猪

Firstly, we performed pulpotomy for posterior teeth and pulpectomy for anterior teeth. And the experiments were divided into four groups. In the first group, nothing was put in the pulp chamber or canals. In the second group, deciduous dental pulp stem cells of mini-pig with cultured medium were transplanted into the pulp chamber and pulp canals. In the third group, deciduous dental pulp stem cells of mini-pig with lovastatin were transplanted. In the fourth group, deciduous dental pulp stem cells of mini-pig with odontoblast induction medium were transplanted.

在动物实验中，我们将迷你猪乳牙的前牙作拔髓，后牙做断髓处理，各分成四组做实验，第一组不放任何细胞、第二组加入迷你猪乳牙牙髓干细胞和培养液、第三组加入迷你猪乳牙牙髓干细胞和lovastatin，第四组加入迷你猪乳牙牙髓干细胞和odontoblast induction medium，观察三个月后，以探讨其牙齿组织是否会再生。

Below brief introducer the pig is streptococcic 2 model infection and lab diagnose research progress. 1 epidemiology pig is streptococcic is a kind of pathogen that can affect pig and person, normally the nasal cavity of pig of consist in health is mixed inside tonsil, also inside body of animal of consist in other.

下面简要介绍人猪链球菌2型感染及实验室诊断探究进展。1流行病学猪链球菌是一种能够感染猪和人的病原体，通常存在于健康猪的鼻腔和扁桃体内，也存在于其它动物体内。

Incorporat ed with the sequences of other Suidae species obtained from GenBank, the phyloge ntic trees of Suidae species were constructed by Neighbor-Joining and Most Pars imony method.

结合从GenBank中获得的其他猪科物种胞色素b序列，用邻接法和最大简约法构建了猪科物种的系统发育关系，其结果显示苏拉威西鹿猪与其他猪科物种为姐妹群的关系。

Examine both your guinea pig and any cavies it was housed with; here is what you should look for

检查你选中的猪猪还有和它同住一笼的猪猪，下面是你应该寻找的

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分为4个部分，第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究，确立了一种较好的培养方法：与颗粒细胞共培养，找到了一种适合猪卵母细胞体外成熟的培养基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸，成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%，国外报道的最高成熟率为(85.7±4.1)%；第二部分对猪卵母细胞孤雌激活的化学方法进行了研究，确立了化学激活猪卵母细胞的两种最佳方法：1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min，再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中，卵裂率和桑囊胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min，再与8mM的DTT共孵育30min，卵裂率和桑/囊胚形成率为(81.0±2.8)%和(39.6±2.7)%；第三部分对孤雌激活胚胎的培养条件进行了研究，确立了一种最佳的胚胎培养条件：在SOF简单培养基中添加颗粒细胞进行前3天的培养，然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养，其桑椹胚和囊胚的发育率为(59.5±3.2)%；第四部分研究了IGF-I

I liked my piggy girl's cooking indeed for her perfect meals and dishes. But more, I actually enjoyed the time with her, looking at her liked a little busy housewife, listening to her chatter that I was eating just liked a pig ate Ginseng Fruit and the chaos of my room as Pigsties……, all of these make me so happy.

我很喜欢阿猪的手艺，因为她菜烧的好，饭做的好，但是我更为之不能自拔的是和阿猪在一起的感觉，看着她象个小家庭主妇一样的忙里忙外，听她数落我吃饭是猪八戒吃人参果，说我的房间象猪窝……内心是无比的幸福。

Staff of the "pig child" dirty pigsties to the beautiful sea, towering mountains, noisy street eye-opener, long to see her step by step with the new social environment to foster the survival of her life Ability, but because of the "pig child," Wang Feng significant contact with pigs for a long time, every time a new thing in the face when she knows nothing about that always surprised, excited, happy, or to issue a pig Hengheng sound.

工作人员把&猪孩&从肮脏的猪圈带到美丽的大海、巍峨的高山、喧闹的街道开眼界、长见识，让她一步步熟悉新的社会环境，培养她衣食住行的生存能力，但是由于&猪孩&王显凤与猪接触时间太长，每当她面对新鲜事物时总是茫然无知地表示惊讶、兴奋，或是惬意地发出猪的哼哼声。

The situation for pigs is no better. About half the world's pigs are crowded into airtight pigsties. Sows are incessantly forced into artificial pregnancy and they can only remain on their sides breastfeeding. Not yet grown, the piglets are forcibly separated from their mothers by butchers to be slaughtered and sold as suckling pigs.

猪的情况也好不到那里，世界一半左右的猪都挤迫在密不透风的猪圈里，母猪被迫不停的人工怀孕，产下小猪也只可以侧在笼内哺乳，小猪未成长又被强行与母猪分离，被屠夫宰成乳猪只售卖。

They conta i ned full genes ORFs and proved to have been prolongated effectively. The result showed that the seqences of 14 genes were genes known previously and 6 genes were new .We separately chose one seqence from known and the other from unknown genes to make full-length cDNA annotation. Their code number was EST contig110 and EST contig133. We designed specific primers to amplify the two genes by RT-PCR, and then sequenced. The result was obtained as expected and proved that silico cloning genes was reliable.

对20条contigs进行了基因注释，证实它们均得到了有效延伸，具有完整开放阅读框，其中已知的猪基因序列14条，猪新基因序列6条，分别从已知猪基因序列和未知猪基因序列中各抽取一条感兴趣序列进行全长cDNA的预测，它们的编号分别为EST contig110和EST contig133，设计特异引物对两条候选序列进行克隆与测序，结果与电子克隆所得序列一致，证实了电子克隆的有效性。

I, Shannon Blood Sausage. Stand-pig small intestine washed first with warm water to soak rice for half an hour, picked up the rice, adding blood, mix with the rice and put into a large stone or blood beater, then add green onion, salt, has been Net diesel refining pig, star anise powder mix, wash the pig has been poured into the small intestine, ligated at both ends into the pot, cook the back, pay attention to the fire can not be too fast and can not boil water, to keep turning in order to avoid open rupture Blood Sausage, and come to naught, cooked to salvage, you can cut consumption, increase can also be cooked pan fried dishes, the use of soy sauce, vinegar, monosodium glutamate pair of points is expected, it is delicious, Miao can not be made.

一、香血肠。将猪小肠洗净备用，先用温水将大米浸泡半个小时，捞起白米，加入猪血，与米搅匀，放入大石磨或打浆机磨成血米浆，再加入葱花、盐、已炼的猪网油渣、八角粉等拌匀，灌入已洗净的猪小肠中，结扎两端，放入锅内，慢火煮熟，注意火不能太猛，水不能烧开，要经常翻动，以免血肠破裂开，而前功尽弃，煮熟后捞起，即可切块食用，也可用菜锅加熟油煎后，用酱油、醋、味精配成的点料，更是味香可口，妙不可言。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！