牛结核病

Western blot analysis shows that the protein could be identified only by the antiserum to M. bovis but not the sera against.M, paratuberculosis, brucellosis, babesia and infectious bovine rhinotracheitis virus.

Western blot分析显示：该融合蛋白能与抗牛分枝杆菌阳性血清发生特异性反应，而与牛副结核病、牛布氏杆菌病、牛巴贝斯病和牛传染性鼻气管炎病阳性血清不反应。

" This substance, which he designated "TC", plays the important part in the immunizing action of Professor Behring's "bovivaccine, which prevents bovine tuberculosis .

这种物质，他被指定为"技术合作"，发挥的重要组成部分免疫行动中教授特贝林的" bovivaccine "，以防止牛结核病。

The research aimed to provide references for the DNA vaccine development of bovine tuberculosis.

［目的］为牛结核病DNA疫苗的研制提供参考。

Mycobacterium bovis is the causative agent of bovine tuberculosis in a range of animal species and human beings.

牛结核病主要是由牛分枝杆菌引起的一种人兽共患传染病。

The gene of ESAT-6 from genome of bovine mycobacterium tuberculosis strain that from cervine tuberculosis was amplified by polymerase chain reaction and inserted into pGEM-TE vector. After sequencing, we analyzed the gene encoding ESAT-6 protein with molecular biologic software.

从患结核病鹿的肺组织中粗分离牛分支杆菌制备DNA模板，用PCR对牛分支杆菌ESAT-6基因进行了扩增，将扩增的产物连接于测序载体pGEM-TE上克隆，经测序确定无误；并使用分子生物学软件对ESAT-6基因进行了分析。

Sera from 90 cattle with bovine paratuberculosis and 100 cattle without bovine paratuberculosiswere tested.

通过对90份牛副结核病血清和100份无副结核病牛血清的检测结果表明，ELISA方法的敏感性为84.44％(76／90)，特异性为97％(97／100)。

TheDNA vaccine can effectively stimulate the organism to produce persistent humoral immunity andcellular immunologic response. Meanwhile, using a DNA vaccine dose not interfere with the allergydetection of bovine paratuberculosis and bovine tuberculosis.

由于DNA疫苗能有效地刺激机体产生持久的体液免疫和细胞免疫应答，同时，DNA疫苗免疫后，不干扰副结核病和牛结核病的变态反应检测。

The protective efficacy of the fusion DNA vaccines was surpass the polyvalent DNA vaccines and equivalent to that of the BCG vaccine, suggesting that fusion DNA vaccines represent a promising approach for the prevention of BT. So our experiment may be useful for the development of Bovis tuberculosis vaccine.

从攻毒保护效果来看，融合DNA疫苗组的保护效果明显优于多价DNA疫苗，达到了BCG疫苗的免疫保护水平，表明本研究制备的融合DNA疫苗具有良好的用前景，从而为牛结核病新型疫苗的研究奠定了基础。

In this study,bovine serum albumin was used as components of blocking buffer,diluents for serum and enzyme-labeled secondary antibody,respectively.

牛结核病是一种人兽共患传染病，不仅严重影响养牛业的健康发展，而且由于人类与奶牛间的密切关系，也同时严重威胁着人类的健康。

Based on the above research,we got that there was some antibody of bovine serum in BSA.

在国内外已有的报道文献中，BSA也是牛结核病ELISA检测

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。