物

The fragment size can be amplified by 295 bp through the primer, in order to prevent false positive from appearing, a pair of internal control primers C34 is designed through the invention according to a bull autosome 3 reported sequence, the fragment size is amplified by 208bp, dual PCR amplification is performed to single bull sperm through the two pairs of primers, and then the final evaluation is performed to the sperm separation purity according to the statistical analysis to the detection result.

本发明提供了一种检测X、Y精子分离纯度的引物，该引物是针对牛Y染色体上性别决定基因Sry通过PCR错配技术设计而成，通过该引物可扩增片段大小为295bp，为了防止假阳性出现，本发明根据牛3号常染色体报道序列设计了一对内标引物C34，扩增片段大小为208bp，通过上述两对引物对单个牛精子进行双重PCR扩增，然后根据对检测结果的统计分析，对分离精子纯度做出最终评价。

objective comparison of 120 example cervical wecretion directly smear and culture.methods one sterile swab directly smear;gram stain;bacterioscopy, others swabs inoculate chocolate plate;blood plate;shaborose plate;culturing 24 hours then identification.results ng and bv directly smear isolates rate higher than culture;butfung culture isolate rate higher than direct smear to bacterioscopy.conclusion cervical wecretion directly smear to bacterioscapy has rapidly and simply diagnostic value

摘 要］目的：比较120例妇科宫颈分泌物直接涂片镜检与培养结果的分析。方法：宫颈分泌物直接涂片、革兰染色、显微镜检与宫颈分泌物接种于巧克力板、血平板、沙氏板进行培养和鉴定。结果：对分泌物中的致病菌而言，g-双球菌和阴道加德纳菌的直接涂片镜检阳性率较培养高；霉菌的培养阳性率较直接涂片镜检率高。结论：妇科宫颈分泌物直接涂片镜检具有快速、直观、简便的诊断价值。

Something something something something something camel cattle car cattle Tuoma slaves estate Shezhai four right-field Township □ channels and pieces of family property to ...

某物某物某物某物某物车牛羊驼马驼畜奴婢庄园舍宅田地乡□渠道四至右件家产并以。。。

As a consequence, it accelerated the processes of biogeochemical cycling of nutrient in the Yangtze estuarine and tidal flat sediments. Corbicula fluminea mainly inhabited near the surface tidal sediment. It affected the nitrogen cycling in the surface sediments by the physiological activities such as the excretion.

蟹类动物通过生物扰动改变了沉积物的物理结构，增加了沉积物与大气之间的接触面积，使沉积物氧含量大为增加，加快了沉积物中生源要素赋存形态之间的转化，因此蟹类活动促进了潮滩沉积物中生源要素的生物地球化学循环过程速率。

Its bonding characterization and stability are discussed. The results show that these complexes are covalence complexes and that both the in-plane σ-bond and the in-plane π-bond in these complexes play an important role. The ESR spectra of VO -Ser system, VO -Ser-Phen system and VO -Ser-Bipy system in binary solvent glycol/water (V/V=1: 1) solution at various acidities (pH=1～14) have been observed at low temperature. It is found that in different pH ranges different structural complexes are formed.

测定了五种氨基酸席夫碱铜配合物的室温多晶粉末ESR谱、在不同溶剂中的室温及低温溶液ESR谱，确定配合物的配位环境；对室温溶液谱中观察到的二级效应和驰豫效应给予了理论解释、计算了驰豫参数；根据低温ESR波谱，计算了配合物的成键特性及稳定性，发现此类配合物为共价型配合物，平面内σ配键和平面内π配键均起重要作用。

The novel primers were evaluated by detecting BTV serotypes 1, 3, 5, 8, 10, 11, 21 and 22. The specificity of the primers was estimated by comparing to gene sequences of viruses published in GenBank, and further assessed by detecting BTV serotype 1-12 and Epizootic hemorrhagic disease virus serotype 1-4. The sensitivity and repeatability of PCR with the novel primers were evaluated by successfully detecting the recombinant plasmid pGEM-T121 containing the diagnosed nucleotide sequence.

Blast软件分析引物与GenBank发表的病毒基因的同源性，显示引物特异性较好；利用8个不同血清型BTV标准毒株进行RT-PCR检测，证实该引物能有效检测不同血清型BTV；通过BTV1-12和EHDV1-4进行检测，证实该引物具有较好的检测特异性；通过制备的含特检序列的质粒标准品P121进行检测，显示该引物检测的敏感性为105 copies，重复检测CV%为0；对模拟血清样品进行检测，结果均为阳性。

The mass loss patterns of four type litters fitted exponential model, and the decomposition rate could be well predicted by their lignin/N ratio.

