牛

When assessing the strategy combinations in the future, the aquaculture biotechnology companies will be allowed to put emphasis on the investment of "Cash Cows", including the high quality fries and the production technology, its will bring the companies lots of profit. But the "Stars", including production equipments, the prevention and control of fish disease, the feed supplements and the environmental improvements, under industry the investment, it then will possibly take the companies toward the direction of the "Cash Cow" enterprise and gain munificent income. When the companies still have funds available, they may consider to investing in "Problem Children" enterprise, including the transgenic, the functional gene, the new variety research and the biology repair medicament. These enterprises still have the development potential, but in contrast they are operated more riskily.

三、未来水产养殖生技公司在进行策略组合评估时，可以考虑将投资重点础b金牛事业，包括优质水产种苗以及生产技术，其未来较能带给厂商业者丰富之利润；而明日之星事业包括生产设备、鱼病防治、饲料添加物以及环境改良等四项，在业者投资下，其可能往金牛事业的方向前进而带给公司丰厚之收益；当公司厂商仍有余裕资金时，可以考虑投资潜力事业包括了基因转殖、弁邬夆穧、新品种研发以及生物修补剂，这些事业仍具发展潜力，但相对也比较具有风险性。

A PET/AL/PP packed retort porch bovine runnet was developed by pretreatment,precooking,frying,soaking,vacuum packaging and sterilization.

以牛百叶为原料，以PET/AL/PP复合蒸煮袋作包装材料，经原料处理、预煮、油炸、浸汤、真空封口、杀菌等工序，制得牛百叶软罐头。

A complete bovine 18ku-bFGF gene was cloned by nested-PCR and subcloned into expression vector pET-28a.The recombinant plasmid pET-28a-bFGF was transformed into Escherichia coli BL21.At 25℃,recombinant protein was induced by 0.5mmol/L IPTG for 5 hours.Supernatant of cell lysate was purified using Ni-NTA method and the products was submitted to detect the bioactivity by Western-blotting,which indicated that the fusion protein contained recombinant bovine bFGF.Results showed that recombinant bovine bFGF was produced and solubly existed in the supernatant.NIH-3T3 fibroblasts were used to detect the bioactivity of the fusion protein.

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列，并构建了原核表达载体pET-28a-bFGF，将其转化大肠杆菌BL21，在25℃低温条件下，用0.5mmol/L IPTG诱导表达5h，用Ni-NTA亲和纯化细胞裂解上清液，经Western-blotting检测，结果显示，在特定的诱导条件下，重组牛bFGF基因在大肠杆菌中获得了表达，并且主要以可溶性状态存在于细胞中。

The next year I did better still, for I spaded up all the land which I required, about a third of an acre, and I learned from the experience of both years, not being in the least awed by many celebrated works on husbandry, Arthur Young (10) among the rest, that if one would live simply and eat only the crop which he raised, and raise no more than he ate, and not exchange it for an insufficient quantity of more luxurious and expensive things, he would need to cultivate only a few rods of ground, and that it would be cheaper to spade up that than to use oxen to plow it, and to select a fresh spot from time to time than to manure the old, and he could do all his necessary farm work as it were with his left hand at odd hours in the summer; and thus he would not be tied to an ox, or horse, or cow, or pig, as at present.

第二年，我就干得更好了，因为我把总需要量的全部土地统统种上了，只不过一英亩的三分之一，从这两年的经验中，我发现了我没有给那些农业巨著吓倒，包括亚瑟·扬的著作在内。我发现一个人如果要简单地生活，只吃他自己收获的粮食，而且并不耕种得超过他的需要，也不无餍足地交换更奢侈、更昂贵的物品，那末他只要耕几平方杆的地就够了：用铲子比用牛耕又便宜得多；每次可更换一块新地，以免给旧地不断地施肥，而一切农场上的必要劳动，只要他夏天有空闲的时候略略做一做就够了；这样他就不会像日前的人们那样去和一头牛，或马，或母牛，或猪猡，捆绑在一起。

The next year I did better still, for I spaded up all the land which I required, about a third of an acre, and I learned from the experience of both years, not being in the least awed by many celebrated works on husbandry, Arthur Young among the rest, that if one would live simply and eat only the crop which he raised, and raise no more than he ate, and not exchange it for an insufficient quantity of more luxurious and expensive things, he would need to cultivate only a few rods of ground, and that it would be cheaper to spade up that than to use oxen to plow it, and to select a fresh spot from time to time than to manure the old, and he could do all his necessary farm work as it were with his left hand at odd hours in the summer; and thus he would not be tied to an ox, or horse, or cow, or pig, as at present.

扬的著作在内。我发现一个人如果要简单地生活，只吃他自己收获的粮食，而且并不耕种得超过他的需要，也不无餍足地交换更奢侈、更昂贵的物品，那末他只要耕几平方杆的地就够了：用铲子比用牛耕又便宜得多；每次可更换一块新地，以免给旧地不断地施肥，而一切农场上的必要劳动，只要他夏天有空闲的时候略略做一做就够了；这样他就不会像日前的人们那样去和一头牛，或马，或母牛，或猪猡，捆绑在一起。

Mad cow disease, formally known as bovine spongiform encephalopathy, is a degenerative nerve disease in cattle.

疯牛病，是牛棉状脑病的俗称，是发生在牛身上的一种变性疾病。

Bovine spongiform encephalopathy is a fatal neurological disease of adult cattle.

牛海绵状脑病，是一种危害成年牛的致命性神经系统疾病，

A transmissible spongiform encephalopathy of cattle associated with abnormal prion proteins in the brain.

牛海绵状脑病是一种可以在牛中传播的海绵状脑病，与脑组织中异常朊病毒蛋白有关。

Bovine spongiform encephalopathy, widely known as "mad cow disease," is a chronic, degenerative disease affecting the central nervous system of cattle.

牛海绵状脑病，也就是大家都了解的疯牛病，它是一种侵犯牛中枢神经系统的慢性的，变性性疾病。

Earlier this year the discovery of a single cow with bovine spongiform encephalopathy, better known as mad cow disease, crippled the Canadian cattle market.

今年年初，光是一宗牛海绵状脑病的个案，便重创了加拿大的牛只市场。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。