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Clau (5, 10, 20〓) was bound to rat plasma a greater extent than clau, while in rabbit plasma the reverse was the case. By Scatchard plots using bovine serum albumin, clau had a higher equilibrium constant and the greater numbers of binding sites than its antipode.

在与大鼠血浆蛋白的结合中,clau的结合率平均为28.37±0.31(n=3,三个剂量5,10,20μM),clau的结合率平均为38.05±2.61(n=3),clau结合率低于clau;而在与兔血浆蛋白的结合中,clau的结合率平均为47.21±4.91(n=3,三个剂量),clau的结合率平均为41.43±5.21(n=3),clau的结合率略高于clau;在与牛血清白蛋白的结合中,clau的结合率也高于clau,立体选择方向与兔血浆蛋白结合相同,而与大鼠血浆蛋白结合相反,提示存在种属差异;在与系列浓度的牛血清白蛋白结合中,通过Scatchard作图法求得蛋白结合点数和平衡常数;在clau,N和K分别为90.02±35.76和5.326±1.196(n=4);在clau,N和K分别为59.92±20.39和4.203±1.250(n=4),两者的比值分别为1.50和1.27;进一步说明clau对映体的蛋白结合存在立体选择性差异。

As to the formation and evolution of the motives in classical fictions, our viewpoints are: the FuXi custom had engendered an extremely rich repertory of motives, such as JianDi pregnancy legend in the Shang Dynasty, which was the earliest form of the "Swan Maiden" type stories in the world. This type of the story later in the Jin Dynasty evolved into the Maiden-bird type story, which again involved into many other stories in which maidens took the shape of an egret, a swallow, a snail, a tigress, a fairy maiden or a girl in lotus leaves. The custom of HanShi or QingMing may be embodied in the stories about encountering with a girl reviving from a tomb. The dragon in classical fictions may be disguised into an ox or trees, whose fight on fifth of May was a counterevidence of DuanWu.

至于古小说母题的生成和演化方面,我们也许可以认识到:以上巳为主的祓禊风俗产生了最为丰富的小说故事母题,如殷商时期的简翟生子传说是世界上最早的"天鹅处女"型故事,它在晋代又演化为女鸟故事;而那些鹤女、鹄女、鹭女、燕女、螺女、虎女、荷衣女等故事,则在不同程度上是"天鹅处女"型故事的变型型式;在同异境女子遇合的故事中,郑交甫神遇江妃二女、世间男子巧遇仙境女子的故事都包藏着上巳习俗的内涵,而幽婚故事更多地带有寒食、清明风俗的痕迹;古代盛传的金牛故事、青牛故事不但可以归属龙类故事,而且在很大程度上与端午厌胜蛟龙的巫术有关,至于龙斗故事则明显地和端午习俗相联系,甚至可以由此反证端午驱龙的本意。

An antibody binding to IGF-IR and inhibiting the binding of IGF-I and IGF-II to IGF-IR which is characterized in that said antibody is a is ofIgG1 isotype, b shows a ratio of IC50 values of inhibition of the binding of IGF-I to IGF-IR to the inhibition of binding of IG-II to IGF-IR of 1:3 to 3:1, c inhibits for at least 80% at a concentration of 5 nM IGF-IR phospohrylation in a cellular phosphorylation assay using 3T3 cells providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0.5% heat inactivated fetal calf serum when compared to such an assay without said antibody, and d shows no IGF-IR stimulating activity measured as IGF-IR phophorylation at a concentration of 10 M in a cellular phosphorylation assay using 3T3 providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0,5% heat inactivated fetal calf serum when compared to such an assay without said antibody has improved properties in antitumor therapy.

