狂犬病

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

Rabies virus can be divided into seven serotypes now, and several rabiesviruses have not been classified.

目前狂犬病毒可分为7个基因型，还有几株新分离的病毒尚需确立分类地位。

There was no accumulative effect on immune protection in groups with mix plasmids.

这为猪伪狂犬病核酸免疫研制与应用提供了重要的实验依据。

If you have already been vaccinated before being bitten (the pre-exposure vaccine), two doses of the rabies vaccine will be given: one at the time of the bite, and one 3-7 days later.

如果您已接受注射前，被咬伤，两剂狂犬病疫苗，将给予：一是在时间的叮咬，和1 3-7天之后。

Anyone who handles possibly infected animals or is travelling to a risky area should have a pre-exposure anti-rabies vaccination, consisting of three injections over the course of a month.

任何人谁处理可能受感染的动物，或前往冒险的地区应该有一个接触前反狂犬病预防注射，其中3注资，超过的过程中一个月。

HK offers its volunteers who handle animals a rabies pre-exposure vaccination series.

协会为处动物的义工准备一系预防狂犬病疫苗注射。

The quarantine period is a precaution against the remote possibility that an animal may appear healthy, but actually be sick with rabies.

隔离期是为了预防一种微小的可能性，就是动物看似健康，但实际上已经感染狂犬病。

Is usually transmitted by a bite from a wild infected animal, such as a bat, raccoon, skunk, or fox.

狂犬病在美国一般是由野生被感染动物传递，比如蝙蝠，浣熊，臭鼬，或者狐狸。

Is usually transmitted by a bite from a wild infected animal, such as a bat, raccoon, skunk, or fox.

狂犬病在美国一般是由野生被感染动物传播，比如蝙蝠，浣熊，臭鼬，或者狐狸。

Methods Aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with pH 4, human immunoglobulin and human hepatitis B immunoglobulin for intravenous injection with pH 4/pasteurization,treatment on human fibrinogen and human coagulation factor Ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.

系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病毒，人血白作者：魏宪义，魏红，蹇远勇，陈海，刘文芳目的分析血液制品特定灭活因子的时效动力学曲线，科学性地评价与改进不合理的病毒灭活参数。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失