特异性

The sensitivity, specificity and Youden's index of PCR were 88.9%, 90.9% and 0.798 respectively when blood culture and five non-specific tests were used as a reference method.

若以血培养加非特异性指标为诊断标准，PCR法的灵敏度为88.9%，特异性为90.9%，正确诊断指数达到0.798。

Our study demonstrated that the epitope-specific CTLs function was defected. Interestingly, in contrast to previous study, our result found that direct ICCS assys using peptide core 18-27 were able to visulize a population of IFN-γin the absence of specific pentamer binding in patients.

值得注意的是，我们发现在慢性乙肝患者外周血中Treg频率明显增高且与表位特异性CTL的频率呈现负相关，这种现象给了我们新的提示，为此我们用磁珠分选出Treg后在体外作不同细胞亚群共孵育，从而研究Treg与表位特异性CTL的相互作用机制。

Methods After treated with a specific demethylating agent,Aza and acetylating agent, TSA, the status of 5'CpC island methylation of ING1b gene in HT29 human colon cancer cell line was analyzed using methylation specific polymerase chain reaction,and the level of histone acetylation was analyzed by chromatin immunoprecipitation,and reverse transcription polymerase chain reactionwas used to examine ING1b mRNA expression.

应用特异性DNA甲基转移酶抑制剂5-氮-2'-脱氧胞苷（5-Aza-2'-dc，以下简称Aza）及组蛋白去乙酰化酶抑制剂曲古抑菌素A作用人结肠癌细胞株HT29后，用甲基化特异性PCR检测该ING1b基因核心启动子区域CpG岛甲基化情况，用染色质免疫沉淀检测其乙酰化组蛋白绑定的DNA情况，并用逆转录聚合酶链反应检测ING1bmRNA表达。

Our results suggested that Onconase could increase its specificity via conjugating with ligands. Our studies also give the way to develop the new antitumor drugs.

我们的研究证明了构建基于Onconase的特异性抗肿瘤蛋白的可行性，为以后开发Onconase的特异性抗肿瘤药物打下了基础。

Methods: Based on unique sequence characteristic of the hilA gene in Salmonella, the bacterium was amplified on Salmonella by PCR.

设计沙门菌hilA基因的特异性引物，优化PCR反应条件，对沙门菌和非沙门菌进行特异性引物的PCR扩增，鉴定沙门菌。

Methods Three pairs of primers have been designed according to the hilA of Salmonella spp., hemolysin of Proteus mirabilis and Staphylococcus aureus clone pSa-442 Sau3AI fragment and the anticipated PCR products were 580bp, 401bp and 256bp.

本研究依据沙门菌侵袭基因正调节蛋白基因、变形杆菌溶血素基因和金黄色葡萄球菌特异性pSa-442序列，运用Primer Premier 5.0分别设计3对特异性引物，预计PCR扩增的目的基因片段分别为为580bp、401 bp、256 bp。

Both cTnT and cTnI are more useful for stratifying risk in acute coronary syndrome patients than CK-MB.

在症状发生后的12小时采样分析cTnT，诊断MI的临床灵敏度和特异性分别是98.2%和8.8%，临床特异性降低与检测到微小心肌受损有关。cTnT和cTnI比CK-MB预测急性冠状动脉综合症危险性更有用。

As a result,the OmpK genes not only were distributed widely in all tested Vibrios,but also shared quite high similarity in nucleotide sequences and deduced amino acid sequences.

序列分析还表明，每一种弧菌OmpK基因都有一段特异性序列，可用于设计核酸探针或特异性引物来诊断、检测哈维氏弧菌等海水鱼致病性弧菌。

The Scatchard analysis of data revealed a linear relationship between the amount of specific binding and the ratio of specific binding to free 125 I (D Lys 6)LHRH, indicating a single class of high affinity sites. Equilibrium dissociation constant was 0 34 nM in pituitary membrane preparation and 0 43nM in isolated pituitary cells.

特异性结合随125 I( DLys6 ) LHRH 量的增加而增加，实验结果经Scatchard 分析揭示出特异性结合与结合和游离125I( DLys6)LHRH 之比呈线性关系，预示绍鸭垂体对( DLys6)LHRH 存在同一类高亲和力的结合位点。

It was shown that with parallel detection of specific and non-specific recognitions, the accuracy of bioassay would be effectively enhanced.

实验结果显示，同时检测特异性和非特异性的识别，可以有效提高生物检测的准确性。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