牙骨质

The purpose of this study was to compare the difference between BMSCs co-cultured with cementum and bone, to study the relationship between BMSCs and cementum formation in vitro.

本研究的目的是观察比较BMSCs在牙骨质和骨质表面的钙化情况，研究其与牙骨质生成的关系。

It showed that the matrix of cementum and bone can modulate the behavior of cultured cells.

牙骨质片组和骨片组均有ALP的表达，但牙骨质组较 SS。

Apoptosis of HERS cells may be the signal of cementum development, and some HERS cells may be directly participate in the progress.

HERS细胞的凋亡可能会为成牙骨质细胞分化提供信号，部分HERS细胞可能参与牙骨质的形成。

At 28th day, the cells in cementum group were multilayers and with the mineralized nodules formation.

组织学观察，牙骨质和骨质的表面有新生的类牙骨质。

Cementum is an important mineralized tissue in root formation, however , the precise mechanism of cementum formation remains undetermined.

牙骨质是牙根形成的重要矿化组织，然而，对牙骨质形成机制仍不十分明确。

On the other hand, extract of cemental matrix was separated and clarified from healthy teeth which were extracted because of orthodontic treatment.

从因正畸需拔除的健康牙体表面获得牙骨质基质提取物，分别观察不同作用时间、不同浓度的牙骨质基质提取物对牙龈成纤维细胞和牙周膜成纤维细胞在牙体表面附着的影响。

The pathogenesis of root resorption is still not clear. Cementoblast plays a central role in repair root resorption by synthesizing cementum matrix.

成牙骨质细胞是牙骨质的功能细胞，在牙根形成、牙周病牙骨质的再生以及正畸相关炎性牙根吸收的修复中起着重要的作用。

During the development of dental germ, enamel matrix proteins secreted by epithelial root sheath could induce dental follicle cells to differentiate towards cementoblast and form acellular cementum finally on the surface of tooth root. Many researches of domestic and oversea scholars suggest that enamel matrix proteins could effectively induce periodontal regeneration, and the structure and function of this regenerated tissue are similar to healthy periodontal tissue. Moreover, the regeneration process is nearly the same to that of embryonic development of periodontal tissue.

自发现釉基质蛋白可诱使牙囊细胞向成牙骨质细胞方向分化并在根表面形成无细胞性牙骨质以来，国内外学者做了大量研究，结果表明釉基质蛋白能有效诱导牙周组织的再生，且这种再生组织的结构和功能更接近于健康的牙周组织，并且再生过程也与牙周组织的胚胎发育过程类似。

However, it is not known how orthodontic force influences the function of cementoblast and what is the relationship between the function change of cementoblast with root resorption. So we studied the effect of mechanical stimuli on root resoption by examining the expression OPG and RANKL and exploring the related signal transduction pathway in cementoblast. Methods: Cementoblasts OCCM30 were cultured in DMEM with 10% FBS for 48 hours and starved in DMEM with no FBS for 24 hours, then subjected to mechanical strain by four-point bending system with tension and compression stress at 2000μstrain at 0.5Hz frequency. The flow cytometry was used to examine the cell cycle and proliferation activity after 3h, 6h, 12h, 24h loading respectively. OPG and RANKL mRNA were analysed with real time quantitive RT-PCR after the same loading period as FCM.

本课题利用四点弯曲力学加载系统，采用流式细胞术、实时荧光定量PCR、Western免疫印迹等方法，研究体外培养的成牙骨质细胞株OCCM-30在不同力学环境中增殖活性的变化，以及其表达骨保护素和破骨细胞分化因子的mRNA的变化，探讨应力刺激对成牙骨质细胞表达破骨细胞分化调控蛋白的影响，并通过对应力刺激下成牙骨质细胞分裂原活化蛋白激酶家族中的ERK1/2、P38MAPK活性的变化及其与OPG、RANKL基因表达变化关系的研究，对力学刺激调节破骨细胞分化调控蛋白的相关上游信号转导通路进行初步探讨。

Results: CAP did not affect cell proliferation of HDFC, but increased the ALP activity of HDFC at high protein concentration.

结果：牙骨质附着蛋白对牙囊细胞的增殖无显著影响，但高浓度的牙骨质附着蛋白可增强牙囊细胞碱性磷酸酶活性。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失