一种已经提高了抗肿瘤治疗的特性的抗体,所述抗体结合IGF-IR并且抑制IGF-I和IGF-II与IGF-IR结合,其特征在于所述抗体a是IgG1同种型,b显示其对IGF-I与IGF-IR结合的抑制的IC 50 值与其对IGF-II与IGF-IR结合的抑制的IC 50 值的比率为1∶3-3∶1,c当与没有所述抗体的这种测定比较时,其在5nM的浓度上,在包含0.5%的热灭活胎牛血清的培养基中,使用3T3细胞的细胞磷酸化测定中,抑制至少80%的IGF-IR磷酸化,所述3T3细胞提供400,000-600,000分子IGF-IR/细胞,和d当与没有所述抗体的这种测定相比,在包含0.5%的热灭活胎牛血清的培养基中,使用3T3的细胞磷酸化测定中,其在10μM的浓度上没有显示作为IGF-IR磷酸化所测量的IGF-IR刺激活性,所述3T3提供400,000-600,000分子IGF-IR/细胞。

The next year I did better still, for I spaded up all the land which I required, about a third of an acre, and I learned from the experience of both years, not being in the least awed by many celebrated works on husbandry, Arthur Young (10) among the rest, that if one would live simply and eat only the crop which he raised, and raise no more than he ate, and not exchange it for an insufficient quantity of more luxurious and expensive things, he would need to cultivate only a few rods of ground, and that it would be cheaper to spade up that than to use oxen to plow it, and to select a fresh spot from time to time than to manure the old, and he could do all his necessary farm work as it were with his left hand at odd hours in the summer; and thus he would not be tied to an ox, or horse, or cow, or pig, as at present.

第二年,我就干得更好了,因为我把总需要量的全部土地统统种上了,只不过一英亩的三分之一,从这两年的经验中,我发现了我没有给那些农业巨著吓倒,包括亚瑟·扬的著作在内。我发现一个人如果要简单地生活,只吃他自己收获的粮食,而且并不耕种得超过他的需要,也不无餍足地交换更奢侈、更昂贵的物品,那末他只要耕几平方杆的地就够了:用铲子比用牛耕又便宜得多;每次可更换一块新地,以免给旧地不断地施肥,而一切农场上的必要劳动,只要他夏天有空闲的时候略略做一做就够了;这样他就不会像日前的人们那样去和一头牛,或马,或母牛,或猪猡,捆绑在一起。

TITLESSOLO CONTEST- Winner of European 1vs1 Ultimate B-Boy session 2 - Germany 1998- Winner of the best B-Boy at BOTY Scandinavia Sweden 2001- Winner of the 1vs1 UK-CHAMPIONSHIP - London 2001- Winner of the TV -show STAR SEARCH - Denmark 2004- 4th place - REDBULL BC-ONE - Switzerland 2004 CREW CONTEST- Winner of the Scandinavian BOTY 2001.2004.2005- Winner of the Scandinavian CIRCLE REVOLVING 2004- Winner of the URBAN GAMES London 2006- Winner of the HIPHOP CONECTION Italy 2006- Winner of the BLOCK PARTY Monpellier France 2006- Winner of the BREAKDANCE SESSION Poland 2004- Winner of the FLOOR WARS Copenhagen Denmark 2006- Winner of BOTY Scandinavia 2005 JUDGE- BATTLE MASTER Korea 2004- B-BOY HODOWN Texas 2005- THE MOST WANTED Catania Italy 2005- CARA A CARA Valencia Spain 2005- BOTY Poland 2005- BOTY East Germany 2006- BOTY France 2006- BOTY Korea 2006- GAMES ON DA FLOOR Igualada Spain 2006- REDBULL BEAT BATTLE London 2006- UK B-BOY CHAMPIONSHIPS 2006- RED BULL BC ONE, Brazil, 2006