结果表明：与对照相比，降水量减少条件下，蒙古栎叶凋落物的初始N、P、K浓度显著升高，初始木质素浓度显著降低，凋落物分解速率大，N、P、K矿化率高，N和P固持时间缩短；降水量增加情况下，其凋落物初始N浓度显著降低、木质素浓度显著升高，N、P、K矿化率低，N和P固持时间延长。4种类型叶片凋落物的质量损失过程均符合指数降解模型，分解速率可以由凋落物木质素／N来预测。

objective to compare four postoperative nasal packing materials.methods total 136 patients,80 cases with chronic sinus it is and under went endoscopic sinussurgery,were randomly packed using four different hae mostasis materials.in which,paraffin gauze group was 40 cases,rapid sorbalgon group 27 cases,merocel group 36 cases and gel knit group 31 cases.the packing materials wereemoved after left for 24 hours to 48 hours.the efficacy of nasal packing materials was asassessed interm of the levels of nasal pain or headache in the leaving period and nasal bleeding after removal of nasal packing.results the in cidence rate of nasal pain or headache respectively was 82.5% in paraffin gauze group,44.4% in merocel,11.1% in sorbalgon and 3.2% in gel knit.the significant statistical difference was observedbetween the four groups (χ2=70.21,p.01).the incidence rate of nasal bleeding after removed was 85%,11.1%,41.7% and 9.7% respectively.the statistical difference was also significant (χ2=54.28,p.01).conclusions the choosing of postoperative na sal packing after functional endoscopic sinus sur gey depends on various factors,gel knit has much advantages and can be used as routine packs after fess.

摘 要］目的：比较四种鼻腔填塞材料的疗效，指导临床选择合适的术后鼻内填塞物。方法：对134例慢性鼻窦炎分别采用凡士林纱条(40例)、藻酸钙纤维(sorbalgon，27例)、膨胀海绵(merocel，36例)和瑞纳凝胶快速止血材料(gel knit，31例)四种材料填塞鼻腔，24 h～48 h取出填塞物，根据填塞后鼻腔胀痛、头痛程度，取出填塞物后鼻腔出血程度等评价疗效。结果：凡士林纱条填塞组，82.5%有鼻腔胀痛或头痛；sorbalgon藻酸钙纤维组11.1%；merocel组44.4%；gel knit组3.2%，四组比较差异非常显著(χ2=70.21，p.01)。取出填塞物后鼻腔再出血分别为85%，11.1%，41.7%和9.7%，四组比较差异非常显著(χ2=54.28，p.01)。结论：鼻内镜手术时，应综合考虑诸方面因素合理选用术后填塞物。瑞纳优势较为全面，可作为鼻内镜手术的常规填塞材料。

The method of self-consistent field of the intermediate neglect of differential overlaphas been used in the calculations of the binuclear cluster, trinuclear unit of hexanuclear clusterand octanuclear clusters. The results are described as follows: The high-lying MO's of thetrinunclear and binuclear cluster are mostly composed of d-orbitals of the metal cores, and areconsidered as M-M bonding orbitals.

应用INDO／S方法对二核簇合物、六核簇合物中的三核片断和八核簇合物进行了计算，结果表明，二核簇合物与三核簇合物的前线占有轨道主要是由金属簇芯的d轨道组成，是M-M成键轨道。

With regard to the scope of subject matter of lien, it includes not only personal property, but also real property and valuable instruments which possess characteristicics of personal property; contains not noly merchantable things, but also prohibited merchantable things and limited merchantable things, chapter III is about question of deadiline of lien, lien isnt invalid as prescription of quaranteed obligatory right completes ,but lienor possesses the fruits odf subject matter of lien, because of his occupying cubject matter of lien, because of his occapying subject matter of lien.therefore lien is different from mortgage, is without excluded period.

然后，第二章，针对我国留置权适用范围、留置物范围限缩的现实，提出了留置权不仅即适用除原规定的几类合同〔加工、运输、保管、行纪上，也应适用于无因管理等准合同关系中；而在留置物范围上，即应包括动产，也应当包括不动产和具有动产特定的有价证券；即包括流通物，也包括不可流通物与限制流通物。第三章是关于留置权时效、期限的问题。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。