牛

For the purpose of constructing mammary gland specific expressional vector, we use the updream regulation sequence of bovine BLG to regulate lacS gene to express in mammary gland. Positive clone cells are selected and purified by the expression Neo gene and report gene EGFP. So the cells can be used as sources to create transgenic animals by nuclear transfer techniques.

本研究以牛β-乳球蛋白基因上游调控成分作为启动子，嗜热菌糖苷酶基因lacS为目的基因，构建了lacS基因乳腺特异表达载体，并通过脂质体介导法转染体外培养的牛胎儿成纤维细胞，借助新霉素抗性基因和增强型绿色荧光蛋白报告基因来筛选、纯化阳性克隆细胞，为利用核移植技术制备转基因动物提供细胞来源。

A total of 44 cows was selected with more than 12-tons milk yield with a mean of 44 months of age after 12 times of milk test in average, based upon number of milk test equivalent to their second lactation, in 2006. Among of them, 8 out of 44 had more than three lactations.

再分析2006年测乳达12公吨以上乳量的牛只有44头，其平均月龄为44月，平均测乳次数12次；其中有8头是三胎以上的牛。

9 healthy adult Holstein cows were selected as experimental cows and they were divided into 3 groups according to different milk lactations. Individual tracking method was used to observe and record the amount of Diptera insects. The blood was took on the tail vein under the aseptic condition and then the serum was extracted. The level of ACTH, COR, PRL, GH and NE in the serum were detected by the method of radioactive immunoassay method and fluorescence spectrophotometry.

方法］选择9头成年健康的荷斯坦奶牛作为试验牛，按不同泌乳阶段分3组，采用个体跟踪法观察并记录侵袭试验牛双翅目昆虫的数目，自尾静脉无菌采血，提取血清，采用放射性免疫法和荧光分光光度法测定血清中AGTH、COR、PRL、GH和NE的水平。

Me: Yes the West Lake Spaciality Duck Breast Hang pepper beef chive fried yams and a beef ribs is it?

好的，西湖酱鸭、杭椒牛柳、韭黄炒山药和一份牛排骨，是吗？

The next leanest is ground round (for 218 calories, 13 grams of fat), then ground chuck and ground beef (both about 231 calories, 15 grams of fat).

还有一种能够瘦身的牛精肉，它含有218卡路里，13克脂肪，然后是牛颈肉和绞细牛肉，它们都含有231卡路里，15克脂肪。

As an added bonus, witness America's only daily cattle drive as Texas Longhorns are driven right through the heart of the Stockyards.

还有另一好处，是目睹美国唯一每天赶德州的长角牛到&销售与屠宰的牛栏&。

As an added bonus, witness America's only daily cattle drive as Texas Longhorns are driven right through the heart of the Stockyards.

游客还有一个额外的好处，就是观赏美国唯一的每日赶牛活动，看着德州长角牛被驱赶着通过牲畜围栏区中央。

Cattle raising, long an important industry in Texas, became even more flourishing after the war, when enterprising men began to drive their Texas longhorns north across the open public domain.

在得克萨斯州，养牛早就是重要事业，战后尤见繁荣，大胆的赶牛人赶着得州的长角牛，穿过开放的公共地带，向北走去。

Cattle-raising, long an important industry in Texas, became even more flourishing after the war, when began to drive their Texas longhorns north across the open public domain.

在得克萨斯州，养牛早就是重要行业，战后尤见繁荣，大胆的赶牛人赶着德州的长角牛，穿过开放的公共地带，向北走去。

A remarkable example is the low-set Corgi , perhaps one foot tall at the shoulders , that can drive a herd of cows many times its size to pasture by leaping and nipping at their heels .

一个显著的例子是一种低地威尔士矮脚狗，肩高不超过1英尺，却能通过跳跃和咬牛的后脚跟的办法，将一群身高数倍于自己的牛赶到牧场去。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。