猪

This experiment first studied in the mature nutrient medium to increase the hormone to the porine ovocyte in vitro mature process in the chromosome configuration and the mature rate influence, by explored the porine ovocyte in vitro mature rule, enhanced in vitro raise maturity, was the ROSI technology system preparation high quality ovocyte; Then has carried on the experiment in view of the porine round sperm cell in vitro separation, through to the ROSI operation and ICSI (Intracytoplasmic sperm injection, the comparison which ICSI) operated studies has affected the ROSI operation the factor.

本试验首先研究了成熟培养液中添加激素对猪卵母细胞体外成熟过程中染色体构型和成熟率的影响，以探索猪卵母细胞体外成熟规律，提高体外培养成熟度，为ROSI技术体系准备优质卵母细胞；然后针对猪圆形精细胞的体外分离进行了试验，进而通过对ROSI操作和ICSI(Intracytoplasmic sperm injection，ICSI)操作的比较研究了影响ROSI操作的因素。

Pigs can be infected by avian influenza and human influenza viruses as well as swine influenza viruses. When influenza viruses from different species infect pigs, the viruses can reassort i.e.

禽流感，人流感以及猪流感病毒都会对猪造成感染；当不同种来源的流感病毒同时感染时，病毒之间就会重新&洗牌&，从而产生一种新的&禽人猪&混合病毒。

Pigs can be infected by avian influenza and human influenza viruses as well as swine influenza viruses. When influenza viruses from different species infect pigs, the viruses can reassort (i.e. swap genes) andnew viruses that are a mix of swine, human and/or avian influenza viruses can emerge.

禽流感，人流感以及猪流感病毒都会对猪造成感染；当不同种来源的流感病毒同时感染时，病毒之间就会重新&洗牌&，从而产生一种新的&禽人猪&混合病毒。

Pigs can be infected by avian influenza and human influenza viruses as well as swine influenza viruses. When influenza viruses from different species infect pigs, the viruses can reassort (i.e. swap genes) and new viruses that are a mix of swine, human and/or avian influenza viruses can emerge.

禽流感，人流感以及猪流感病毒都会对猪造成感染；当不同种来源的流感病毒同时感染时，病毒之间就会重新&洗牌&，从而产生一种新的&禽人猪&混合病毒。

Pigs can be infected by avian influenza and human influenza viruses as well as swine influenza viruses. When influenza viruses from different species infect pigs, the viruses can reassort (i.e. swap genes) and new viruses that are a mix of swine, human and/or avian influenza viruses can emerge.

禽流感，人流-以及猪流感病毒都会对猪造成感染；-当不同种来源的流感病毒同时感染时-病毒之间就会重新&洗牌&，从而产生一种新的&禽人猪&混合-毒。

Pigscan be infected by avian influenza and human influenza viruses as wellas swine influenza viruses. When influenza viruses from differentspecies infect pigs, the viruses can reassort (i.e. swap genes) and newviruses that are a mix of swine, human and/or avian influenza virusescan emerge.

禽流感，人流感以及猪流感病毒都会对猪造成感染；当不同种来源的流感病毒同时感染时，病毒之间就会重新&洗牌&，从而产生一种新的&禽人猪&混合病毒。

Maxi-break - the entire herd from weaning to slaughter is depopulated, and repopulation carried out from weaning up.

大中断–将整个猪群清空，包括从断奶到屠宰阶段的猪只。然后重新再从断奶仔猪开始转入新猪。

Rapid amplification of cDNA end:Retroverse transcription product of total RNA extracted from normal porcine tissue was used as the template,gene specific primers were designed and advantage 2 polymerase mix was used in PCR,of which using porcine genomic DNA as the template:forward primer was designed according to the acquired consensus region of human and pig FGL2 3′ sequences while reverse primer was designed from human FGL2 3′ end downstream sequence;TA cloning.

以猪正常小肠及心脏组织提取新鲜总RNA，反转录后作为模板，设计基因特异性引物，采用Advantage 2 聚合酶混合物进行PCR扩增；依据猪与人FGL2基因3′端已知同源序列设计PCR上游引物，以人FGL2基因3′末端序列设计下游引物，以猪基因组DNA为模板采用Advantage 2 聚合酶混合物进行PCR反应；PCR载体重组质粒DNA亚克隆扩增。

Therefore, we selected the 5 genes, as well as SMTN gene related to human cardiovascular diseases, for chromosomal mapping, CDS cloning and analysis, spatio-temporal distribution, mutation riddling, and association analysis with production traits of 3 porcine populations. We expect to know the structure and function of these 6 genes primarily, and supply data for porcine marker assistant selection of improving meat production.

因此，本研究选择NADH呼吸链相关基因，以及与人类心血管疾病相关的SMTN基因，以猪的骨骼肌为研究对象，用人×仓鼠体细胞杂种克隆板进行6个基因的染色体定位、CDS克隆和序列分析、用半定量RT-PCR进行时空表达谱分析、进行突变位点的筛选、并利用3个猪群的产肉性状资料进行基因型与性状的关联分析，以期初步了解这些基因的结构和功能，并为改进猪产肉性能的标记辅助选择提供资料。

He pulls down trousers, preparing to insert when going in, the dog runs back to the buttock that will bite him again, he has gone out dog kick rustily far, but, the pig gives again run, such, he catchs a pig over and over, the pig runs over and over, finally, this person lies tiredly on the ground, was asleep.

他拉下裤子，正准备插进去时，狗又跑回来咬了他的屁股，他恼火地把狗踹出去好远，但是，猪又给跑掉了，就这样，他一遍又一遍地抓猪，猪一遍又一遍地跑掉，最后，这人累的躺在地上，睡着了。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失