猪瘟

The Classical Swine Fever is caused by CSFV, a kind of virus epidemic disease with acute , hot and high contiguous infectious , which can be prevented and cured by butchery, insulate and the immunity inoculates. Vaccine of C-stub has following disadvantages: it must conserve in refrigerate and the atavism of the vaccine was often observed on immune pigs, which will affect the prevention of the CEF.

猪瘟是由猪瘟病毒引起的一种急性、热性和高度接触传染的病毒性传染病，其防治主要采取屠杀、隔离和免疫接种。C-株疫苗的主要缺点是需要冷冻保存以及免疫动物容易恢复致病，这将影响猪瘟的防治。

Through Liaoning Province Taian bills farmers first 35 suspected cases of classical swine fever diagnostic, recorded incidence of death from the process and will have a record of 11 symptoms, and so Autopsy examination step analysis, and later in the Shenyang Agricultural University Veterinary College pastoral guidance from the laboratory diagnosis, through production observation, classical swine fever virus onto the world body immune reaction, Enzyme linked immunosorbent assay testing, and made with Erysipelas ,Swine plague, Streptococcosis suis , and Mycoplasmal pneumomia of swine, Swine Toxoplasmosis , and ultimately determine the reasons for the cause of classical swine fever virus incidence.

本人通过对辽宁省台安县某农户的35头猪瘟疑似病例进行诊断，记录了从发病到死亡的过程，并且将所出现的症状一一记录，对死亡的猪挑选了3头进行剖检做近一步分析，对疑似病症进行分析排除。之后提取病料并保存，在沈阳农业大学畜牧兽医学院赵玉军教授的指导下进行了实验室诊断，通过涂片观察、猪瘟病毒兔体交互免疫反应、酶联免疫吸附实验，并做出了与猪丹毒、猪肺疫、猪气喘病、猪链球菌、弓形虫病的鉴别诊断，最终确定为此次引起发病的原因为猪瘟病毒。

Hog cholera is infectious diseases of world-wide economic importance. Classical swine fever virus is the causative agent of hog cholera.

猪瘟是严重影响养猪业生产的烈性传染病之一，猪瘟病毒（classical swine fevervirus，CSFV）是引起猪瘟的致病原。

CSFspread severely in our country and about 39.65% of pigs died of disease died of CSF. The economic lossesreach a hundred million RMB. The Hog cholera lapinized vires vaccine was developed in 1956 in ourcountry and was accepted the most safe CSF vaccine using all over the world. Many countries haveeliminated the CSF by this vaccine.

我国是猪瘟流行较严重的国家，每年因猪瘟死亡的猪约占病死猪的39.65％，经济损失达100亿元左右。1956年我国成功的研制出猪瘟兔化弱毒株疫苗，此疫苗被认为是最安全的疫苗而被全世界广泛的采用，许多国家应用此疫苗消灭了猪瘟。

Multitudes of domestic and abroad researchers made a lot of study on the humoral effect of CSFvaccine and hold the same result that the pigs immunized by Hog cholera lapinized virus vaccine canproduce neutralizing antibodies and provide a complete protection for CSF.

众多国内外学者对猪瘟疫苗的体液免疫效果做了大量的研究，均认为我国的猪瘟兔化弱毒苗能够刺激机体产生中和抗体，对猪瘟病毒攻击完全保护。

Each group was vaccinated with Hog cholera tissue vaccine, cell vaccine or spleen vaccine with three dose levels (1×, 2×or 3×recommended doses).

随机分成9组，每组又分为3个小组，每小组3头，分别免疫猪瘟组织苗、猪瘟细胞苗和猪瘟脾淋苗，各疫苗分别采用1头份、2头份和3头份3个剂量。

At present, Chinas main method control classical swine fever is vaccine injection, that artificialactive immunization, As for it is no final conclusion that the difference of domestic CSF immuneeffects with advanced immunization and ordinary routine immunization. And it will take enormousmanpower and resources for farms to take advanced immunization, and there is a possibility of certainanaphylactic response. It is not easy extensive, the immune programme of each hoggery is difference.

目前我国防治猪瘟主要采取注射猪瘟疫苗的人工主动免疫方法，由于国内对猪瘟超前免疫和普通常规免疫两种方法在免疫效果上的差别没有定论，且养殖场采取超前免疫措施需要大量的人力及物力，且存在一定的过敏反应，不容易广泛开展，因此各个养猪场所采取的猪瘟免疫程序各不相同。

The results are as follows:In all the detected copies,the number which swine fever antibody level of serum is less than or equal to the ratio of 1 to 8 is 267,and the immunity percent of pass is 75.5%.

哺乳仔猪检测 10 7份，猪瘟血清抗体水平≤ 1∶ 8的有 34份，免疫合格率为 6 8%。断奶仔猪检测 333份，猪瘟血清抗体水平≤ 1∶ 8的有 12 3份，免疫合格率为 6 2 %。育肥猪检测 34 0份，猪瘟血清抗体水平≤ 1∶ 8的有 94份，免疫合格率为 72 %。

In summary, under the circumstances that protect of swine plague vaccine for sow, the differenceof immune response effect between advanced immunity and normal immunity is not quiet. This willprovide evidence and reference not only for illuminate the reaction mechanism of humoral immunity,mucosa immunity and cell immunity reduced by swine plague vaccine, but also for laboratory reseach,immunoprophylaxis and integrate control.

这将为进一步阐明猪瘟疫苗免疫所诱导的体液、黏膜和细胞免疫反应机制，进而为猪瘟的试验室研究及目前猪瘟的免疫预防及综合防治提供一定的理论依据和参考。

This review covered the following aspects in the development and application of CSF DNA vaccines: construction and evaluation, application of adjuvants, combination with other vaccines and the existing problems and solutions.

以下从猪瘟DNA疫苗的构建和评价、佐剂在猪瘟DNA疫苗中的应用、猪瘟DNA疫苗与其他疫苗的联合应用以及目前猪瘟DNA疫苗存在的问题和解决途径等方面做了比较全面的阐述。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！