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Additionally,this study developed three species-spcific real-time fluorescence quantitative PCR-based assays for rapidly, specificity, sensitivity,detecting Brucella abortus, Brucella melitensis, Brucella suis.

在建立布鲁氏菌属荧光定量PCR检测方法的基础上,根据布鲁氏菌基因组多拷贝插入元件IS711及其下游多态位点,设计了检测牛、羊、猪种布鲁氏菌的引物和牛、羊、猪种特异性荧光探针,优化了牛、羊、猪种布鲁氏菌荧光定量PCR反应体系和条件,建立了牛、羊、猪种布鲁氏菌荧光定量PCR检测方法,组装了牛、羊、猪种布鲁氏菌检测试剂盒,该方法能特异检测上述3个种的布鲁氏菌。

Huangpu Town, Zhongshan City Niu Li maschinenbau 13823156967 Luosheng are handling the production of hydraulic machinery specialized factories, is an advanced manufacturing technology by relying on foreign countries and the rise of emerging enterprises, with a wealth of experience and solid strength, in order to provide security clients and high-efficiency, multi-angle logistics handling equipment; Huangpu Town, Zhongshan City Niu Li maschinenbau main products are: hand pallet trucks, manual hydraulic elevated cars, increased vehicle electro-hydraulic, self - increased hydraulic vehicles, hydraulic lift platform, hydraulic lift car tail plate, tank trucks, platform trucks carrying more than ten kinds of hydraulic equipment, we can according to customer demand for the development of new products; Zhongshan huangpu Cow town Machinery factory is the only set auto hydraulic lift tail plate development and the development, production and sales of integrated production enterprises, the product has passed the production of AQSIQ license certification, 70% of export to Hong Kong Products , Macao, Taiwan and other regions,"cow power" brand auto hydraulic lift tail plate has become a well-known domestic and foreign large enterprises and government agencies preferred brand; cow power Zhongshan maschinenbau domestic product are hand pallet trucks professional one factory, with an annual output has reached 50,000 units, the production of "cow power" brand hand pallet trucks have been the German TUV Certification Body of GS / CE certificate.

中山市黄圃镇牛力机械制造厂罗生13823156967是生产液压搬运机械的专业厂,是一个依托国外先进制造技术而掘起的新兴企业,拥有丰富的经验和雄厚的实力,为客户提供安全、高效率的、多角度的物流搬运设备;中山市黄圃镇牛力机械制造厂主要的产品有:手动液压搬运车、手动液压升高车、电动液压升高车、自行式液压升高车、液压升降平台、汽车液压升降尾板、油桶车、平台车等十余种液压搬运设备,我们可根据客户的需求开发各种新产品;中山市黄圃镇牛力机械制造厂是国内唯一集汽车液压升降尾板的开发与研制,批量生产以及销售的一体化生产企业,产品已通过国家质检总局的生产许可认证,产品70%出口港、澳、台等地区,"牛力"牌汽车液压升降尾板已经成为国内外众多知名企业及政府机关的首选品牌;中山市牛力机械制造厂是国内生产手动液压搬运车的专业工厂之一,年产量已达到50000台,生产的"牛力"牌手动液压搬运车已获得德国TUV认证机构的GS/CE证书。

Cuisine: Food here owns has all characteric of rural family. Stewed beef and taro is made of the sirloin of old ox's. The boss said the sirloin certainly must use old ox's sirloin which tills the fields, because the meat has more flavor than the usual cattle's. Stew sirloin and taro together with Chinese cinnamon and spice. These material has perfect combination of the taste, and the sirloin has transparent look, and the taro is cooked soft and loose. Combining some taro to rice forms the nice flavour of rice.

