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To define further the fragments regulating gene expression, the Lcn5 promoter was examined in transgenic mice and immortalized epididymal cell lines.

经过一系列的缺失试验后,发现在转基因小鼠中Lcn5基因1.8 kb长的启动子片断对于基因的组织特异性和区域特异性表达是必需的。

The results showed that all the strains of A. avenae subsp.citrulli tested produced 360bp specific fragments by the ISE and the direct PCR method, while others including 23 strains of Pseudomonas, Xanthomonas, Erwinia, Clavibacter, Burkholderia and Bacillus showed negative PCR result.

用直接PCR技术和免疫捕捉PCR技术检测西瓜果斑病菌,结果表明,所有果斑病菌都能产生360bp左右的特异性片段,而对照Pseudomonas、Xanthomonas、Erwinia、Clavibacter、Burkholderia及Bacillus的23菌株无特异性片段产生。

Results: The sensitivity and specificity of CEA and CYFRA21-1 in differential diagnosis of pleural effusion were 81%, 56%, and 64%, 95% respectively, in combination with the exfoliative cytology examination of pleural effusion and immunofluorescence technique, the sensitivity and sepcificity were increased to 100%.

结果:CEA、CYFRA21-1对胸腔积液诊断的敏感性及特异性分别为81%、56%和64%、95%,联合检测胸水脱落细胞、免疫荧光法检测肿瘤细胞,其特异性和敏感性均提高到100%。

PART 2:The molecular mechanisms of Nontypeable Haemophilus influenzae induced inflammatory response in respiratory epithelial cells Objective:Nontypeable Haemophilus influenzaewas traditionally considered as an extracellular pathogen in respiratory infections.In this study,we established a model of infected cells to confirm NTHI was also a facultatively intracellular pathogen.Mitogen activated protein kinasesare the important signal transduction pathway in mediating inflammatory response induced by NTHI in respiratory epithelium.In this study,we elucidate the impact of NTHI,and its SCF and EP on the expression of MAPK and subsequent proinflammatory mediators.

第二部分:NTHI致呼吸道上皮细胞炎症反应的分子机制研究目的:通过建立呼吸道上皮细胞株的体外NTHI感染模型,确认NTHI在细胞内的存活,观察NTHI及其组分(可溶性胞质成分SCF、外膜蛋白EP)对呼吸道上皮细胞MAPK信号传导通路和后续的前炎症因子表达的影响,并应用特异性ERK-MAPK抑制剂U0126、p38 MAPK抑制剂SB203580特异性阻断下游MAPK信号传导通路,观察它们对呼吸道上皮细胞分泌前炎症因子表达的影响。

The re-suits showed that IL level in serum of Ⅰ group goats decreased, whereas that of Ⅱ group goats increased, TNF-α levels in serum and IL-2 in supernatant with proliferation of PBL of goats increased after infection. PBL of goats was responsible for antigen 1 week after infection. Immune response of PBL of goats infected with Fasciola hepatica on the special and non-special antigen obviously increased. The results suggested that TNF-α would participate in the course of liver damage caused by Fasciola hepatica.

结果表明,山羊试验感染肝片吸虫后,Ⅰ组山羊血清IL-2水平在感染后有所下降,而Ⅱ组山羊血清IL-2水平在感染后有所升高;感染不同剂量肝片吸虫的山羊血清TNF-α水平和淋巴细胞IL-2分泌水平均有不同程度的升高,在感染后第1周淋巴细胞就有反应,并且对非特异性刺激原和特异性刺激原的应答都有显著升高。

For Streptococcus penumoniae、Hemophilus in0fluenzae、Staphylococcus aureus、Acinetobacter baumannii、Pseudomonas aeruginosa,the sensitivety of Tem-PCR was 100%、41.7 %、11.1%、50.0 %、66.7 % respectively; the specificity was 84.0%、81.7%、98.9%、95.2%、98.9% respectively. Tem-PCR was more sensitive than culture in the detection of Streptococcus penumoniae 36/19.1% vs.

3以细菌培养作为参考标准,Tem-PCR对14种病原菌总的敏感性为51.0%,特异性为68.0%,符合率为58.3%,对肺炎链球菌、流感嗜血杆菌、金黄色葡萄球菌、鲍曼氏不动杆菌、铜绿假单胞菌的敏感性分别为100%、41.7%、11.1%、50.0%、66.7%,特异性分别为84.0%、81.7%、98.9%、95.2%、98.9%。

The sensitivity, specificity, PPV, and NPV of ECE for BE patients undergoing surveillance were 79%, 78%, 94%, and 44%, respectively. The sensitivity, specificity, PPV, and NPV for erosive esophagitis were 50%, 90%, 56%, and 88% and for hiatal hernia were 54%, 67%, 83%, and 33%, respectively.

腐蚀性食管炎的ECE相对于BE的灵敏度,特异性,阳性预测值以及阴性预测值分别为50%, 90%, 56%,和88%,食道裂孔疝的ECE相对于BE的灵敏度,特异性,阳性预测值以及阴性预测值分别为54%, 67%, 83%,和 33%。

During embryonic development, Hox proteins can interact with different cofactors and regulate downstream gene expression in a tissue specific manner.

在胚胎发育过程中,Hox蛋白实现时间特异性及组织特异性基因表达调控功能的机制之一是与不同的辅助因子相结合。

In this study, oral tolerance was induced by tube-feeding transgenic mice with 0.5mg/day OVA protein daily, simultaneously sensitized by OVA protein with aluminum hydroxide. Oral-administered OVA protein (0.5mg/day for 5 or 10 consecutive days) resulted in significant lowered airway hypersensitivity as well as reduced inflammative cells infiltration, titer of serum OVA-specific IgE, IL-5 secretion in bronchoalveolar lavage fluid, and IL-4 secretion in splenocyte.

首先,本实验於小鼠中成功诱发了呼吸道过敏、大量OVA特异性IgE抗体生成、及嗜酸性白血球不正常聚集等现象;致敏前,同以连续管餵小鼠OVA抗原(0.5mg/day) 5天或10天后,结果显示皆能缓和呼吸道阻力、减低肺部发炎细胞浸润现象、降低血清中OVA特异性IgE抗体的生成量、也减少肺部冲洗液及脾脏细胞内第二型辅助T细胞(type II helper T cell, Th2)细胞激素的分泌(IL-4、IL-5)。

Methods: The serum specific ingestive and inhalant allergens of 586 cases of urticaria and urticaria papulosa patients were detected by allergen in vitro test kit.

采用过敏原特异性IgE抗体检测试剂盒对586例吸入组及食物组急、慢性荨麻疹及丘疹性荨麻疹患者血清进行过敏原特异性IgE抗体检测。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。