特异性

METHODS: Colonoscopic pictures from 300 HIV-negative patients (3000 pictures in total) and 32 HIV patients (600 pictures in total) with chronic diarrhea due to nonspecific colitis were comparatively analyzed. The pathological sections from the 32 HIV patients were observed for analyses of capillary vessel distributions and mucosal bleedings in lamina propria.

将同期不同症状和病因32例HIV非特异性慢性结肠炎腹泻患者600张电子结肠镜照片与300例非HIV患者3000张结肠镜照片对比，并对32例HIV非特异性慢性结肠炎腹泻患者病理切片内固有层内毛细血管分布情况和黏膜内出血情况进行分析。

METHODS: Colonoscopic pictures from 300 HIV-negative patients (3000 pictures in total) and 32 HIV patients (600 pictures in total) with chronic diarrhea due to nonspecific colitis were comparatively analyzed. The pathological sections from the 32 HIV patients were ob-served for analyses of capillary vessel distribu-tions and mucosal bleedings in lamina propria.

将同期不同症状和病因32例HIV非特异性慢性结肠炎腹泻患者600张电子结肠镜照片与300例非HIV患者3000张结肠镜照片对比，并对32例HIV非特异性慢性结肠炎腹泻患者病理切片内固有层内毛细血管分布情况和黏膜内出血情况进行分析。

The ELISA assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of Aspergllius, while the other assay could only detect Aspergillus fumigatus of both clinical and environmental isolates.And no cross reaction with the cell culture of Penialllium marneffei and Candidas was observed with the two methods.

用mAbs-1建立的双抗体夹心ELISA法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mAbs-2)建立的双抗体夹心ELISA法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心ELISA法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

The primers and the detection programs have been used to detect the pathogens in the inoculated-diseased cucumber plants .The details are reported as follows: 1. By using the primers ITS1/ITS4, only Fusarium isolates could be amplified to produce a special fragment of 750bp. This product generated 480bp, 200bp and 100bp fragments after digested by restriction enzyme HinfⅠonly from the pathogen of Cucurbits Fusarium Wilt.

引物ITS1/ITS4只在镰孢属菌株上扩增到一条750bp分子片段，其他供试菌株无扩增条带产生，扩增产物经HinfⅠ酶解后，只有瓜类尖镰孢菌产生480bp、200bp和100bp 3条带，说明该PCR-RFLP程序对瓜类尖镰孢菌具有特异性；巢式PCR引物BIK1/BIK4和BIK2/BIK3只有在黄瓜尖镰孢菌菌株上扩增到一条800bp分子片段，其他菌株均无扩增条带产生，说明该巢式PCR程序对黄瓜尖镰孢菌具有特异性。

The results provide neuroimaging evidence for the existence of acupoint specificity. 2. the study on acupuncture of combined-acupoints. It was found that the cerebral areas activated by the acupuncture of combind-acupoints were not a simple combination of those activated by the acupuncture at single acpoint, even in some activation areas, the activation intensity is weaker than at a single acupoint, which implied the existance of inteaction between acupoints in the combined-acupoints acupuncture. 3.the effect of acupuncture durion. This study found that the cerebral responses to half minute and one minute acupuncture were the same as a whole. But the cerebral responses to the acupuncture of 3 minutes were clearly different from those of 1 minute.

针对针灸中的基本问题，我们主要开展了以下研究：1，穴位特异性问题，初步证明了穴位特异性的存在。2，组合穴位针刺的机制研究，发现组合穴位针刺所激活的脑区并不是单穴位针刺结果的简单叠加，说明组合穴位针刺时单穴位之间存在相互作用。3，针刺时间对针刺效果的影响，在针刺治疗时针刺时间的长短到底对针刺效果有什么影响还不清楚，我们的结果显示针刺半分钟和1分钟之间没有显著差异，但它们与针刺3分钟之间存在显著差异，说明针刺存在时间效应。4，针刺四关穴对老年痴呆病人治疗作用的研究。

Using the on-line sequence similarity searching tool Fasta3 on internet web,many neuropeptides from crustacean CHH family of high similarity with thesededuced amino acid sequences from cDNA of eyestalks from these four kinds animalswere found.

在所有的相似序列中，分别由中国对虾、中华绒螯蟹、鹰爪虾和蓝对虾的特异性cDNA片段推定的氨基酸序列与蜕皮抑制激素的相似度最高，这一结果提示分别由中国对虾、中华绒螯蟹、鹰爪虾和蓝对虾的眼柄特异性cDNA片段推定的氨基酸序列可能是这四种甲壳动物的MIH片段。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

female Lewis rats with 6-8 weeks of age and 160-180g of weight were immunized subcutaneously into both hind footpads with 50μg Torpedo AChR emulsified in complete Freud's adjuvant in a total volume of 200μl and divided into 4 groups at random.

ELISA法动态观察外周血抗AChR抗体IgG含量以及特异性抗体亲和力的改变。3.ELISA法测定致敏后第45日，大鼠血清中AChR特异性IgG抗体亚型IgG1、IgG2a、IgG2b含量的改变。4。

The typical spot symptoms on fruit would decrease the production and merchandise of tomato in the heavy rain of June and July; therefore, the key period for disease control is after rainy season. Quick identification on pathogen was using PCR. 1476bp band was amplified by primer P3、P4of bacterial speck. By using primer CMM5、CMM6 for bacterial canker, 614bp fragment was amplified. These PCR tests provided important foundation for pathogen identification and disease diagnoses. Using primer RST65 and RST69 for bacterial spot, expected 420bp band was not amplified.

通过聚合酶链式反应对病原进行快速鉴定，证明加工番茄细菌性斑点病用引物P3、P4可以得到一条大小为1476bp的特异性条带，番茄细菌性溃疡用CMM5、CMM6可以扩增出一条大小为614bp的特异性条带，这为病害的快速诊断打下了基础，但利用引物RST65和RST69对番茄细菌性疮痂病进行鉴定，没有得到预期420bp的目标条带，故对细菌性疮痂病的快速检测还有待继续研究。

According to the specificity and sensitivity after different high-pass filters.

根据不同高通滤波频率后VLP各参数的敏感性及特异性的研究，表明40Hz高通滤波后其敏感性(66％)、特异性(96％)相对较高。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