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Thyroid transcription factor-1 in normal, hyperplastic, and neoplastic follicular thyroid cells examined by immunohistochemistry and nonradioactive in situ hybridization.

本实验TTF-1在所有转移性腺癌中的敏感性为35.3%(18/51),特异性为100%(0/36),在肺腺癌中的敏感性为61.5%(16/26),特异性为91.7%(22/24),有2例肺外腺癌表达TTF-1,1例为乳腺癌,另1例为卵巢癌。

The method is based on real time reverse-transcription-PCR amplification of mRNA to detect viable Listeria monocytogenes by specific hlyA gene. This method can overcome the false-positive caused by amplification of nonviable Listeria monocytogenes in the detection process.

为克服传统PCR检测的假阳性问题,有效的检测单增李斯特活菌,本研究提取单增李斯特菌的总RNA并进行反转录,以单增李斯特菌的必要毒力基因hlyA为靶基因,设计特异性引物和TaqMan探针进行实时PCR检测,研究了检测的特异性和灵敏度,考查了对人工污染牛乳进行检测的应用性。

Results hPDLP wasisolated from human periodontium and most of the cells retained their fibroblastic spindle shape. hPDLP can be in-duced into osteoblast-like cells and adipocyte-like cells, and calcium deposition and lipid droplets were detected perspectively.

结果 第1代hPDLP的CD146和STRO-1阳性率分别是27.20%±3.98%和4.23%±4.08%;经矿化诱导可以形成矿化结节,有钙盐沉积;经成脂诱导可见特异性脂滴形成,特异性转录因子过氧化物酶体激活物增生受体2(PPARγ2)和脂蛋白脂酶表达上调。

The best accuracy was obtained for the diagnosis of AR with sensitivity and specificity of 80% and 79% for GB and 70% and 79% for PF, respectively.

诊断AR有最佳的准确性,对于GB的准确性和特异性分别为80%和79%,对于PF的准确性和特异性分别为70%和79%。

By way of comparison, detection of abscess, extraluminal air, and extraluminal appendicolith was 37.5% sensitive, 22.5% specific, and 32.5% accurate in diagnosing perforation, while identification of phlegmon was 40.0% sensitive, 95.2% specific, and 73.5% accurate in diagnosing appendiceal perforation.

通过对比,诊断阑尾穿孔时,脓肿、外漏气体、阑尾结石的诊断敏感性是37.5%,特异性是22.5%,准确性32.5%,然而诊断阑尾穿孔时阑尾蜂窝织炎的诊断敏感性是40%,特异性是95.2%,准确性73.5

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合,而且结合是饱和性的、可被竞争的,符合配体-受体结合的典型特征,同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性,表明水稻细胞质膜上存在nsLTP的受体;利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜,再进行结合实验,结合活性几乎不受影响;而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失,表明其受体为没有经过糖基化修饰的蛋白质;利用交联剂BS~3交联配体一受体后,再进行SDS-PAGE分离和放射自显影,结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合,可能是其受体;细胞外碱化实验表明,nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应,却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应,表明nsLTP和Cryptogein结合细胞膜上相同的位点,保护了植物细胞免受Cryptogein导致的细胞程序性死亡,并诱导系统获得性抗性的产生;体外Pull-down实验表明,nsLTP144和水稻的OsCaM1具有相互作用,暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

For sciatic blocks, cold sensation had a sensitivity of 77% and a specificity of 100%, whereas pinprick had a sensitivity of just 20% with a specificity of 100%.

而坐骨神经阻滞对温度刺激的 PFI 的敏感性和特异性为77%和100%、对痛觉刺激的 PFI 的敏感性和特异性分别为20%和100%。

This paper provided a statistical analysis of 2000 patients with allergicdiseases in Xi'an and the surrounding area.The results of the analysis show-edg:(1) the incidence of the allergic disease had little difference be-tween male and female, while it was higher in youth and children; respi-ratory allergy, skin allergy, and pollinosis were main types of the disea-ses;(2)the positive ratio of the skin tests of atopic inhalant allergenswas 66.4%,among which, mite, pollen, mold and house dust were high...

本文分析西安及其周围地区2000例变态反应病的情况,结果表明:两性发病率基本相同;青年期和儿童期为好发病期;过敏性呼吸道疾病、皮肤疾病及花粉症是主要发病类型;特异性吸入性过敏原皮试阳性率为66.4%,其中螨、花粉、霉菌的阳性率最高,室尘次之,是本地区过敏性疾病的主要过敏原;采用免疫治疗总有效率为90.15%,非特异性免疫调节治疗可增加免疫治疗效果。

The expressed proteins were purified on 15% SDS-PAGE, the specific protein gel was cut down, transferred onto nitrocellulose membrane and stained with ponceau for 10 minutes.

结果 获得1株含HIV-1 gp41基因的重组质粒,其蛋白的特异性表达为8%,经HIV-1阳性血清检测和国家标准参比血清检定,该表达蛋白灵敏性、特异性均为100%。

Compared with CT, MR imaging has sensitivity and specificity of 36% and 100%. Radiography has sensitivity and specificity of 70% and 96% respectively.

与CT相比,MRI敏感性和特异性分别为36%和100%,平片敏感性和特异性分别为70%和96%。

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