特异性

For detecting rotator cuff tear , the sensitivity , specificity , and accuracy of MR arthrography were 100 %, 100 %, 100 % respectively. For detecting complete rotator cuff tear , the sensitivity , specificity , and accuracy of MR arthrography were 100 %, 94 %, 97 % respectively.

肩关节MR 造影判定有无肩袖撕裂的敏感性、特异性和准确性都为100 %；诊断全层撕裂的敏感性、特异性和准确性分别为100 %、94 %和97 %。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

Fragments from the 239th to the 944th nucleotide of the coding regions of all actin mRNAs were amplified with two actin specific primers. Fragments from the 239th nucleotide of the coding region to the end of actin mRNAs were also amplified The electrophoresis results were compared between the macropterous and brachypterous brown planthoppers.

利用两条肌动蛋白特异性引物，扩增出了肌动蛋白基因mRNA的编码区片段，另外，将肌动蛋白特异性引物作为上游引物，将与3'锚定反转录引物部分序列相同的引物作为下游引物，扩增出了从肌动蛋白基因mRNA的编码区第239号碱基到Poly尾的这段cDNA，并在翅型之间作了比较。

It is concluded that both inasie and noninasie pulmonary function tests are capable of detecting allergen-specific as well as non-allergic bronchoconstriction in intact mice or rats.

结论是：无论是侵入性还是非侵入性肺功能实验，在大鼠或小鼠体内，都能检测出特异性变应原和非特异性变应原引起的支气管收缩。

Doetschman TC, Eistetter H, Kemler R, et al. The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium ［J］. J Embryol Exp Morph, 1985,87:27～45.［2］ Robbins J, Doetschman T, Jones WK, et al. Embryonic stem cell as a model for cardiogenesis［J］. Trends Cardiovasc Med, 1992,2:44～50.［3］ Moreadith RW, Radford NB.

被诱导的心肌细胞的判定除常规指标外，我们还用了心脏特异性引物β-MHC对不同收缩时间的心肌细胞总RNA进行RT-PCR扩增和RNA斑点杂交，发现该心肌细胞不仅具有正常心肌细胞的特性，而且在分化早期就高度地表达心脏特异性基因。

The established method, with higher specificity and reproducibility, could accurately differentiate genuine antler from its adulterants and would be widely used in Cervus related Chinese crude drugs′ identification.

所建立的位点特异性PCR方法，能将正品鹿茸与其他近源种鹿茸药材鉴别开来，具有较高的特异性、重复性，可广泛应用于鹿类药材的鉴别。

The established method, with higher specificity and reproducibility, could accurately differentiate genuine antler from its adulterants and would widely used in Cervus related Chinese crude drugs' identification.

所建立的位点特异性PCR方法，能将正品鹿茸与其它近源种鹿茸药材鉴别开来，具有较高的特异性、重复性，可广泛应用于鹿类药材的鉴别。

However takes one kind of precious plants,not actually have the reseach for the chimonanthus praecox in molecular biology.

在分子生物学技术不断取得前进的前提下，人们发现在植物的花药发育和花粉成熟过程中，有大量的基因表达，其中有许多是花药和花粉组织特异性的，目前花药和花粉特异性表达的基因及其调控序列已经成为研究的热点。

Methodological design, synthesis is aimed at anoxic and revulsive factor - the nucleotide of few of particularity short cable length of 1 α is acerbity, compose builds anoxic and revulsive factor - of ShR-NA of 1 α particularity recombine plasmid, stability turns catch; of cell of colonic cancer SW 480 to use chloridize cobalt preparation is anoxic and revulsive education radical, imitate tumour is anoxic condition.

方法设计、合成针对缺氧诱导因子-1α的特异性短链寡核苷酸，构建缺氧诱导因子-1α特异性shR-NA的重组质粒，稳定转染结肠癌SW 480细胞；采用氯化钴制备缺氧诱导培养基，模拟肿瘤缺氧状态。

Through analyzing the driving energy of intercalation and the force of sequence special recognition, it can be concluded that both the intercalation and special recognition are mainly through electrostatical interaction.

对溴化乙锭插入DNA分子的驱动力和序列特异性识别的作用力分析，揭示溴化乙锭插入DNA分子的驱动力和碱基对的特异性识别均以静电作用为主。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。