业权独奏有奖竞赛-冠军欧洲1vs1极限的B -男孩会议2 -德国1998年-瑞典斯堪的纳维亚得主博特2001年最佳的B -男孩-得主1vs1英国锦标赛- 2001年伦敦-冠军的电视节目新星-丹麦2004-第四名-红牛公元前的ONE -瑞士2004船员有奖竞赛-冠军的斯堪的纳维亚博特2001.2004.2005-冠军2004年的斯堪的纳维亚回转圆-得主城运会2006年伦敦-得主HIPHOP的连接如意大利2006年-得主法国2006块党校蒙佩利尔-会议的霹雳舞冠军2004年波兰-冠军地板的倍率丹麦哥本哈根2006年- 2005年北欧得主博特索非亚- 2004年韩国大师会战-乙男孩HODOWN 2005年得克萨斯州-你最期待的2005年意大利卡塔尼亚-拉一拉2005年西班牙瓦伦西亚-博特2005年波兰-博特2006年东德- 2006年法国博特- 2006年韩国博特-游戏伤残地板西班牙伊瓜拉达2006-红牛拍会战2006年伦敦-英国乙男孩锦标赛2006-红色公牛年代的ONE,巴西,2006

TITLES SOLO CONTEST - Winner of European 1vs1 Ultimate B-Boy session 2 - Germany 1998 - Winner of the best B-Boy at BOTY Scandinavia Sweden 2001 - Winner of the 1vs1 UK-CHAMPIONSHIP - London 2001 - Winner of the TV -show STAR SEARCH - Denmark 2004 - 4th place - REDBULL BC-ONE - Switzerland 2004 CREW CONTEST - Winner of the Scandinavian BOTY 2001.2004.2005 - Winner of the Scandinavian CIRCLE REVOLVING 2004 - Winner of the URBAN GAMES London 2006 - Winner of the HIPHOP CONECTION Italy 2006 - Winner of the BLOCK PARTY Monpellier France 2006 - Winner of the BREAKDANCE SESSION Poland 2004 - Winner of the FLOOR WARS Copenhagen Denmark 2006 - Winner of BOTY Scandinavia 2005 JUDGE - BATTLE MASTER Korea 2004 - B-BOY HODOWN Texas 2005 - THE MOST WANTED Catania Italy 2005 - CARA A CARA Valencia Spain 2005 - BOTY Poland 2005 - BOTY East Germany 2006 - BOTY France 2006 - BOTY Korea 2006 - GAMES ON DA FLOOR Igualada Spain 2006 - REDBULL BEAT BATTLE London 2006 - UK B-BOY CHAMPIONSHIPS 2006 - RED BULL BC ONE, Brazil, 2006

业权 独奏有奖竞赛-冠军欧洲1vs1极限的B -男孩会议2 -德国1998年-瑞典斯堪的纳维亚得主博特2001年最佳的B -男孩-得主1vs1英国锦标赛- 2001年伦敦-冠军的电视节目新星-丹麦2004 -第四名-红牛公元前的ONE -瑞士2004 船员有奖竞赛-冠军的斯堪的纳维亚博特2001.2004.2005 -冠军2004年的斯堪的纳维亚回转圆-得主城运会2006年伦敦-得主HIPHOP的连接如意大利2006年-得主法国2006块党校蒙佩利尔-会议的霹雳舞冠军2004年波兰-冠军地板的倍率丹麦哥本哈根2006年- 2005年北欧得主博特索非亚- 2004年韩国大师会战-乙男孩HODOWN 2005年得克萨斯州-你最期待的2005年意大利卡塔尼亚-拉一拉2005年西班牙瓦伦西亚-博特2005年波兰-博特2006年东德- 2006年法国博特- 2006年韩国博特-游戏伤残地板西班牙伊瓜拉达2006 -红牛拍会战2006年伦敦-英国乙男孩锦标赛2006 -红色公牛年代的ONE,巴西,2006

In addition, research reveals that in many cases, eating "free-range" and "organic" meat, dairy, and eggs does not substantially reduce greenhouse gas emissions, because free-range cattle, for example, are not fattened as quickly as feedlot cattle, so the reductions in carbon dioxide equivalents in foregoing chemical fertilizers and pesticides are nearly offset by these factors.

此外,研究也揭示:在许多案例中,吃&自由放养&的和&有机&的肉、乳制品和蛋实际上并未减少温室气体的排放。例如,自由放养的牛长胖的速度不如养殖场的牛,所减少的二氧化碳排放量大体跟前面提到的化肥和农药等因素造成的碳排量等值,正好两相抵消。

Sections were washed in TBS,mounted with Vectashield mounting medium (VectorLaboratories Inc., Burlingame, CA, USA), and analyzedon a laser-scanning confocal microscope.

