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To characterize the isotype of human xenoreactive nat ural antibodies against porcine pancrease islet cells, cyostat sections of the p ancrease were incubated with the sera from 18 cases of IDDM or 20 human controls respectively, further with FITC-conjugated goat antihuman IgG or IgM secondary antibodies. IgG was found to bind to pancrease islet cells in sera from 8 cases of IDDM and 8 human controls, while IgM in 4 IDDM and 6 human controls.

为检测人抗胰岛细胞天然抗体的同种型,采取18份胰岛素依赖型糖尿病患者血清及20份正常人血清分别与杂种胰腺组织的冰冻切片孵化后,再用免疫荧光标记的羊抗人IgG、IgM单克隆抗体染色。8份IDDM血清和8份正常人血清中含有直接针对胰岛细胞的IgG型抗体;4份IDDM血清和6份正常人血清中含有IgM 型抗胰岛细胞抗体。

Porcine was acted as the experimental animal. It was researched on problem of the origin of obtaining early embryonic cells, methods of separating blastomere cells, best parameter and order of ECNT embryo electric activation and system of ECNT embryo IVC, which was to find a scientific method of fitting ECNT and a procedure of fitting the lab producing embryos largely, optimize the procedure of ECNT and improve the ECNT embryo in vitro.

本实验以为实验动物,对胚胎细胞核移植供体的来源、卵裂球细胞分离方法、核移植重建胚的电激活最佳参数、核移植受体的激活时序以及核移植重建胚体外培养系统相关问题进行了研究,旨在探索一种适用于胚胎细胞核移植的科学方法,提高胚胎细胞核移植胚胎的体外发育率,优化胚胎细胞核移植程序。

Stability and an established routine will keep stress levels down, and make the cavy more comfortable.

稳定的,确定的日常模式会降低的紧张程度,让觉得舒适。

Analysis showed that the magnitude and direction of the heterosis effects were different among combinations. Moreover, there were higher heterosis which were beneficial on improving carcass and meat quality in the 1/4 Laiwu than that in the other crossbreed.

分析表明,含不同比例莱芜血缘的杂交组合间存在大小和方向不同的杂种优势效应,并且,含1/4莱芜血缘的杂交比其他杂交存在着较大的有利于胴体和肉质性状改善的杂种优势效应。

Chinese medicine is also useful to eat, you can eat at meat, the best drink spots like soup;; eat at pig soup, carp soup can, ah, I is not much,,, c months, twice the time to eat like a pig soup, wash your pig boiling add water directly, and not to put too much salt, preferably both on the net forepaw pig

吃中药也管用,,,你可以多吃点肉类,,最好多喝点汤之类的;;多吃点脚汤,,鲫鱼汤也可以啊,,我也是不多,,,三个月的时候吃了两次脚汤就好了,,你把脚洗净加水直接煮,,不要放太多的盐,,最好是净上的俩前蹄

The ORF2 gene is the most important protein-code gene for porcine circovirus, and is the main gene differing porcine circovirus typel from porcine circovirus type2 (PCV2). To construct a specific PCR detecting method, we designed a pair of primers to amplify a partial fragment of ORF2 gene from PCV2, referring to fifteen strains complete genome published in Genbank. The PCR product was about 0.67kb in size detected by gelose gel electrophoresis.

圆环病毒的开放阅读框2(ORF2)是其主要结构蛋白的编码基因,也是区分圆环病毒1型(PCV1)和圆环病毒2型(PCV2)的重要基因,本研究参照GenBank已发表的15株PCV2基因序列,设计合成一对引物,对PCV2的ORF2基因进行PCR扩增,产物经琼脂糖凝胶电泳,呈现一条大小约0.67kb的特异条带,经条件筛选,建立了病料中PCV2感染的PCR检测方法。

To evaluate the effect of PRRSV on humoral and cellular immune responses,threeweek old SPF piglets and conventional piglets with antibody to PRRSV were inoculated nasally with PRRSV BJ 4 strain,and immunized intramuscularly with hog cholera vaccine 48 hours postinoculation.

对 2 0日龄 SPF和 2 0日龄繁殖与呼吸综合征血清阳性,人工感染繁殖与呼吸综合征病毒北京分离株( BJ-4 )后 4 8h接种瘟疫苗,利用 ELISA方法检测仔针对 PRRSV和瘟疫苗的体液免疫,利用 MTS法检测仔外周血单核细胞对有丝分裂原 Con A的刺激反应。

Most of the published suid fossils are reviewed and suggested a new systematic position now. The relationship of Chinese fossil suoids with that from Indian subcontinent and Europe are carefully discussed, the new understanding of Paleogeography and paleoecology during the Neogene of China are proposed.

通过对各时期我国发现的形类化石的重新研究,厘定了我国所有重要形类化石的分类,并对中国形类与印巴大陆、欧洲大陆形类的关系进行了详细的探讨,对古地理、古气候提出了新的见解。

Our project is the first one in China that focus on the suoids, which discuss about all the known suoid fossils found from Eocene to Pliocene in China. The origination, evolution, paleoecology and paleogeography are the key topics in the project, and thus the project is a compositive study of Chinese fossil suoids.

本项目是国内首个以形类为内容的专题研究,涉及了我国境内从晚始新世到上新世发现的几乎所有重要的形类化石,涉及到形类的起源、进化、生态、古地理等方面的研究,是对中国形类较为全面系统的综合性研究。

The pig's wife, his neighbors, and his children forgot him. It was as if such a pig had never even lived in the world.

的老婆,的邻居,的儿女也忘记了他,仿佛从来没有这么一头的世界里活过。

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