菜品特写:在农舍吃饭很有农家菜的口味特点,比较有代表性的就是这道芋头炖牛腩,老板说牛腩一定要用那种耕田的老黄牛的牛腩,饲养的牛味道就会差很多,把牛腩和芋头一起清炖,再放些桂皮、花椒等香料让菜充分地进味,牛腩通体透明,肉烂而不松,芋头炖得粉粉的,有一部分甚至成了芋头泥,和在饭里很是好吃。

Furthermore, the genetic distances in the HSL genes are much smaller between yak, cattle, and zebu than those between each of the three species and the buffalo. Therefore, it is reasonable to consider yak as an independent species of the genus Bos.

牦牛、普通牛和瘤牛3个物种间的遗传距离远小于它们各自与水牛这一物种的遗传距离,它们三者之间的亲缘关系也相对于它们各自与水牛间的亲缘关系都较近,故将牦牛、普通牛和瘤牛划分在同一个属——牛属更为合理。

The result shows that firstly,zebu ismost closely related to cattle;secondly,closely related to yak,buffalo,pig,human,lastly,related to rat and mouse.The results of the phylogenetic trees reflect the molecularevolution relationship among these species,consistent with the zoological classification.The genetic distances among yak,cattle and zebu were relatively small,but geneticdistances between these three species and buffalo were relatively large.

研究表明,牦牛、普通牛和瘤牛三个物种间的遗传距离远小于它们各自与水牛这一物种的遗传距离,三者之间的亲缘关系也相对于它们与水牛间的亲缘关系较近,故将牦牛、普通牛和瘤牛划分在同一个属—牛属,而将水牛划分在另一个属更为合理。

Particularly,the CP content of H.compressa from Caoba is 3.0% higher than that of H.compressa cv.Chonggao.Therefore,there is a promising for H.compressa from Caoba to offer an alternative to be a new variety.By the clustering analysis,all native accessions are classified into four different types,which is helpful to choose and utilize further.

经测定,大邑生态型扁穗牛鞭草和草坝生态型扁穗牛鞭草表现出植株高大、茎粗壮、叶量丰富、干物质含量高等许多牧用性饲草的优良特点,特别是草坝生态型扁穗牛鞭草的粗蛋白质含量比重高牛鞭草高约3.0%,所以,它有望成为优良的扁穗牛鞭草新品种。

Immediately after he was born,hermes was appointed godof thieves at olympus.and a thief he became when he was barely a few hours old.feeling hungry,the infant left his cradle after nightfall to hunt for food.he chanced uponapollo''s oxen grazing on a meadow and drove a number ofthese animals away,tying tree branches around the feet of thecattle to make their footsteps dim.he killed two of these oxenand had a magnificent dinner.then he slipped back into his littlebed in his mother''s house.when apollo came to question himhermes pretended to be innocent.the angry sungod draggedhim up to olympus,where he was accused of the stealing andmade to return the cattle to their master.in reconciliation little hermes gave apollo the lyre he had made out of a tortoise shell,and apollo was so pleased with the gift that he presented his little brother with a magic stick.the stick could pacify hostileforces.

海尔墨斯一生下来就被任命为奥林波斯山的贼神。他出生后几个小时的时候就做了贼。黄昏时分,他感到饥饿,于是还是婴孩的海尔墨斯离开了他的摇篮去找食物吃。他碰巧看见阿波罗的牛在草地里吃草,于是他偷走了许多牛,并将树枝绑在牛脚上,让脚印变得模糊不清。他杀了其中两头牛,美餐了一顿。然后悄然地回到他妈妈的房里,爬上床。当阿波罗发现牛少了,来质问他时,海尔墨斯佯作不知。愤怒不已的太阳神把他拖到奥林波斯山上,告他偷窃并责令将牛如数还给主人。为了平息这件事,小海尔墨斯将他用乌龟壳做的琴送给阿波罗。阿波罗得到这件礼物非常高兴,并送给他的小兄弟一支魔棍,这支魔棍可以化解敌对力量。

Immediately after he was born,hermes was appointed godof thieves at olympus.and a thief he became when he was barely a few hours old.feeling hungry,the infant left his cradle after nightfall to hunt for food.he chanced uponapollo\'\'s oxen grazing on a meadow and drove a number ofthese animals away,tying tree branches around the feet of thecattle to make their footsteps dim.he killed two of these oxenand had a magnificent dinner.then he slipped back into his littlebed in his mother\'\'s house.when apollo came to question himhermes pretended to be innocent.the angry sungod draggedhim up to olympus,where he was accused of the stealing andmade to return the cattle to their master.in reconciliation little hermes gave apollo the lyre he had made out of a tortoise shell,and apollo was so pleased with the gift that he presented his little brother with a magic stick.the stick could pacify hostileforces.