切片被放置在含有LC-3抗原(1:1,000)的TBS中4度冰箱过夜后,再用0.1%的Triton X-100和含有0.1%的牛血清蛋白的TBS清洗,用荧光标识的抗兔的二抗在含有1%的牛血清蛋白的TBS缓冲液中放置1小时,封片后供激光共焦显微镜分析结果。

The proliferative capacity of LEC from human had directly relevance to the age of donor(r=-0.996).Conclusions The formation of lens-like spherules is characteristics of LEC lines in the cultured cells.Under identical conditions,the proliferative rate of LEC from bovine and rabbit is fast than that of LEC from human,but dedifferentiation of LEC from bovine and rabbit is easier than that of LEC from human;LEC from the three species exhibit similar limited growth potential.The proliferative rate of LEC from human has a inversely proportion with age.

&晶状体小体&的形成可作为确定晶状体上皮细胞株的一项特征性依据,而体外培养的人、牛、兔晶状体上皮细胞具有相同的有限生长潜能,在相同的条件下,牛、兔晶状体上皮细胞的生长增殖速度比人晶状体上皮细胞快,但易于发生去分化;此外,人晶状体上皮细胞的生长增殖率与年龄密切相关,年龄越小,晶状体上皮细胞的生长增殖速度越快。

The result would be used in tobacco breeding.4 SRAP technology is used to study the genetic differences among different types of tobacco, including flue-cured tobacco, oriental tobacco, maryland tobacco, burley tobacco, and air/sun cured tobacco, five pairs of primer combinations which can amplify characteristic bands has been screened. Among the five combinations, two of them (SF25*SR2 and SF3*SR26) can distinguish air-cured tobacco including maryland tobacco and Burley tobacco from the other cultivated forms; SF20*SR14 can distinguish sun cured tobacco including oriental tobacco and sun-cured tobacco; SF16*SR14 can distinguish flue-cured tobacco and air-cured tobacco, and SF22*SR14 can distinguish DBJ599 with specisl aroma and the other flue-cured tobacco.5 8 varieties , which represent the different types of tobacco, are divided into 3 groups. I: G-28, II:DBJ599、MD40、By21, III: Basma、Sumsun、XNS、QCT. The cluster analysis result es consistant with experience evatuation.6 Anorthogonal design was used to optimize a ILP-PCR system with 5 factors (DNA, Mg2+, dNTP, primer and Taq polymerase) at 4 levels.The result showed the optimized ILP-PCR system for Nicotiana tabacum L. was: 2uL10×PCRbuffer, 20ng template DNA, Mg2+ 2.0mmol/L, dNTP 150umol/L, primer 0.3umol/L, Taq DNA polymerase 1 U in a total of 20uL reactionsolution.

研究结果可作为烤烟与香料烟类型间的杂种鉴定利用的实验与理论依据。4、利用SRAP标记技术,研究和分析了烤烟、晒烟、晾烟、白肋烟、香料烟等不同栽培类型间的基因型差异,初步筛选出了能够在分子水平上反映不同烟草类型差异的特征性标记带:晾烟MD40、Ky17、Burley21、TN90基因型共有的特征带有两对引物组合,分别为SF25*SR2和SF3*SR26;晒烟小牛舌、青梗、小花青和香料烟Sumsun、komotini Basma、Xanthi Basma共有的特征带引物组合为SF20*SR14;NC89、K326、G-28、大白筋和MD40、Ky17、Burley21、TN90共有的特征带引物组合为SF16*SR14,烤烟中大白筋599的特征带引物组合SF22*SR14.5、利用61对SRAP引物组合,研究了普通烟草不同类型间的亲缘关系,将普通烟草类型间8个不同供试材料划分为3类别。I:烤烟类型G-28II:白肋烟类型(MD40、By21、大白筋599III:香料烟类型(Basma、Sumsun、小牛舌、千层塔)。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。