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Additionally,this study developed three species-spcific real-time fluorescence quantitative PCR-based assays for rapidly, specificity, sensitivity,detecting Brucella abortus, Brucella melitensis, Brucella suis.

在建立布鲁氏菌属荧光定量PCR检测方法的基础上,根据布鲁氏菌基因组多拷贝插入元件IS711及其下游多态位点,设计了检测牛、羊、种布鲁氏菌的引物和牛、羊、种特异性荧光探针,优化了牛、羊、种布鲁氏菌荧光定量PCR反应体系和条件,建立了牛、羊、种布鲁氏菌荧光定量PCR检测方法,组装了牛、羊、种布鲁氏菌检测试剂盒,该方法能特异检测上述3个种的布鲁氏菌。

One night the big bad wolf, who dearly loved to eat fat little piggies, came along and saw the first little pig in his house of straw. He said "Let me in, Let me in, little pig or I'll huff and I'll puff and I'll blow your house in!""Not by the hair of my chinny chin chin", said the little pig. But of course the wolf did blow the house in and ate the first little pig.

一天晚上,一只早就想吃胖小的大灰狼,来到第一只小用稻草建的房子前,他说,"让我进去,让我进去小,不然我就吹掉你的房子""不要废话"小说但是狼真的吹破了房子,吃掉了第一只小

The deep fried chicken cheese set is also very good, the surface is crispy n the chicken meat is soft n juicy too but not much chicken taste maybe its because the cheese has covered the taste of it.

这点在没比较下很难说明,简单的说,扒没""味,可能是扒本身质素差,又或是厨师为了让扒松化而作了令""味流失的加工。

RAPDs were used to study the genetic divergency and phylogenetic relationship of Chinese wild boar and 5 breeds or strains of domestic pigs, including Erhualian pig, Hongdenglong pig, Jiangquhai pig, Xiang pig and Yorkshire.

对二花脸、红灯笼、姜曲海、香、约克夏及华东地区野共24个个体进行了随机扩增多态DNA分析,以探讨家与野间的亲缘关系。

Through Liaoning Province Taian bills farmers first 35 suspected cases of classical swine fever diagnostic, recorded incidence of death from the process and will have a record of 11 symptoms, and so Autopsy examination step analysis, and later in the Shenyang Agricultural University Veterinary College pastoral guidance from the laboratory diagnosis, through production observation, classical swine fever virus onto the world body immune reaction, Enzyme linked immunosorbent assay testing, and made with Erysipelas ,Swine plague, Streptococcosis suis , and Mycoplasmal pneumomia of swine, Swine Toxoplasmosis , and ultimately determine the reasons for the cause of classical swine fever virus incidence.

本人通过对辽宁省台安县某农户的35头瘟疑似病例进行诊断,记录了从发病到死亡的过程,并且将所出现的症状一一记录,对死亡的挑选了3头进行剖检做近一步分析,对疑似病症进行分析排除。之后提取病料并保存,在沈阳农业大学畜牧兽医学院赵玉军教授的指导下进行了实验室诊断,通过涂片观察、瘟病毒兔体交互免疫反应、酶联免疫吸附实验,并做出了与丹毒、肺疫、气喘病、链球菌、弓形虫病的鉴别诊断,最终确定为此次引起发病的原因为瘟病毒。

The pig is a curiosity in Muslim Afghanistan, where pork and pig products are illegal because they are considered irreligious, and has been in quarantine since Sunday after visitors expressed alarm it could spread the new flu strain.

在阿富汗穆斯林是珍品,在哪儿类产品是违法的,因为是被认为没有宗教信仰的,自星期天游客表示了可能会传播新的流感链之后,这头就被隔离检疫了。

When you are called as follows: pigs, piglets, pig, silly pig, bungy, Zhu Baobao ...

当有人称你为:、小头、傻、笨宝宝…的时候。

If you suspect that one of your cavies is ill, it is wise to isolate the cavy immediately as a precaution against infecting the rest of your herd.