海尔墨斯一生下来就被任命为奥林波斯山的贼神。他出生后几个小时的时候就做了贼。黄昏时分,他感到饥饿,于是还是婴孩的海尔墨斯离开了他的摇篮去找食物吃。他碰巧看见阿波罗的牛在草地里吃草,于是他偷走了许多牛,并将树枝绑在牛脚上,让脚印变得模糊不清。他杀了其中两头牛,美餐了一顿。然后悄然地回到他妈妈的房里,爬上床。当阿波罗发现牛少了,来质问他时,海尔墨斯佯作不知。愤怒不已的太阳神把他拖到奥林波斯山上,告他偷窃并责令将牛如数还给主人。为了平息这件事,小海尔墨斯将他用乌龟壳做的琴送给阿波罗。阿波罗得到这件礼物非常兴奋,并送给他的小兄弟一支魔棍,这支魔棍可以化解敌对力量。

Hermes immediately after he was born,hermes was appointed godof thieves at olympus.and a thief he became when he was barely a few hours old.feeling hungry,the infant left his cradle after nightfall to hunt for food.he chanced uponapollo''s oxen grazing on a meadow and drove a number ofthese animals away,tying tree branches around the feet of thecattle to make their footsteps dim.he killed two of these oxenand had a magnificent dinner.then he slipped back into his littlebed in his mother''s house.when apollo came to question himhermes pretended to be innocent.the angry sungod draggedhim up to olympus,where he was accused of the stealing andmade to return the cattle to their master.in reconciliation little hermes gave apollo the lyre he had made out of a tortoise shell,and apollo was so pleased with the gift that he presented his little brother with a magic stick.the stick could pacify hostileforces.

海尔墨斯海尔墨斯一生下来就被任命为奥林波斯山的贼神。他出生后几个小时的时候就做了贼。黄昏时分,他感到饥饿,于是还是婴孩的海尔墨斯离开了他的摇篮去找食物吃。他碰巧看见阿波罗的牛在草地里吃草,于是他偷走了许多牛,并将树枝绑在牛脚上,让脚印变得模糊不清。他杀了其中两头牛,美餐了一顿。然后悄然地回到他妈妈的房里,爬上床。当阿波罗发现牛少了,来质问他时,海尔墨斯佯作不知。愤怒不已的太阳神把他拖到奥林波斯山上,告他偷窃并责令将牛如数还给主人。为了平息这件事,小海尔墨斯将他用乌龟壳做的琴送给阿波罗。阿波罗得到这件礼物非常高兴,并送给他的小兄弟一支魔棍,这支魔棍可以化解敌对力量。

Together with the cited sequences of other species (cattle, zebu cattle and buffalo), the molecular phylogenetic tree was therefore built to probe phylogenetic relationship between Bovine subfamilies, Total 5 variation sites were examined and two types of mutation, transition and transversion, were observed, with the overage percentage of nucleotide variation of 1.66%. It demonstrated the poverty of polymorphism at exon 5 of GH gene of three bovine species. There was only one missense mutation out of 5 mutation sites which led to the shift between leu and val amino.

结果表明,在雷琼牛、巴州牦牛和巴州蒙古牛中共检测到5个变异位点,核苷酸取代方式仅有转换和颠换,核苷酸平均突变率仅为1.66%,说明这3个牛种GH基因外显子5多态性较为贫乏;5个变异位点中仅有1个为错义突变,导致亮氨酸和缬氨酸的转换,但该突变在牛种中分布有差异。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

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