牛结核病

In order to develop an indirect ELISA for diagnosing bovine paratuberculosis, the DNA fragmentsof map086 2and map2154c were amplified from the genome DNA Mycobacterium avium Subspeciesparatuberculosis K10. Then the DNA fragments of map0862 and map2154c were spliced byoverlapping extension, yielding a fusion gene map0

为了建立一种有效的牛副结核病间接ELISA方法，本研究设计了两对引物，从副结核分枝杆菌P_(18)株的基因组中PCR扩增出两个目的基因map0862和map2154c、，采用重叠延伸剪接技术(splice by overlapping extension，SOE)获得融合基因map0

More than 10% of human tuberculosis is caused by M. bovis.

人结核病的10％以上是由牛分枝杆菌引起的。

Bovis has been most often documented in Hispanic communities with close proximity to Mexico, the researchers point out that a recent review of such cases in New York City – also linked to unpasteurized cheese from Mexico – indicated that the tuberculosis problem is not limited to US regions bordering Mexico.

而米牛已最常记录在西语裔社区与邻近的墨西哥，研究人员指出，最近检讨这种情况下，在纽约市-也与未经奶酪由墨西哥-指出，结核病的问题并不限于美国地区接壤的墨西哥。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。