猪丹毒

Erysipelothrix rhusiopathiae is a gram-positive bacterium that causes erysipelas in animal and erysipeloid in humans.

猪丹毒杆菌是一种革兰氏阳性菌，会造成动物的丹毒症与人的类丹毒。

With simultaneous salmon ella or swine erysipelas infection, the spleen may be enlarged and dark

由于同时感染沙门氏菌病或猪丹毒，脾脏可能增大和发黑。

Vaccination in place: Pig temperature, swine erysipelas, lung disease are unified in the period cut-Yan immunization.

防疫到位：猪温、猪丹毒、猪肺疫均在阎割期统一防疫。

Prevention of Disease in place: Pig temperature, swine erysipelas, lung disease are cut at Yan Unity quarantine period.

防疫到位：猪温、猪丹毒、猪肺疫均在阎割期统一防疫。

Prevention of Disease in place: Pig temperature, swine erysipelas, lung disease are unified in the period cut-Yan immunization.

防疫到位：猪温、猪丹毒、猪肺疫均在阎割期统一防疫。

Vaccination in place: Pig temperature, swine erysipelas, lung disease are cut at Yan Unity quarantine period to eliminate the sale of sick pigs.

防疫到位：猪温、猪丹毒、猪肺疫均在阎割期统一防疫，杜绝病猪出售。

Through Liaoning Province Taian bills farmers first 35 suspected cases of classical swine fever diagnostic, recorded incidence of death from the process and will have a record of 11 symptoms, and so Autopsy examination step analysis, and later in the Shenyang Agricultural University Veterinary College pastoral guidance from the laboratory diagnosis, through production observation, classical swine fever virus onto the world body immune reaction, Enzyme linked immunosorbent assay testing, and made with Erysipelas ,Swine plague, Streptococcosis suis , and Mycoplasmal pneumomia of swine, Swine Toxoplasmosis , and ultimately determine the reasons for the cause of classical swine fever virus incidence.

本人通过对辽宁省台安县某农户的35头猪瘟疑似病例进行诊断，记录了从发病到死亡的过程，并且将所出现的症状一一记录，对死亡的猪挑选了3头进行剖检做近一步分析，对疑似病症进行分析排除。之后提取病料并保存，在沈阳农业大学畜牧兽医学院赵玉军教授的指导下进行了实验室诊断，通过涂片观察、猪瘟病毒兔体交互免疫反应、酶联免疫吸附实验，并做出了与猪丹毒、猪肺疫、猪气喘病、猪链球菌、弓形虫病的鉴别诊断，最终确定为此次引起发病的原因为猪瘟病毒。

But Streptococcus suis type 2 often combined infectionwith some other etiological agents as Actinobacillus suis, Bacillus erysipelatous andPRRS virus, It results in tremendous difficulty by clinical diagnosis and antibodiesdetection.

但猪链球菌经常与其他一些病原如猪放线杆菌、猪丹毒杆菌和猪蓝耳病病毒等发生混合感染，给临床诊断和抗体水平的检测造成了很大困难。

Methods The SpaA was purified by electroelution from NaOH-extracted antigen of Erysipelothrix rhusiopathiae strain C43311. The rSpaA-N was expressed in E. coli BL21 as a soluble protein by IPTG inducing,and purified with GST affinity chromatography. Mice of each group were subcutaneously immunized three times with 50μg or 100 μg of native SpaA, rSpaA-N or NaOH-extracted antigen with in complete or incomplete Freund adjuvant at 2-week intervals.

将猪丹毒丝菌C43311株SpaA-N以可溶形式表达在大肠杆菌BL21中，用GST Bind Resin纯化试剂盒纯化rSpaA-N，采用电洗脱法从猪丹毒丝菌C43311株NaOH提取抗原中纯化天然SpaA，将rSpaA-N、天然SpaA和NaOH提取抗原制成亚单位疫苗，同时设GST及生理盐水对照组，间隔2周分3次皮下免疫小鼠，第3次免疫后2周用100LD50猪丹毒丝菌C43065株进行腹腔攻毒，采用间接ELISA方法检测免疫组小鼠血清的抗体动态变化。

Longtengísales company one vaccination in place: Pig temperature, swine erysipelas, lung disease are cut in Yan Unity quarantine period to eliminate the sale of sick pigs.

防疫到位：猪温、猪丹毒、猪肺疫均在阎割期统一防疫，杜绝病猪出售。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！