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猪流行性感冒

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Was presented in 12 cases (24%) by bacterial isolation. By in-situ hybridization, PCV2 signals were chiefly distributed in interstitial and necrotic lesions, and the positive signals could be found in macrophages-like cells and necrotic debris. PRRSV signals were mainly located at interstitial lesions and alveolar wall, and the positive signals could be found in macrophages-like cells and epithelial cells of alveoli. Swine influenza virus could be found in interstitial and necrotizing lesions, and the signals could be found in macrophages-like cells, and epithelial cells of terminal bronchiole and alveoli.

另运用原位杂交法对PCV2、PRRSV及猪流行性感冒病毒进行检测,结果发现PCV2主要分布於PNP的间质及坏死病灶区,并能於肺泡及终末细支气管上皮细胞、巨噬细胞与坏死细胞碎片皆可见有病毒核酸;PRRSV则多分布於间质病灶区及肺泡壁处,能於巨噬细胞与肺泡上皮细胞见到病毒核酸;SIV则存在於间质及坏死病灶区,且能於肺泡及终末细支气管上皮细胞与巨噬细胞见有病毒核酸。

With the pig breed and breath ask for virus integratedly (the cause of disease that prrsv) happening mixes or afterwards hair contracts is microbial, include a pig streptococcic, deputy pig is bloodsucking bacillus, pig is pneumonic a former put oneself in another's position, haemorrhage defeats courage and uprightness pleural and pausteur bacterium, pneumonic bloodsucking bacili and pig grippe virus.

和猪繁殖和呼吸综合征病毒发生混合或继发感染的病原微生物,包括猪链球菌、副猪嗜血杆菌、猪肺炎支原体、出血败血性巴斯德氏菌、胸膜肺炎嗜血杆菌和猪流行性感冒病毒。

In situ hybridization and immunohistochemisty for the formalin-fixed, paraffin-embedded lung tissues and RT-PCR for homogenates of lung tissues were applied for this purpose.

故在本研究中,尝试著去开发对於猪流行性感冒病毒更快速的检测及分型方法,同时也希望探讨病毒分布与肺脏病灶之间的相关性。

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