特异性

No immunostaining was seen in RPE. Double staining that Kir2. 1 and glutamine synthetase, Kir2. 1 and glutamine synthetase colocalized well. Intense Kir7. 1 immunolabeling was present on the apical surface of all RPE cells and appeared to extend over the length of the apical processes. Na〓, K〓-ATPase expression varied among RPE cells, but in highly expressing cells, it co-localized with Kir7. 1. Immunoreactivity of Kir2. 1、Kir4. 1 and Kir7. 1 acomplished by ABC immunohistochemistry in monkey and human retinae got the nearly same results as bovine.

间接免疫荧光组织化学显示，Kir2.1蛋白主要分布在Müller细胞与神经元相接触的细胞膜区域；与Müller细胞的特异性标记蛋白质抗谷氨酸合成酶抗体双重标记显示其分布特性重叠，在RPE未检测Kir2.1蛋白的免疫活性存在；Kir4.1蛋白集中分布于Müller细胞的内侧突起，与GS蛋白双重标记显示其分布特性重叠，RPE未检测Kir4.1蛋白的免疫活性存在；Kir7.1主要分布于RPE游离面及其突起的全长，与特异性标记RPE突起的Ezrin蛋白完全重叠，Na〓-K〓ATP酶在不同部位的RPE表达不同，Na〓-K〓ATP高表达的RPE细胞与Kir7.1的分布特性重叠。

After selecting a highly potent strain of poxvirus that was able to traffic to tumors when administered intravenously to mice the authors engineered the virus such that it would target only specific cancer cells -- those with increased expression of a protein known as E2F and/or activation of signaling downstream of a protein known as EGFR.

在选择高度特异性痘状病毒后才能有效的到达肿瘤生长地方，然后通过静脉途径给老鼠。这些病毒都是研究人员设计好的以至于只能到达特异性肿瘤细胞，像那些增加表达蛋白E2F和信号下游活化蛋白EGFR。

Methods The intra-dermal test and intranasal provocation test with ragweed pollen allergen, and specific serum IgE determination were given to patients with pollenosis. Results 85 patients were proved as ragweed pollensis in 502 patients with pollenosis based on the ARIA standard. They received step-treatment and specific immunotherapy.

在就诊的花粉症患者中，通过豚草花粉变应原皮内试验、鼻黏膜激发试验、血清特异性IgE测定等检测方法，按照&ARIA&关于变应性鼻炎新的分类标准进行了分类和阶梯式治疗及特异性免疫治疗。

The accordance rate of the two methods was 98.8%. Conclusion: To reduce the harm of syphilis to mothers and infant, we should generalize the combined method of the specific and nonspecific serology tests, and do TRUST titer test when positive.

为降低梅毒对妇婴的危害，应推广特异性血清学试验与非特异性血清学试验联合做为筛查，阳性的加做TRUST滴度。

The assay, which was based on primers and TaqMan-MGB probes selected from highly conserved regions of the hemagglutinin gene of avain influenza virus subtype H5, was optimized in a reaction system and a condition to improve the sensitivity, specificity and accuracy. In addition, colone technology was used to develop a quantitative PCR format with virus copies amount.

针对H5亚型禽流感病毒血凝素基因保守区域设计特异性引物与TaqMan-MGB荧光探针，筛选并优化荧光定量RT-PCR反应体系与反应条件，用以提高方法的特异性、敏感性与准确性；并通过体外克隆技术建立病毒基因拷贝数进行定量分析。

The results showed that (1) COX-2 mRNA and protein existed in the mature male rat testis. COX-2 was localized in spermatocytes by immunohistochemistry.(2) COX-2 also existed in adult man testis. COX-2 protein was positively stained in spermatocytes and Leydig cells.(3) 2 weeks after administration of rofecoxib, the level of testosterone in the whole testis reached its lower values, being only 50% of control values. However, testosterone level recovered during 4 weeks. After such treatment, histologic examination of these testes showed atrophy of the seminiferous tubules and maturation arrest of spermatogenesis.(4) 2 weeks post-EDS, expression of COX-2 decreased significantly (P.005), in comparison with vehicle-treatment control. 4 weeks after treatment, a new generation of fetal like Leydig cells repopulated in the testicular interstitium resulting in COX-2 expression partially recovered. Although the expression of COX-2 mRNA and protein are enhanced at 8th week after using external testosterone, it wasn't significant higher than control group.

实验研究证明：(1)正常成年雄性大鼠睾丸组织中COX-2在mRNA和蛋白质水平均存在表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞；正常成年男性睾丸组织中同样存在COX-2表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞和Leydig细胞；(2)服用特异性COX-2酶抑制剂rofecoxib 2周后，实验组大鼠睾丸组织内睾酮的含量减少，为正常对照组的50％；持续用药4周后睾丸组织内睾酮浓度逐渐恢复至正常水平；COX-2酶活性降低后病理组织切片显示睾丸内曲细精管萎缩，生精紊乱，持续用药4周时影响最明显；(3)注射特异性Leydig细胞杀灭剂EDS 2周后，实验组大鼠睾丸组织内睾酮浓度降至极低水平时，COX-2的蛋白和基因表达水平也显著低于正常空白对照组（P.005）；使用EDS后第4周，睾丸组织中的睾酮浓度逐渐回升，同样组织中COX-2蛋白表达和mRNA表达水平也相应提高接近正常水平；使用EDS后4周开始给予外源性睾酮，6周和8周时COX-2表达水平的绝对值虽有提高，但与正常大鼠空白对照组比较并无显著性差异，说明外源性雄激素刺激睾丸合成COX-2的作用并不明显。

Pedal swelling induced by 1% agar in mice, dermal c apillary hyper-permeability induced by histamine in rats were used to evaluate the anti-inflammatory of Kangning granules, and the clearance rate of intraveno us ch arcoal particles was used to study its effect on non-specific immunologic funct i ons. Serum haemolysin level and the number of antibody secretory cells in spleen were determined to assess its effects of specific immunologic functions.

运用小鼠足跖肿胀和大鼠皮肤毛细血管通透性实验观察其对炎症反应的影响；用碳粒廓清实验评价其对非特异性免疫功能的影响、测定免疫小鼠血清抗体水平和脾细胞抗体分泌细胞数量以评价感冒康宁颗粒对特异性体液免疫功能的影响。

Interaction between MAP kinase and MPF influerlces meiotic maturation and fertilization of oocyte throughout the animal kingdom, including stimulation of germinal vesicle breakdown, suppression of DNA replication, control of meiotic chromosome segregation, maintenance of metaphase Ⅱ arrest, and resumption and completion of second meiosis.

方法分别用MAPK激酶特异性抑制剂（U0126）和MPF特异性抑制剂抑制受精卵细胞MAPK和MPF的活性，通过同位素测定MAPK和MPF的活性变化，利用显微镜进行形态观察来判定MAPK活性变化对受精卵发育的影响。

Different B cells produce Ig molecules of different specificities and each B cell is capable of producing only one Ig specificity.

不同的 B 细胞所产生的免疫球蛋白具有不同的特异性，而第一个 B 细胞只能产生一种特异性的免疫球蛋白。

The specific inhibitors of PKA (H-89, 3 μmol/L) and PKG (KT-5823, 1 μmol/L) had no influence on the activation of TMP on BK channels.

PKA的特异性抑制剂H-89 (3μmol/L)和PKG的特异性抑制剂KT-5823(1μmol/L)对川芎嗪激活BK通道的作用无影响。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