犰狳

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

Beyond that, there is an even bigger problem: the most suitable species to act as a host for a developing glyptodon embryo would be the far smaller 30-kilogram "giant" armadillo.

除此以外，科学家还面临一个更加棘手的问题：最适合担任雕齿兽胚胎的代孕妈妈的，可能是体重远远低于30公斤的&庞大的&犰狳。

Including Tung perch Section fish, white crayfish, the hole source, points viper, blind beetles, white eyes and flat worms, feet class , Isopoda, beasts in the bag mole Section, Mole Branch, Soricidae, Muridae capsule, small animals such as armadillos.

包括洞鲈科鱼、白色螯虾、洞源、穴蝰、盲甲虫、白色无眼扁形虫、双足类、等足类，兽类中的袋鼹科、鼹科、鼩鼱科、囊鼠科、小犰狳等动物。

There are over 20 species of armadillos.

有超过20种的犰狳。

There are over 20 species of armadillos.

犰狳有20多个种类。

Well, we've managed to avoid the armadillos and now we're at The Oasis.

好了，我们已经设法避开犰狳，到了绿洲餐馆了。

This is what makes coral reefs and armadillos out of bacteria, Darwin said -- accumulated microchange.

这种变化，达尔文说，就是让细菌变成珊瑚礁和犰狳的变化，也就是累进的微小变化。

Armadillos can move quickly even though it has short legs and are good swimmer; able to last 6 minutes underwater.

虽然犰狳的腿很短，它们跑得却相当快，而且它们还是游泳能手，能在水下坚持六分钟之久。

Yao doesn't like lassos and armadillos.

他不会喜欢牛仔们用的那些套索，也不会对犰狳（美国德州特有的一种动物）感兴趣。

There are over 20 species of armadillos. Here in the U.S., they're cute little things, about the size of a house cat.

目前美国犰狳大约有20个品种，他们的体形娇小，大小和家猫差不多大。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了