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To evaluate the specificity of the PCR, genomic DNA of Theileria annulata,Babesia bovis,Toxoplasma gondii,Leishmania donovani and standard strain of N. caninum were used as a template in the PCR. For determining the detection limit of amplification procedure, PCR was run on a dilution series of genomic DNA from N. caninum(1.562 5-200 ng/ml). Brain tissue samples of 32 aborted fetuses were detected by PCR-based assay, and 23 blood samples from mothers were tested by ELISA. Results The amplified DNA fragment (350 bp)had a high identity of 98% with the Nc-5 gene sequence of N.

以环形泰勒虫、牛巴贝斯虫、刚地弓形虫、杜氏利什曼原虫以及犬新孢子虫标准株DNA为模板进行扩增以验证PCR的特异性,采用紫外分光光度计测定犬新孢子虫标准株DNA浓度和纯度,取高纯度的DNA样品用灭菌水稀释,分别取不同量的DNA进行PCR扩增,确定PCR方法的敏感性;利用该方法对32份奶牛流产的胎牛脑组织样品进行检测,同时,对其中23份流产的母牛血样进行ELISA血清学检测,以评价犬新孢子虫PCR方法的检测效果。

For the enlightenment of those who are not so intimately acquainted with the minutiae of the municipal abattoir as this morbidminded esthete and embryo philosopher who for all his overweening bumptiousness in things scientific can scarcely distinguish an acid from an alkali prides himself on being , it should perhaps be stated that staggering bob in the vile parlance of our lower class licensed victuallers signifies the cookable and eatable flesh of a calf newly dropped from its mother

这位有着病态精神的审美学兼胚胎哲学家,尽管连酸与碱都分不清,在科学知识上却摆出一副傲慢自负的架子。为了启发那些对市立屠宰场的细节没他那么熟悉的人们,也许应该在此说明一下:我们那些拥有卖酒执照的低级饮食店的俚语小牛崽肉,指的就是打着趔趄的牛崽子280那可供烹调食用的肉。

For the enlightenment of those who are not so intimately acquainted with the minutiae of the municipal abattoir as this morbidminded esthete and embryo philosopher who for all his overweening bumptiousness in things scientific can scarcely distinguish an acid from an alkali prides himself on being, it should perhaps be stated that staggering bob in the vile parlance of our lower class licensed victuallers signifies the cookable and eatable flesh of a calf newly dropped from its mother.

为了启发那些对市立屠宰场的细节没他那么熟悉的人们,也许应该在此说明一下:我们那些拥有卖酒执照的低级饮食店的俚语小牛崽肉,指的就是打着趔趄的牛崽子[280]那可供烹调食用的肉。

For the enlightenment of those who are not so intimately acquainted with the minutiae of the municipal abattoir as this morbidminded esthete and embryo philosopher who for all his overweening bumptiousness in things scientific can scarcely distinguish an acid from an alkali prides himself on being, it should perhaps be stated that staggering bob in the vile parlance of our lowerclass licensed victuallers signifies the cookable and eatable flesh of a calf newly dropped from its mother.

为了启发那些对市立屠宰场的细节没他那么熟悉的人们,也许应该在此说明一下:我们那些拥有卖酒执照的低级饮食店的俚语小牛崽肉,指的就是打着趔趄的牛崽子那可供烹调食用的肉。

The three types of reconstructed embryos(reconstructed embryo of goat-rabbit, goat-bovine and goat-goat) were produced respectively by nuclear transfer using goat ear firbroblast cells as donors and rabbit, bovine and goat ooctyes as recipients, and the factors of cytoplast environments influencing cloned embryo development in vitro, cytoplast effect in nuclear transfer, the methods of transferring cloned embryo and the effects of pregnancy factor on implantation of cloned embryo were studied in this experiment, in order to supply basis for solving the difficulty of implantation of inter-species cloned embryo in recipient and improving the efficiency of cloning.

