狂犬病的

The main regions (405-1 146 nt) in the glyco pro tein gene of 8202,BRV and MRV,isolated from deer,cattle and mouse in China re spectively,of rabies virus was amplified by reverse transcriptase-polymerase ch ain reaction,cloned and sequenced.

对来源于鹿、牛和鼠的狂犬病病毒野毒株82 02、BRV、MRV的G基因405～1 146位核苷酸序列进行了RT-PCR扩增、克隆和序列测定，并应用计算机软件对这3个毒株的测定序列和人源株CGX89的相应序列进行了分析比较。

As you mentioned, with most reported long incubation periods (especially from countries with enzootic canine rabies) there is always a question about other more recent exposures than the !0causative!1 exposure reported. Generally speaking, most incubation periods fall within 20-90 days following exposure, with the incubation period decreasing with the severity of the bite and proximity to the head.

你提到的，大多数的长潜伏报道（特别是犬类狂犬病盛行的国家），总是有一个问题的，就是真正引起患者死亡的，是新近一次感染，而不是所报道的那次感染。

The RT-PCR was sensitive and specific for HEV and did not amplify closely related bovine coronavirus and pseudorabies virus of pigs.

检测与HEV亲缘性较高的牛冠状病毒及猪的伪狂犬病毒均为阴性，最低可以检测到10个TCID50/100μl的病毒，说明该方法的有较好的特异性和敏感性。

His experiments supported the germ theory of disease also reducing mortality from puerperal fever and he created the first vaccine for rabies. He was best known to the general public for inventing a method to stop milk and wine from causing sickness - this process came to be called pasteurization.

他提出了以微生物代谢活动为基础的发酵本质新理论，发明了沿用至今的巴氏消毒法，成功地拯救了法国处于困境中的酿酒业、养蚕业和畜牧业；他发现传染病的微菌在特别的培养之下可以减轻毒力，从而发明了鸡霍乱疫苗、狂犬病疫苗，建立起了细菌理论。

It is designedthat a pair of primers are used in 3"-RACE, two pairs of nesting primers and one 5"phosphated primer are used in 5-RACE, eight pairs of general primers are used inPCR which cover all the genome of these two isolated rabies viruses.

根据已测定的狂犬病毒株全基因组序列，用分子生物学软件设计了用于3'-RACE的接头引物一对，用于5'-RACE的2对嵌套引物、1条5'磷酸化引物，用于普通PCR的8对引物，这些引物扩增序列覆盖了整个病毒全基因组序列，具有一定的重叠区域。

With the rabid albeit limp wristed group of squatters in the halls of power in Washington, war seems the only foreign policy they care to engage in. So called "free market capitalism" is impossible without a level playing field.

随著狂犬病尽管蹒跚wristed组的棚户区在大厅的权力在华盛顿，战争似乎是唯一的外交政策，他们从事护理个所谓的&自由市场资本主义&是不可能的了公平的竞争环境。

Results: Of the 709 interviewee, 574(80.96%) recognized rabies as a fatal disease, 156(22.00%) disregarded the threat posed by pet bites, 446 (62.91%) would seek medical assistance once bit, while 121(17.07%) reported treatment of wounds using improper, thumbnail approaches. Prevention was observed based on doctor's advices in all of 9 degree Ⅰ exposers (100.00%), 76 of 91 degree Ⅱ exposers (83.52%) and 32 of 212 degree Ⅲ exposers (15.09%).

结果：有80.96%(574/709)的被调查者知晓狂犬病是一种致死性疾病，22.00%(156/709)的被调查者对家养动物致伤表示无所谓、不用治疗的观点，认为只要致伤需就诊者占62.91%(446/709)，用土方法等不正确方法处理伤口者占17.07%(121/709)；100.00%(9/9)的Ⅰ级暴露者，83.52%(76/91)的Ⅱ级暴露者和15.09%(32/212)的Ⅲ级暴露者完全按照医嘱进行了预防治疗。

The nucleotides sequence and deduced amino acid sequence of these cDNA figments mentioned above were divided into two fractions. One is carbon part of L gene , the other is 5 terminal,and they were compared with other rabies-related virus strains published in NCBI previously.The result show that the homology of nucleotide sequence and amino acid sequence of carbon part in L gene among these two rabies virus strains is 91.3% and 95.8%, and these two strains belong to type of gene I.

本实验以吉林鹿源DRV8202株及河南鼠源野毒株MRV的L基因的C端为主要研究内容，用RT-PCR和3'-RACE方法对其进行了克隆和测序，将所测得的核苷酸序列和推导的氨基酸序列分成两个部分，一是L基因C端序列，另一个是L基因5'端的非编码区，并与NCBI上公开发表的狂犬病毒其它毒株的相应片段进行分析比较。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

Because virus may be absent or present at very low level in both clinical fluids, samples taken for ante-mortem diagnosis cannot definitively rule out rabies.

因为病毒可能在两者的临床液体的非常低的水平是缺席或者出现，为赌注-mortem 的诊断被拿的样品不能够决定性地排除狂犬病。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。