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Despite evidence implicating prostaglandins as critical factor in male reproductive functions, little is known about expression and function of COX-2 in the male reproductive system. By using immunohistochemistry, RT-PCR and Western-blot, the content of this research included:(1) The expression and localization of COX-2 in mature rat and human testis were examined.(2) We investigated the changes of spermatogenesis and testosterone levels in testis after by using the COX-2 specific inhibitor-rofecoxib at 2, 4 and 8 weeks.(3) To explore the relationship between the intratesticular level of testosterone and expression of COX-2 by completely destroying the Leydig cells of mature male rats with injection of a single I.

本课题应用免疫组织化学、RT-PCR和Western-blot等方法检测以下内容:(1)在mRNA和蛋白水平检测正常成年男性及大鼠睾丸组织中COX-2的表达及定位情况;(2)实验组大鼠持续给予COX-2酶的特异性抑制剂Rofecoxib,分别于用药后2周、4周和8周检测大鼠的精子生成以及睾丸组织内睾酮浓度的变化;(3)另一组实验大鼠给予特异性Leydig细胞灭活剂EDS,在特定时间内抑制睾丸组织内合成的睾酮,于用药后2周和4周,采用免疫组织化学及RT-PCR方法观察大鼠睾丸组织内睾酮浓度变化对COX-2合成的影响。

Objective: Identify the human embryonic germ cells by observing the expression of its specific surface markers SSEA-1、SSEA-3. Methods: Human embryonic genital ridges, dorsal mesenteries(5-9 weeks post-fertilization) were cultured without any cytokines, while the fibroblasts derived from the isogenous embryo were used as the feeder layer.

目的:组织块培养法培养人胚胎生殖干细胞,通过检测细胞所表达的阶段特异性胚胎抗原1 和3(stage-specific embryonic antigen-1、3 ,SSEA-1、SSEA-3),对未分化的人EG 细胞进行特异性的生物学鉴定。

aeroallergen specific ige antibody in the serum of 625 patients with allergic rhinitis were positive. total ige in serum was positive in 413 cases, accounting for 66.1%. the positive rate of total ige was less than that of aeroallergen specific ige. the positive rate of aeroallergen specific ige in order were mite mixture (84.32%), cockroach (19.04%), cypress (15.20%), cat-dog epithelia (13.12%), tree mixture (7.20%), ragweed (5.12%), humulus lupulus (1.76%), mugwort (1.76%), mould mixture (1.44%). in the two groups, the positive rate of aeroallergen specific ige in order were similar.

625例变应性鼻炎患者中吸入性变应原特异性ige阳性率依次为尘螨84.32%、蟑螂19.04%、柏树15.20%、动物毛皮屑组合13.12%、树木花粉组合7.20%、矮豚草5.12%,葎草1.76%、蒿草1.76%、霉菌组合1.44%;总ige阳性者413例(66.1%)明显低于特异性ige阳性率;两组中最常见的变应原相似;未成年组屋尘螨的阳性率及敏感程度明显高于成年组(p 0.01)。

Therefore the nonrandom distribution of 3′ end sequences of effectual primers in tomato RAPD reflected the sequence-specific structure may occur in tomato genome at flank of polymorphic regions. It is probably an attribute of tomato genomic sequence and is common in Lycopersicon.

RAPD引物3′端序列的非随机分布,反映了番茄基因组内的多态性区段侧翼具有序列的特异性,这种特异性结构应是番茄基因组序列的一种属性,这种属性为番茄属基因组所共有。

WB-DWI is a sensitive, but less specific method for diagnosis of lymphoma. It is difficult to differentiate lymphomatous from metastatic lymph nodes. ADC value may provide help in diagnosis of lymph nodes malignancy.

WB-DWI诊断恶性淋巴瘤的敏感性高,而特异性不强,难以与原发灶不明的淋巴结转移癌相鉴别,ADC值有一定帮助但不具有特异性

Comparing with diagnoses in follow-up, PET-CT imaging had a sensitivity of 85.7% and a specificity of 87.5% in diagnosis of recurrence in the same place of the primary focus; a sensitivity of 88.9% and a specificity of 85.7% in diagnosis of lymphous metastasis.

与随访结果比较PET-CT对鼻咽癌原位复发诊断灵敏度85.5%,特异性87.5%;对鼻咽癌淋巴结转移的诊断灵敏度88.9%,特异性85.7%。

There is usually little or no progression, the EMG shows non-specific myopathic features and serum CK is normal or only slightly raised.1 The muscle biopsy may show characteristic ultrastructural abnormalities but these can be difficult to detect without specific histochemistry and/or electron microscopy that were not available in 1981 when our patient was biopsied.

经常表现为缓慢或者无明显进展,EMG提示非特异性肌源性改变特征,血清CK是正常的或者仅仅轻微的升高。肌肉活检可能表现为特征性的超微结构异常,但是这些改变必须通过特异性的组织化学和/或电镜才能观察。而在患者进行活检时的1981年这些手段是达不到的。

Sensitiity and specificity were 73%(95% CI 60-86) and 91%(79-100) for neuromyelitis optica and 58%(30-86) and 100%(66-100) for optic-spinal multiple sclerosis. NMO-IgG was detected in half of patients with high-risk syndromes.

视神经脊髓炎的敏感性和特异性分别为73%和91%,视神经-脊髓型多法性硬化的敏感性和特异性则分别为58%和100%。

Joint, occipitoatloid and post-atlantal-arcuate we consider- ed that the anatomic position exposed in each of the ten postures has both its generality and specificity, and only when a specific posture is apphed to its aim, or a general one chosen according to clinical...

认为各种体位显示解剖位置有其共性和特异性特异性的体位应用应有针对性,有共性的体位应结合临床综合优选应用,方能对颈部骨伤科疾患更加准确、迅速、有效的作出X线诊断。

CaB participated in the human odontogen esis. Besides, the express of CaB in human toot h germ development was time-specific and cell-specific.

CaB参与了人釉质及牙本质的形成过程,并且在人牙胚发育过程中的CaB表达具有时间特异性及细胞特异性

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

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