特异性

PCA is of specific significance for detecting the activity of rheumatic carditis and its specificity is based on the specific antigen from groups A streptococal membrane.

以链球菌菌膜为促凝刺激物的PCA试验在判断风湿心肌梗死性心脏炎方面具有特异性，其特异性取决于所用的刺激原为特异性抗原。

Results:The spective sensitivity value of cTnI,CK-MB and Mb were 93.3%,86.7% and 78.3%;the spective specificity value were 100%,100% and 88.0%;the sensitivity and specificity value of both cTnI and Mb were 96.7% and 88.0%;the sensitivity and specificity value of all cTnI,Mb and CK-MB were 98.3% and 88.0%;the positive and negative expectative value were 95.2% and 88.0%.

结果：患者入院后24 h内cTnI、CK-MB和Mb对AMI的敏感性分别为93.3%，86.7%和78.3%，特异性分别为100%，100%和88.0%；同时检测cTnI和Mb时AMI的敏感性为96.7%，特异性为88.0%；同时检测cTnI、Mb和CK-MB时AMI的敏感性为98.3%，特异性为88.0%，阳性预期值为95.2%，阴性预期值为88.0%。

Leishmania promastigotes antigens have good stability and heat-resistant nature. The sensitivity of 901 and 801 antigen were better, so we thought it could be used for serologic epidemiology investigation in XinJiang.

利什曼原虫前鞭毛体抗原有较好的稳定性，耐热性极好；901株、801株抗原的敏感性、特异性较好，可做为新疆血清流行病学调查所用的抗原株；801株的特异性更好，可用于黑热病的特异性抗体的检测及研究。

Part Ⅱ Objective: To study the immunoprotective and the immunotherapic effects of the new nucleic acid vaccine of the hepatitis B: pVAX1-C3d-S2S; respectively in normal and HBV transgenic mice after being inoculated intra- muscularly with this Eukaryotic Expression Plasmid, such as the in situ production of the expressed protein in muscle tissue section and specific humoral immune response in normal mice、the variation of specific antigen、antibody and HBV DNA, especialy the changes of specific antigen in liver.

第二部分新型乙肝核酸疫苗pVAX1 C3d-S〓S免疫预防效应和治疗作用的研究目的：研究含有新型分子佐剂补体C3d的HBV adw血清型的乙肝核酸疫苗重组真核表达质粒pVAX1-C3d-S〓S接种正常BALB／c小鼠和HBV转基因小鼠后，其免疫预防作用（接种局部肌肉中特异性抗原蛋白的表达和所诱生的体液免疫应答规律）以及免疫治疗作用（血清中特异性抗原的表达、特异性抗体的产生、HBV DNA的消长和肝组织中抗原表达的变化）。

In contrast, HCVpp-specific nAbs were readily detected in patients with chronic evolution and impaired virus-specific CD4 T-cell response but not in patients who cleared infection with robust virus-specific CD4 T-cell response.

相比之下，HCVpp特异性的中和抗体在HCV慢性感染和病毒特异性CD4 T细胞功能损伤的患者中较易测到，而在感染清除和病毒特异性CD4 T细胞反应强烈的患者中不易测到。

In contrast, HCpp-specific nAbs were readily detected in patients with chronic eolution and impaired irus-specific CD4 T-cell response but not in patients who cleared infection with robust irus-specific CD4 T-cell response.

相比之下，HCpp特异性的中和抗体在HC慢性感染和病毒特异性CD4 T细胞功能损伤的患者中较易测到，而在感染清除和病毒特异性CD4 T细胞反应强烈的患者中不易测到。

The results showed that Non-haemagglutinating activity was showed in the isolate. In agar diffusion test,there was a clear precipitin band formation to anti-IBDV specific serum. 28-day-old chicks inoculated with the isolate showed disease,and the isolate was recovered from the tissues of these chickens. The 1 041bp specific fragment were amplified by RT-PCR with sequence-specific primers based on IBDV VP3 gene. The VP3 gene shared 97.7%~98.2% nucleotide identities to very virulent IBDV,97.7%~95.2% nucleotide identities with classical strains from seqiemce analysis.

结果表明，该分离病毒无血凝性，在琼脂扩散试验中能与抗IBDV特异性血清出现1条清晰的白色沉淀线；人工感染28日龄雏鸡出现与临床一致的病变，并回收到病毒；应用针对IBDVVP3基因的特异性引物进行RT-PCR，能扩增到长度为1041bp的特异性目的片段；序列分析发现分离毒VP3基因与IBDV超强毒和经典毒株的核苷酸同源性分别为97.7%~98.2%和95.2%。

The difference as regards the latency and duration between EAP and EAU seems to show that the pineal gland is more susceptible to retinal autoimmune reac- tions than retina and uvea, indicating that one mechanism or factor for"ending inflammation"presents in ocular tissue rather than in pineal glands.

特异性抗体出现于EAU发生之前，EAU发生于特异性抗体上升阶段，提示体液免疫反应在其发生中起着一定的作用，而特异性Th细胞的激活及其所产生的IL-2，则可能对EAU的发病有更为重要的作用。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

Abstract］ Objective To evaluate the role of PSA,PSAD（prostate specific antigen density）,FPSA and F/T prostate specific antigen percentage in the diagnosis at the prostate cancer.Methods Levels of PSA,PSAD and FPSA were examined in serums of patients with prostate cancer（56 cases） and bisectional prostate hypertrophy（149 cases）.The comparison analysis were carried to these values.

目的 评价列腺特异性抗原、游离前列腺特异性抗原、游离/总前列腺抗原百分比及前列腺特异性抗原密度在前列腺癌鉴别诊断中的价值方法对56例前列腺癌和149例前列腺增生患者用微粒酶免疫法的血清总PSA、游离PSA及游离/总前列腺抗原百分比（F/T百分比；用经直肠超声检查测得前列腺体积V，计算出PSAD值对些值进行比较分析。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。