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Iron content of the hybrid was significantly and positively correlated with those of their female parents,male parents,high-value of parents,mid-value of parents and difference between high-parents and low-parents,and was significantly positive correlated with low-value of parents.

杂交种籽粒铁含量与母本、父本、高值亲本、中亲和高低值亲本之差的相关均达正向极显著水平,与低值亲本的相关达正向显著水平。

Both selfed seeds and hybrid seeds were obtained from male parent tobacco integrated with Cre gene and female parent tobacco with blocking fragment.

目前,已获得转基因烟草自交种子。以转阻断序列结构为母本,以转Cre基因为父本所进行的杂交组合种子也已经获得。

The cell cultures were monitored by provirus DNA PCR, RT-PCR, reverse transcriptase activity assay and real-time RT-PCR, The results of RT activity and DNA PCR, RT-PCR were positive in the supernatant of cell cultures and viral particles were also clearly observed under electron microscope.

本试验基于代次毒分析结果,选取LTR 变异率较高的U3 区,以驴胎皮肤弱毒疫苗感染性分子克隆株pLGFD3-8 和驴强毒株感染性分子克隆株为父本操作系统进行基因替换,构建EIAV 非编码区强弱毒嵌合的全基因分子克隆,将阳性重组质粒命名为pLGFD9-12。

Up to now, a series of hybridization offsprings were obtained.

测序结果表明,该条带长362bp,在子代与父本中的一致性为100%。

After purification and quantification,1 ugof total RNAs were reverse transcribed and amplified by PCR with5'upstream 10 base random primers and one-base or two-base anchored 3'primer respectively,and the amplified products were displayed on asequencing gel side by side.

本文以父本黄早4,母本U8112和杂种一代掖单4号的玉米幼苗为材料,提取总RNA,用5'端随机引物和3'端单锚定或双锚定引物,进行了mRNA的差异展示研究。

Jiyou768 was selected and bred from the hybridized combination between Line 326 and Line 322.The female parent Line326 possessed Bt toxic protein gene,and the male parent Line 322 possessed sea-island cotton,upland cotton and wild cotton genetic background.

冀优768是利用携带有外源抗棉铃虫Bt基因的326作母本,以具有海岛棉、陆地棉、野生棉遗传基础的322系作父本,其后代在黄枯萎混生重病地、不防治棉铃虫的高压胁迫下,经过连续定向选择育成。

Using the mutant as male parent crossed with Shuihui-202, 9311, D62B, G46B, 527R, respectively. Their seed setting rate all exceeded 30%. Nevertheless, the rate of seed setting by reversed crossing and mixed-pollinating all were zero, and by self-pollinating was less than 1%.

以突变体为父本分别与202R、9311、D62B、527R、G46B杂交,结实率均达30%以上,而反交结实率均为0,几种花粉混合授粉的结实率也为0,突变体的自交结实率低于1%。

Super-sweet corn single-cross variety Kexiangtianyu No.1 was bred by self-line HT01 and self-line HT02.

超甜玉米单交种科湘甜玉1号是以自选系HT01作母本,自选系HT02作父本育成。

The maize inbred line (Chang 453) from Millet Institute, Shanxi Academy of Agricultural Sciences was directly utilized. The high yield large-ear and early-maturing maize single-cross variety Tongdan 36 was bred by inbred-line Chang 453 and Tong 303. It built new heterotic pattern and broke through four heterotic groups limitation now. The heterotic pattern used was Gold-en-queen Х Xianfeng in American (Golden-queen Х P group).

再利用山西省农业科学院谷子研究所玉米自交系长453作母本、同303作父本,育成高产大穗型早熟玉米杂交种同单36号,突破了现有4大血缘的局限,以金皇后血缘材料和美国先锋血缘材料进行杂交,建立了金皇后×P群的新模式。

By removed ovary culture after positive and negative hybridization, produced interspecific hybridization, and have improved the hybrid seed through removed ovary culture and induced to shoot by culture medium MS, and produced hybrid F1, the plant of Brassica junce×B.pekinesis, the advantage characteristic of hybrid plant is similar to female nature;By cytology authenticating, chromosome number of F1 young flower bud cell (2n=28), it is the sum of monoploid chromosome number of female parent Brassica junce(2n=36) and male parent B.pekinesis(2n=20); The hybridization to Brassica junce×B.pekinesis, and embryonic bud come from removed ovary culture, and it found another form of plant embryo saving.

以芸薹属植物芥菜型油菜和白菜为研究对象,对其进行常规正反杂交后子房的离体培养,通过离体子房培养获得的杂种种子经MS培养基诱导成苗,获得了芥菜型油菜与白菜的杂种F1代植株,表现出杂种优势;通过细胞学的方法,鉴定了杂种F1幼蕾体的细胞染色体数为2n=28,为母本芥菜型油菜(2n=36)和父本白菜(2n=20)的单倍体染色体数之和;芥菜型油菜与白菜常规杂交后,经离体子房培养获得了&胚性芽&,确立了植物胚挽救的另一种形式。

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