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熊果酸

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The method appeared to be simple, accurate and can be used to control the quality of Comus Officinalis Sieb. Et Zuec.

HPLC法可使熊果酸和齐墩果酸达到基线分离,简便准确,可用于药材质量监控。

A method for the determination of the total amount of ursolic acid and oleanolic acid in Cornus officinalis and its steeping wine was developed.

建立了测定山茱萸及其浸泡酒中熊果酸和齐墩果酸总量的方法。

Ursolic acid, which has immunity enhancement in the Cornus, was chosen as primary index in this research. The method of reversed-phase high performance liquid chromatography with ultraviolet detector was developed to determine the content of ursolic acid. The L9(34) orthogonal experimental design was used for Cornus to further process and explore its health product using the content of ursolic acid and the rate of paste as the assessment indexes.

本研究以山茱萸中具有增强免疫功能的熊果酸为主要考察指标,采用反相高效液相色谱-紫外检测法作为熊果酸含量的测定方法,以出膏率为参考指标,采用L9(34)设计正交试验,对山茱萸药材进行了深加工,开发其保健品;对所得到的提取物中的多糖进行了定性鉴定。

Methods: The effects of pressure, temperature, time, CO2 flow rate types, and volume of entrainer on the urosolic acid extracts were studied. The optium conditions for SFE CO2 was determined.

探讨萃取压力、萃取温度、萃取时间、夹带剂类型及用量对熊果酸收率的影响,确定了超临界CO2萃取车前草中熊果酸的适宜条件。

Methods The effects of pressure, temperature, time, CO2 flow rate types, and volume of entrainer on the urosolic acid extracts were studied. The optium conditions for SFE CO2 was determined. The technology of SFE CO2 was compared with that of traditional solvent extraction.

探讨萃取压力、萃取温度、萃取时间、CO2流量、夹带剂类型及用量对熊果酸收率的影响,确定了超临界CO2萃取山茱萸中熊果酸的适宜条件,并将超临界CO2萃取法与传统的有机溶剂提取法进行了比较。

HPLC method was proved to be sensitive, fast and accurate. It can be used for the determination of ursolic acid and oleanolic acid in different colour Rhododendron simsii Planch flowers.

本方法灵敏、准确,重现性好,可用于不同花色映山红花中熊果酸和齐墩果酸含量的测定。

Results Ten known constituents were isolated and their structures were identified as 23-hydroxyursoIic acid (1), alphitolic acid (2), ursolic acid (3), dihydro-β-cyclopyrethrosin (4), sesamin (5),β-sitosterol (6), clauslactone-K (7), betulinic acid (8), lingueresinol (9), ent-isolariciresinol (10), respectively.

结果:从上述三种植物中共分离鉴定了10个化合物,其分别是:23-羟基熊果酸(1)、alphitolic acid(2)、熊果酸(3)、二氢-B-环除虫菊精(4)、芝麻素(5)、β-谷甾醇(6)、clauslactone-K(7)、白桦脂酸(8)、lingueresinol(9)和ent-isolariciresinol(10)。

On the basis of spectral analysis, these compounds were identified as Kaempferol , Kaempferol-3-O-β-D-glucopyranoside , Kaempferol-3-O-β-D-galactopyranoside, Quercetin , Quercetin-3-O-β-D-arabinopyranoside, Quercetin-3-O-β-D-galactopyranoside, gallic acid, methyl gallate-4-O-β-D- galactopyranoside, caffeicacid, methyl caffeate, 4-cafeoyl-(3"a, 4"a, 5"β)- trihydroxyquinic acid, 3"-cafeoyl-(3"a, 4"P, 5"P )-trihydroxyquinic acid methyl ester, 5"-cafeoyl-galactonic acid , pumiloside, loganic acid , 5R,6S,7E-megastima-3-on-7-en-9-ol 9-O-β-D-glucopyranoside. 2a, 3β-dihydroxy-oleanoic acid , 2a, 3β, 23-trihydroxy-oleanoic acid, 3a, 6a-dihydroxy-oleanoic acid, 3β-hydroxy-ursoic acid, 2a, 3a, 23-trihydroxy-ursoic acid , 2a, 3β, 23-trihydroxy-ursoic acid, 2β, 3β, 19a -trihydroxy-ursoic acid, 2a , 3β, 19a -trihydroxy-ursoic acid-28-O-β-D-glucopyranoside , 2a. 3a, 19a-trihydroxy-ursoic acid-28-O-β-D-glucopyranoside, 2a, 3a, 19a, 23-tetrahydroxy-ursoic acid, 1a, 3a, 19a, 23-tetrahydroxy-ursoic acid-28-O-β-D-glucopyranoside , 3a, 19a, 23-trihydroxy-ursoic acid.

从珙桐中分离鉴定得到了28种化合物,它们分别是:山奈酚,3-O-β-D-吡喃葡萄糖基-山奈酚甙,3-O-β-D-吡喃半乳糖基-山奈酚甙,槲皮素,3-O-β-D-吡喃阿拉伯糖基-槲皮素甙,3-O-β-D-吡喃半乳糖基-槲皮素甙,没食子酸,4-O-β-D-吡喃半乳糖基-没食子酸甲酯,咖啡酸,咖啡酸甲酯,4-咖啡酰基-(3&α,4&α,5&β)-三羟基鸡纳酸,4-咖啡酰基-(3&α,4&β,5&β)-三羟基鸡纳酸甲酯,5'-咖啡酰基-半乳糖酸,pumiloside,loganic acid,5R,6S,7E-megastima-3-on-7-en-9-ol9-O-β-D-glucopyranoside,2α,3β-二羟基齐墩果酸,2α,3β,23-三羟基齐墩果酸,3α,6α-二羟基齐墩果酸,3β-羟基熊果酸,2α,3α,23-三羟基熊果酸,2α,3β,23-三羟基熊果酸,2β,3β,19α-三羟基熊果酸,2β,3β,19α-三羟基熊果酸-28-O-β-D-吡喃葡萄糖甙,2α,3α,19α-三羟基熊果酸-28-O-β-D-吡喃葡萄糖基甙,2α,3α,19α,23-四羟基熊果酸,2α,3α,19α,23-四羟基熊果酸-28-O-β-D-吡喃葡萄糖基甙,3α,19α,23-三羟基熊果酸

The content of ursolic acid is determined with HPLC method.

结果枇杷叶中熊果酸的含量为0.9%以上,不同方法处理后的药渣中熊果酸均有不同程度的残留。

Methods Urolio acid was from Fructus Corni with refluent ethanol extraction and SFE CO2,with urolio acid as the index.

方法分别采用超临界CO2法、乙醇回流法提取山茱萸中的熊果酸,以熊果酸作检测指标进行定量分析。

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