本试验以波尔山羊耳成纤维细胞为供体,分别以牛、羊、兔的卵母细胞为受体进行体细胞核移植,构建了山羊-兔、山羊-牛和山羊-山羊三种重构胚。研究了不同的胞质环境对重构胚体外发育的影响与核移植中的胞质效应,并对异种克隆胚胎采用了去透明带移植和配种后再移植的方法,探讨移植方法和妊娠因子对异种克隆胚胎在受体动物体内发育的影响,旨在为解决异种克隆胚移植不易着床发育的难题和为提高动物克隆效率提供理论依据。

It is necessary to develop a protein-free media for oocytes maturation that can prevent zona hardening. In this study, immature macaque oocytes were cultured in modified CMRL media alone or CMRL with either bovine calf serum or PVA for 24 hours. Also, in vivo matured oocytes were cultured in CMRL supplemented with PVA or serum for approximately 6 hours.

我们在本研究中,将非成熟的猕猴卵母细胞分别在改良的CMRL,或在含有胎牛血清或聚乙烯醇的改良CMRL培养基中培养24小时;同时,将体内成熟的卵母细胞分别在含有PVA或者胎牛血清的CMRL中培养6小时,然后进行体外授精,比较各组间精子穿透率或受精,以及胚胎发育情况。

The approaching Chinese New Year of 2009 is a good occasion to get together and thus Ox Warehouse is inviting all the 2003 Ox Exhibition participating artists, as well as new creative people, to fill in different colors at this special occasion and join this re-exhibition, looking at creation in a more free and relaxing way.

踏入2009年的新春,我们共聚一堂,牛房希望再次邀约当年曾参展的艺术家及一些创作新人类,为新春开年注入不同的色彩,也让创作这回事变得自在、轻松起来,对进入第六个年头的牛房仓库,回望也好,期望也好,希冀透过不同的展示方式保持独特的艺术气象。

Because solid fat has polymorphism and special composition, it can bind liquid fat. Beef tallow was added into oil phase to improve the stability of artificial beef fat. Third-grade soybean oil took the place of plant oil iii proportion to improve the color of beef fat.

在前期研究的基础上,通过在油相中添加牛油,应用油脂的同质多晶型特点及牛油的特殊组成来提高肥牛脂肪的稳定性;用一级大豆油与三级大豆油适当调配,来改善肥牛脂肪的颜色。

In particular, one unique is the selection of - sirloin steak from cattle outside the ridge in part, primarily by cattle on the waist of the fillet composition of the meat of the extension of white meat with a lap bar, and contains some fat oil, oily and delicious meat so; unique 2 in its texture - more like filet mignon with lean meat compared to the West of cold steak taste seems more toughness, meat is also more rigid, more chewy, suitable for people who enjoy good teeth.

具体而言,其独特之一在于选材——西冷牛排取自位于牛外脊部分,主要由牛上腰部的脊肉构成,在肉的外延带一圈呈白色的肉筋,并且含有一定肥油,因而肉质油润而鲜美;独特之二在于其质感——与瘦肉较多的菲力牛排比起来,西冷牛排的口感显得更有韧度,肉质也偏硬,更有嚼劲,适于牙齿较好的人享用。

Sodium propionate and sodium pyruvate could promote liver gluconeogenesis by up-regulating the abundance of PEPCK-C mRNA in the hepatocytes, but the up-regulation was limited.

表明,丙酸钠、丙酮酸钠能通过上调体外培养的新生犊牛肝细胞PEPCK-C mRNA的表达而促进肝糖异生代谢,但上调作用是有限的;胰岛素能通过下调体外培养的新生犊牛肝细胞PEPCK-C mRNA的表达而抑制肝糖异生代谢,且下调作用呈剂量依赖性;胰高血糖素与胰岛素作用刚好相反;瘦蛋白未起直接的调节作用。

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推荐网络例句

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。