特异性

Design another set of specific primers, of which upstream and downstream primers were located in the two sides of the cleavage site respectively, to develop the one-step RT-PCR method for detecting H5 and H9 subtypes of AIV. Identify the sensitivity of the developed one-step RT-PCR method by detecting 2 AIV strains of H5 subtype and 3 AIV strains of H9 subtype, and the specificity by detecting the RNAs of NDV, IBV and IBDV as well as analogue detection using computer software.

根据H5、H9亚型HA基因序列，分别设计一套特异性引物，其上、下游引物分别位於裂解位点两侧，应用RT-PCR一步法检测AIV H5和H9亚型，并验证所建立方法的敏感性；通过检测NDV、IBV和IBDV等RNA病毒及电脑软件模拟检测，验证其特异性。

Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P.01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48 and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%. The overall sensitivity was significantly higher (P.05) than that of modified indirect MAIPA and that of using single antibody.

结果 特发性血小板减少性紫痕组4种羊抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异(P.01)；若将ITP组患者4种羊抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性，则流式微球技术检测血小板特异性自身抗体的敏感性为73.2%，特异性为94.3%；4种羊抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验(P.05)，且大于各单个抗体检测敏感性。

The parental BHK and the transfectants stably expressing human DR4, DR5, DcR1, or DcR2 were stained with control mouse IgG or murine antihuman DR4, DR5, DcR1, or DcR2 mAbs and analyzed by flow cytometry. Dotted and solid lines correspondent to control and specific stainings, respectiely. Cell surface staining with anti-DR4, anti-DR5 and anti-Fas mAbs.

抗人TRAIL受体的单克隆抗体的特异性：用小鼠IgG或鼠抗人DR4、DR5、DcR1及DcR2单克隆抗体对未转染的BHK细胞以及分别转染了人DR4、DR5、DcR1及DcR2并稳定表达的BHK细胞进行免疫组化染色，然后做流式细胞分析，点线和实线分别对应于对照及特异性染色。

Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P .01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48, and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%.

结果 特发性血小板减少性紫癜组4种单抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异（P＜0.01）；若将ITP组患者4种单抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性，则流式微球技术检测血小板特异性自身抗体的敏感性为73.17%，特异性为94.3%；4种单抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验（P＜0.05，且大于各单个抗体检测敏感性。

Final diagnoses at 1-year follow-up were RA in 31 patients, undifferentiated arthritis in 7 (5 self-limiting), and psoriatic arthropathy and antisynthetase syndrome in 1 patient each.

仅7例抗CCP抗体阳性，但最后均诊为类风湿关节炎，在该组血清阴性类风湿患者中的抗CCP阳性率仅23%(7/31)，其诊断特异性达100%(95%可信区间为71.7-100)，敏感性为23%(95%可信区间为9.6-41.1)，而用MRI标准则100%的类风湿患者诊断正确，另外有2例假阳性结果(1例银屑病关节炎和1例抗合成酶综合症)，MRI标准对诊断血清阴性类风湿关节炎的特异性为78%(95%可信区间为40.0-97.2)，敏感性为100%(95%可信区间为90.8-100)。

Results: In 40 patients with rectum or colon carcinoma proven by pathology, there were 6 cases with cecum carcinomas, 3 cases with ascending colon carcinomas, 3 cases with transverse colon carcinomas, 5 cases with descending colon carcinomas, 1 case with-sigmoid colon carcinomas and 22 cases with rectum carcinomas.

结果：病理证实结直肠癌40例（其中盲肠癌6例，升结肠癌3例，横结肠癌3例，降结肠癌5例，乙状结肠癌1例，直肠癌22例），多层螺旋CT检出了全部结直肠癌，敏感性为100%，总的分期准确率为80%(32/40)，B期分期准确率为83.3%(15/l8)，C期分期准确率为66.7%(8/12)，肿瘤浆膜外侵犯的敏感性和特异性分别为95%(36/34)和50%(2/4)，淋巴结转移的敏感性和特异性分别为60%(12/20)和90%(9/10)。

Rapid and reliable methods of virus universal detection are essential for fighting against bluetongue. We have therefore developed and evaluated a pair of primers which can detect various serotypes of BTV by RT-PCR.

对BTV进行快速、可靠的群特异性检测极为重要，因此本研究设计了适用于BTV群特异性检测的一对独特引物，并进行了评价。

Results:Monoclonal antibodies IH3,5A12,7E5,7F9 showed specific respo nse to NIH3T3-erbB2 cell and SKBR3 cell,no response to NIH3T3 cell.1H3 also boun d specifically to the extracellular domains of P185 protein that expressed by E.

结果：单克隆抗体1H3、5A12、7E5、7F9可与NIH3T3-erbB2细胞、SKBR3细胞特异性结合，与NIH3T3细胞无反应；1H3与原核表达的c-erbB2胞外区蛋白有特异性反应，而与无关蛋白KDR无反应。

Results showed that specific SIEA26~28 ku scFv with high level expression was achieved. The corresponding gene, ribosomal protein S4 was obtained by initially screening the cercaria cDNA library with the specific SIEA26~28 ku scFv. The obtaining of specific SIEA26~28 ku scFv lays the foundation for further screening and identification of the coding genes of SIEA26~28 ku, the natural molecular candidate vaccine against schistosomiasis.

结果显示，所获得的SIEA26~28ku特异性scFv，表达量高，采用该探针初步筛选出相关基因核糖体蛋白S4SIEA26~28ku特异性scFv的获得，为进一步筛选、分析鉴定抗日本血吸虫病天然分子候选疫苗SIEA26~28ku的编码基因奠定了基础。

Both specific and non-specific host defence mechanisms are responsible for the protection of fish against challenge infections with this ciliate.

鱼类的特异性和非特异性宿主防御机制都负责保护鱼类抗小瓜虫感染。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。