤维细胞上连续传20代，取第5、10、15和20代重组病毒进行以下检测：用蓝斑试验鉴定各代重组病毒纯度；用PCR方法扩增猪瘟病毒E0、E2基因，进行基因序列分析；用间接免疫荧光实验检测各代重组病毒中猪瘟病毒E0、E2基因的表达。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失