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In this study, the classification of Chinese Babesia spp. infective to cattle were carried out by the classical methods and molecular biotechnology, and investigated the vector ticks of an unidentified Babesia sp. isolated from Xinjiang province by the experimental transmission, and observed the developmental forms of Babesia sp. in the engorged Hyalomma anatolicum anatolicum females;We developed the indirect hemoagglutination test for Babesia bigemina, and evaluated its effect in the sero-survey;We made slow-released injection of imidocarb, and estimated its efficacy on the treatment and prevention of Babesia bigemina infection by the laboratory work and the field tests. The major results were presented as following:1. Discovery of a bovine Babesia sp.

本研究以牛的巴贝斯虫为研究对象,采用传统技术与分子生物学技术相结合的方法对在我国已分离的牛巴贝斯虫病主要病原进行了分类学研究,在实验室内运用蜱传播试验对分离于我国新疆的牛的巴贝斯虫未定种的媒介蜱及其传播方式进行了详细的研究,采用组织抹片方法对牛的巴贝斯虫未定种在小亚璃眼蜱饱血雌虫体内的发育过程进行了观察;建立了间接血球凝集试验诊断方法,并对其在血清学调查中的应用效果进行了考察;研制了咪唑苯脲缓释注射剂,并通过实验室工作和田间试验对防治效果进行了评价。

Experimental transmission of an unnamed bovine Babesia by Hyalomma spp., Haemaphysalis longicornis and Boophilus microplus: Experiments were undertaken to determine the mode of transmission to cattle of an unnamed Babesia sp.

二。采用蜱传播试验,研究了璃眼蜱蜱种、长角血蜱和微小牛蜱对牛的巴贝斯虫未定种的传播能力与传播方式:为了确定小亚璃眼蜱、残缘璃眼蜱、麻点璃眼蜱、长巴贝斯虫未定种的发现及牛巴贝斯虫病的免疫诊断和药物防治研究角血蟀和微小牛蟀对在我国分离的牛的一种未命名的巴贝斯虫的传播能力与传播方式而进行了研究。

For this purpose, genotyping was performed on males of 18 Angus, 23 Jinnan cattle, 20 Limousin, 28 Luxi cattle, 26 Qinchuan cattle, 29 Simmental, 29 Charolais. In the association studies traits of interest were analyzed using the RFLP-PCR and GLM procedure of SAS 9.1 and least square means of the genotypes were compared by the Tukey' s test.

基于这一目标,应用RFLP技术对18头安格斯,23头晋南牛,20头利木赞,28头鲁西牛,26头秦川牛,29头西门塔尔牛和29头夏洛莱牛进行了基因型分型,SAS 9.1的GLM过程中的Duncan's 检验对不同基因型之间进行了多重比较分析,结果表明,EcoRⅤ位点与体重、日增重、眼肌面积和嫩度之间存在显著生物学相关,并且AG基因型个体与其它基因型相比在日增重和嫩度上存在显著差异(p0.05),而AA基因型个体相对于其它基因型具有较大的眼肌面积(p0.05)。

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术,对比研究正常与白化大菱鲆有眼侧皮肤组织,克隆差异表达基因,经测序,RT-PCR 验证,差异表达基因片段DEN-1(GenBank登录号:DQ886390)与DEN-2(GenBank登录号:DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达;通过BLAST检索发现,DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖:糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖:神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性;DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

Objective To evaluate the safety and effectiveness of protein-free calf blood extract eye gel for repairing corneal epithelial defect by multiple centers, double mask and randomized clinical study with parrel contrast of bFGF eye gel as positive control.

目的 以碱性成纤维细胞生长因子滴眼液为阳性对照药物,通过随机、双盲的多中心平行对照临床试验,评价小牛血去蛋白提取物眼凝胶治疗角膜上皮缺损的疗效和安全性。

The 16 cuts, according to their meat quality, were classified into 3 major characteristics groups: tenderloin, high rib, ribeye and striploin were classified as one group, belonging to high grade meet; fore beef shank, rear beef shank, beef flank, beef neck, knuckle, triangle meat and beef shoulder were classified into the second group; silverside, topside, beef rump, rib cut, and brisket, the third group.

聚类分析结果表明牛柳、上脑、眼肉、西冷4个部位的牛肉在品质上相似而聚为一类,属于高档牛肉;会牛扒、米龙、臀肉、肋条和胸肉则划归为第二类,属于中档牛肉;前腱、后腱、牛腩、牛前、和尚头、前柳和肩肉则聚为第三类,属于一般档次的牛肉。

Eight Qinchuan cattles(3 males,3 females and 2 steers) were slaughtered and carcass were divided up to 16 retail cuts: tenderloin, striploin, ribeye, high rib, brisket, rib cut, beef rump, topside, knuckle, silverside, fore beef shank, rear beef shank, triangle meet ,beef neck and beef shoulder, from which the following items, including content of water, crude ash, crude fat, crude protein, meat colour, pH value, tenderness and water holding capacity were measured.

本研究选取发育正常、健康无病、年龄在1.5岁的秦川牛公牛3头、母牛3头、阉牛2头共8头牛进行屠宰,对牛柳、西冷、眼肉、上脑、胸肉、肋条肉、臀肉、米龙、膝圆、黄瓜条、牛前腱、牛后腱、牛腩、牛前柳和牛前等16个部位取样进行水分、粗灰分、粗脂肪、粗蛋白、肉色、pH、嫩度和系水力8项指标测定。

Infectious bovine rhinotracheitis, bovine herpesvirus-1(BHV-1) infection of cattle, has a wide variety of clinical manifestations, known mainly as a kind of respiratory tract disease characterized by rhinotracheitis, conjunctivitis, fever, abortion, infectious pustular vulvovaginitis.

牛传染性鼻气管炎(infectious bovine rhinotracheitis,IBR)是由Ⅰ型牛疱疹病毒(bovine berpesvirus-1,BHV-1)引起牛的一种以上呼吸道炎症为主的接触性传染病,临床表现多种多样,以鼻气管炎、眼结膜炎、高热、流产、传染性脓疱为主要特征。

In this way until the五更suddenly heard inside the mountain,"哗啦啦"sound, mountains, along the northern slope of dazzling golden light off, light-emitting sisters rushed towards the place back and forth, saw only the hole Well, rough fingers, holes along the inside of view, to see there is a gold table, side table, the rows of neatly steamed them Jinniu size.

就这样等到了五更,忽然听见山里面&哗啦啦&作响,山北坡放出一道耀眼的金光,姐妹俩急忙朝发光的地方跑去,只见那洞眼只有手指头那么粗,顺着洞眼往里看,见到内有一张金方桌,方桌上整齐地摆着一排排馒头大小的金牛。

Bank robbery bovine Captain Bush Telegraph Cash Cash Cash Crazy collision Cash Cash with flash riches Cherry Red Emirates Emirates Gold City Magic dollars Cool Cat Tomato seat universe Jack Crazy Crazy E-Fish ...

抢银行 牛眼布什电报现金船长现金碰撞现金疯狂带现金现金闪光樱桃红酋长财宝酋长魔法黄金城酷美圆宇宙猫番茄座椅疯狂杰克疯狂鄂鱼。。。

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