特异性

For evaluation of the hybridoma cells, immumofluorescence method with flow cytometer were used.

人骨髓间充质干细胞的特异性细胞表面抗原单克隆抗体可望在MCSs细胞的提纯和体外富集，特异性骨病的诊断等方面有广阔的应用前景。

Phosphatidylcholine-specific phospholipase C might play a key role in the signal transduction. The PC-PLC hydrolyses phosphatidylcholine to DAG. DAG is the second message in the cells and activates protein kinase C and mitogen-activated protein kinase.

磷脂酰胆碱特异性磷脂酶C在信号传递过程中具有重要作用，能特异性水解磷脂酰胆碱产生第二信使——二酯酰甘油，而DAG又可以激活蛋白激酶C，进一步把信号传递给丝裂原活化蛋白激酶，从而参与调控细胞增殖、分化和凋亡。

The present study investigated the effects of a specific COX-2 inhabitor(NS-398) and a selected PPARγagonist on the proliferation and apoptosis of pancreatic cancer SW1990 cell line to examine whether the two non-celltoxicity agents have synergistic anticancer role, so as to establish the experimental bases for application PPARγagonist associating with specific COX-2 inhabitor in treating pancreatic cancer.

本课题研究特异性COX-2抑制剂NS-398和PPARγ激动剂罗格列酮对人胰腺腺癌细胞系SW1990增殖、凋亡的影响，旨在明确两种非细胞毒性药物对胰腺癌有无协同抗癌作用，为临床联合应用特异性COX-2抑制剂和PPARγ激动剂防治胰腺癌提供实验依据。

To exam Kala - azar patients, sera and healthy persons, sera from epidemic area.

目的用从新疆不同地区分离得到的利什曼原虫前鞭毛体提取抗原，观察ELISA法检测黑热病病人和疫区健康人血清抗体时的敏感性和特异性，为制备ELISA诊断试剂盒和进一步提高这种检测方法的敏感性和特异性，提高黑热病现场流行病学调查结果的准确性和可靠性提供实验依据。

The results suggested that lymphokine might be spontaneously produced by normal mononuclear cells under culture condition in vitro.

一在体外培养条件下，淋巴细胞受特异性抗原或非特异性分裂原刺激后，可产生与释放淋巴因子。

It has been hypothesized that destruction of biliary tract in primary biliary cirrhosis would be mediated by autoreactive liver-infiltrating T cells through either cytotoxicity or lymphokine production.

抗原特异性T细胞与原发性胆汁性肝硬化(Primary biliary cirrhosis，PBC)中胆管特异性损伤密切相关，并在PBC的发生、发展中起重要作用。

The deleted mutant PAP gene was also cloned into yeast secreted expression pPIC9K vector to form pPIC9K~3, then the vector was transferred into Pachia pastoris GS115 strain. The specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50-60 U g per millilitre measured by UV-absorbed methods in the supernatant of the medium via high density fermentation. SDS-PAGE results showed that there was one protein band in the gel which molecular weight was about 34Ku . The protein could specific react with France PAP antiserum in Western-blotting. Activity tests revealed t

将缺失型PAP基因克隆于酵母分泌型表达载体PPICgK构成重组载体，然后导入毕赤酵母（P8chia nastoris）菌株GSlls细胞中，在甲醇的诱导下，经过酵母高密度发酵进行PAP的表达，经SDS－PAGE分析，结果表明，在培养基上清液中含有一明显的特异性蛋臼条带，大小为34Ku，经Western－blotting分析，该蛋白与法国PAP抗血清有特异性反应，体外活性检测表明该蛋白对TMV的侵染性具有高度的抑制性，说明该 PAP基因在毕赤酵母 GS中也得到了正确表达。

The uptake of 〓I-OxLDL in adipocytes was determined by gamma ray counter and normalized to protein concentration of each sample, was expressed as nanograms of 〓I-OxLDL uptake per milligram of cell protein. Reverse transcription polymerase chain reaction was used to evaluate PPARγ and CD36 mRNA expressions.

采用酶联免疫吸附法检测血浆IL-6以及脂肪细胞分泌IL-6的水平；采用gamma射线计数仪来检测脂肪细胞对〓I-OxLDL的特异性摄取情况，采用改良Lowry法进行细胞蛋白浓度测定，细胞对〓I-OxLDL的特异性摄取量以ng／mg细胞蛋白表示；采用逆转录聚合酶链式反应来检测PPAR γ和CD36 mRNA在脂肪细胞的表达情况。

It is to use a high degree of specificity of the antibody immune response and chromatography analysis technology, marked by colloidal gold β-HCG and mouse monoclonal antibody IgG and fixed in the nitro-cellulose membrane of the anti-α-HCG and sheep anti-mouse antibody IgG Antibody, the specificity of detection in urine HCG.

是利用高度特异性的抗原抗体反应及免疫层析分析技术，通过胶体金标记的β-HCG单克隆抗体和鼠IgG以及固定于硝基纤维素膜上的抗α-HCG抗体及羊抗鼠IgG抗体，特异性的检测尿液中HCG。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