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The fragment size can be amplified by 295 bp through the primer, in order to prevent false positive from appearing, a pair of internal control primers C34 is designed through the invention according to a bull autosome 3 reported sequence, the fragment size is amplified by 208bp, dual PCR amplification is performed to single bull sperm through the two pairs of primers, and then the final evaluation is performed to the sperm separation purity according to the statistical analysis to the detection result.

本发明提供了一种检测X、Y精子分离纯度的引物，该引物是针对牛Y染色体上性别决定基因Sry通过PCR错配技术设计而成，通过该引物可扩增片段大小为295bp，为了防止假阳性出现，本发明根据牛3号常染色体报道序列设计了一对内标引物C34，扩增片段大小为208bp，通过上述两对引物对单个牛精子进行双重PCR扩增，然后根据对检测结果的统计分析，对分离精子纯度做出最终评价。

The results showed that typical FMD clinical signs produced in cattle inoculated intradermally with pathogenic FMDV after 48 h. The 3ABC antibody produced in these cattle were detected to be suspicious on the 3rd day, 3ABC antibody in the 1/4 cattle were positive on the 5th day, and the 4/4 were positive on the 8th day post virus inoculation; In 10 co-housed cattle (breeded with artificially infected pigs and sheep separately), no 3ABC antibody were detected on the 7th day and percent of positive 3ABC antibody was only 30%(3/10) on the 15th dpi (4/10 had clinical symptom).

实验表明，4头牛在舌面接种感染FMDV 48h后全数发病，第3天即出现可疑的3ABC抗体，第5天检测到阳性的3ABC抗体，至第8天3ABC抗体全部为阳性；10头同居牛（分别与接种感染猪和羊同居）在同居第7天时未检出3ABC抗体，在同居第15天时，3ABC抗体阳性率仅为30%；肌肉接种病毒羊3ABC抗体产生时间与接种病毒牛一致。

Thirty crossbred beef steers (Limousin + local yellow ), aged eight months to twelve months, were fed in the crib and randomized complete block design and differentiated weight by block. The cattle were divided into six nutrition pattern groups.

摘 要：本试验选用8～12月龄的利木赞杂交牛30头，栓系舍饲，采用随机完全区组设计将试验牛分成6个营养模式组，进行300天的育肥后，对所有试验牛进行屠宰，测定其胴体质量和肉品品质。

The total RNA was abstracted from the larvae of Boophilus microplus, and a 1982bp Bm86 gene was amplified by RT-PCR. The target gene was subcloned into T vector. The sequencing showed that the nucleotide sequence of the cloned Bm86 gene shared 97.4% homology with the data published in and this fragment contained the complete open reading frame of Bm86 gene.

为了克隆微小牛蜱Bm86 基因及构建该基因的表达载体，以微小牛蜱饥饿幼蜱的破解物提取的总RNA为模板，参照已发表的微小牛蜱Bm86基因的核苷酸序列，设计了1对引物，采用RT-PCR技术获得微小牛蜱Bm86基因；将Bm86基因克隆入载体，并进行序列分析，结果证明，克隆的Bm86基因序列与GenBank上登录的Bm86基因序列的同源性达97.4%，而且该序列包含完整的开放阅读框。

Their relative molecular weights were 4785 and 4386 respectively. And their absorbance peaks were 210.5 nm and 225.4 nm respectively. The bovine and caprine placental peptides could improve immunosuppressive T lymphocyte transformation rate. The immunosuppressive lymphocyte transformation rates in groups with the bovine placental peptide of 1:1×10^3 and 1:1×10^4 (P.05)and with the caprine placental peptides of 1:1×10^2, 1:1×10^3 and 1:1×10^4 (P.01 )increased more remarkably than that in immunosuppression group.

结果 从牛和羊胎盘中成功提取出了胎盘肽，其相对分子质量分别为4785和4386；其最大吸收峰分别为210.5和225.4nm；牛和羊胎盘肽均可使顺铂抑制的兔外周血T淋巴细胞的转化率显著提高，其中牛胎盘肽以1：l×10^3、1：l×10^4 2组吸收度明显高于抑制组(P.05)；羊胎盘肽以1：1×10^2、1：1×10^3和1：1×10^3 3组效果最为明显(P.01)。

MethodsThe distilled bovine and caprine placental peptide s were prepared by ultrafiltering and their relative molecular weights were mensurated by improved Tricine-SDS-PAGE eletrophoresis with urea.

结果从牛和羊胎盘中成功提取出了胎盘肽，其相对分子质量分别为4 785和4 386；其最大吸收峰分别为210.5和225.4 nm；牛和羊胎盘肽均可使顺铂抑制的兔外周血T淋巴细胞的转化率显著提高，其中牛胎盘肽以1∶1×103、1∶1×1042组吸收度明显高于抑制组(P.05)；羊胎盘肽以1∶1×102、1∶1×103和1∶1×1043组效果最为明显(P.01)。

The study on the extracting small experiment of Choleic acid, Glycocholic acid and Taurocholic acid.The extracting small experiments of Choleic acid, Glycocholic acid and taurocholic acid were studied by adopting deproteinizatio, depigmentation, extraction and column chromatographic fractionation.

提取胆酸、甘氨胆酸、牛磺胆酸的小试工艺研究采用脱蛋白、除色素、萃取、柱层析分离等方法开展了从牛羊混合胆汁中分离、提取和纯化胆酸、甘氨胆酸及牛磺胆酸三种有效成分的小试提取工艺研究。

After Tess had settled down to her cow there was for a time no talk in the barton, and not a sound interfered with the purr of the milk-jets into the numerous palls, except a momentary exclamation to one or other of the beasts requesting her to turn round or stand still.

把所有的牛都算起来，克里克管理的奶牛有一百头；在这一百头牛里，有六头或八头牛是奶牛场老板自己动手挤奶，除非是他出门离开了家。

Each mother must quickly bond with her calf, for if the herd stampedes, the mother and her calf could easily become separated, and the calf would have little chance of survival on its own.

母牛羚必须很快与自己的孩子建立起联系纽带，因为如果牛羚群受惊狂奔，母牛羚和小牛羚会很容易走散，而小牛羚靠它自己生存下去的机会几近于零。

Tonight, by about 8:00 p.m., the waning gibbous moon and the constellation Taurus the Bull will appear together over your eastern horizon. Thereafter, the moon will shine in front of the Bull for the rest of the night.The radiant points for two November meteor showers – the South Taurids and North Taurids – both reside in front o...

我想不是说两个一模一样的星座，流星雨的名称是根据其辐射点所出现的星座范围而定的，出现在金牛座南边的就叫南金牛座流星雨，出现在金牛座北边的就叫北金牛座流星雨，出现在猎户座范围内的就叫猎户座流星雨。。。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。