如果你怀疑中的一只生病了,明智的做法是立即隔离这只,以防止病症传染给其他的

The results show that: 1 supplementation of protein to maturation media improves cumulus expansion in vitro compared to the protein-free control, but cumulus expansion is not necessarily related to oocyte nuclear maturation in pigs, and cumulus expansion is not the criterae for determination of nuclear maturation of pig oocytes, but only the exclusion of the first polar body; 2 exposure of pig COCs to hormone supplements for 23-24 hours improved cumulus expansion but had no significant effect on nuclear maturation compared to that for 46-48 hours; 3 under our research conditions, supplementation of different proteins into different maturation media has different effects on porcine oocyte nuclear maturation, but has no significant effect on subsequent embryonic development after IVF; 4 the nuclear maturation rates of pig oocytes matured in mTCM+pFF and mNCSU+pFF are superior than that in mNCSU+FCS; 5 different maturation media have no effect on pig oocyte cumulus expansion and subsequent embryonic development after IVF.

结果显示:(1)在成熟液中添加蛋白质可以加强卵丘细胞的扩散,但卵母细胞的核成熟与其周围的卵丘细胞扩展没有必然的联系,卵丘细胞扩散或成放射状不宜作为卵母细胞核成熟的标准,只有排出第一极体才能作为卵母细胞核成熟的标志;(2)在COCs的46-48小时成熟培养的后23-24小时阶段去除成熟液中的激素不但可以保证卵母细胞的核成熟率,而且可加强卵丘细胞的扩散;(3)在现有实验条件下,在mTCM和mNCSU中添加10%pFF与在mNCSU中添加10%FCS相比可获得较高的卵母细胞核成熟率;(4)在不同的成熟液中添加不同的蛋白质对卵母细胞核成熟率的影响效果不一样,但对体外受精后的早期胚胎发育影响不明显;(5)成熟液种类对卵母细胞的卵丘细胞扩散和体外受精后的早期胚胎发育无显著影响。

The homology of the Myostatin sequences was analyzed among species. The results showed that the mature region DNA for Myostatin of Jinhua pig has the similarity of 100%% 97%, 95%, 95%, 93%, 93%, 93%, 89%, 90%, 89%, 87%, 87%, 86%, 86%, 86%, 85% and 76% compared with that of pig, horse, human, monkey, cow, hare, goat, sheep, house mouse, rat, goose, turkey, chicken, pigeon, Xiaoshan chicken, Fan duck and zebrafish respectively. The mature region DNA for Myostatin of Xiaoshan chicken has the identity of 99%, 97%, 93%, 92%, 92%, 87%, 86%, 86%, 86%, 86%, 85%, 85%, 85%, 84%, 84%, 83% and 74% compared with that of chicken, turkey, pigeon, goose. Fan duck, monkey, human, horse, pig, Jinhua pig, house mouse, rat, sheep, hare, goat, cow and zebrafish respectively. The mature region DNA for Myostatin of Fan duck has the homology of 91%, 92%, 92%, 90%, 95%, 84%, 84%, 85%, 85%, 85%, 83%, 81%, 81%, 83%, 81%, 82% and 73% compared with that of chicken, Xiaoshan chicken, turkey, pigeon, goose, monkey, human, horse, pig, Jinhua pig, house mouse, rat, sheep, hare, goat, cow and zebrafish respectively.

将测序结果与GenBank上其它物种的相应序列进行同源性分析,结果显示,金华Myostatin与哺乳动物、马、人、猴、牛、兔、山羊、绵羊、小家鼠和大鼠的同源性分别为100%、97%、95%、95%、93%、93%、93%、89%、90%和89%,与鸟类鹅、火鸡、鸡、鸽子、萧山鸡和番鸭的同源性分别为87%、87%、86%、86%、86%和85%,与鱼的同源性为76%;萧山鸡Myostatin与鸟类鸡、火鸡、鸽子、鹅和番鸭的同源性分别为99%、97%、93%、92%和92%,与哺乳动物猴、人、马、、金华、小家鼠、大鼠、绵羊、兔、山羊和牛的同源性分别为87%、86%、86%、86%、86%、85%、85%、85%、84%、84%和83%,与鱼的同源性为74%;番鸭Myostatin与鸟类鸡、火鸡、鸽子、鹅和萧山鸡的同源性分别为91%、92%、90%、95%和92%,与哺乳动物猴、人、马、、金华、小家鼠、大鼠、绵羊、兔、山羊和牛的同源性分别为84%、84%、85%、85%、85%、83%、81%、81%、83%、81%和82%,与鱼的同源性为73%。

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Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了

The enrich the peopling of Deng Xiaoping of century great person thought, it is the main component in system of theory of Deng Xiaoping economy, it is a when our country economy builds basic task important facet.

世纪伟人邓小平的富民思想,是邓小平经济理论体系中的重要组成部分,是我国经济建设根本任务的一个重要方面。