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烯醇化

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After 48 hours pre-induction, the percentage of nestin was 24%, which was 4.6% in the control group. After 48 hours induction, BMSCs exhibited the typical form of perikaryon with pyknotic cell body and prominence projected like that of neuron.

诱导48 h后,巢蛋白染色呈阳性,并可见大量神经元特异性烯醇化酶和胶质纤维酸性蛋白阳性细胞出现;对照组均呈阴性表达。

After 48 hours induction, BMSCs exhibited the typical form of perikaryon with pyknotic cell body and prominence projected like that of neuron. The cells were positively stained with NSE and GFAP in the control group.

诱导48 h后,巢蛋白染色呈阳性,并可见大量神经元特异性烯醇化酶和胶质纤维酸性蛋白阳性细胞出现;对照组均呈阴性表达。

Methods The activity was determined by 3 - phosphoglycerate as substrate, the enolase and a series of coupling to 340 nm wave-length automatic analyzer in the determination of total activity could be derived.

PGAM活性测定采用3-磷酸甘油酸为底物,经烯醇化酶等一系列偶联,以340 nm波长在自动分析仪上测定,根据NADH吸光度变化可求出PGAM总活性。

Objective: To explore the change and significance of serous S100B and NSE in patients with acute cerebrovascular disease.

目的:探讨急性脑血管病患者血清S100B和神经元特异性烯醇化酶的变化及其临床意义。

BMSCs expressing Ad-GFP had three types of subpopulation structures under a fluorescence microscope; when the virus titer was ≥ 1×10^6 PFU/mL, BMSCs proliferation was suppressed at the early stage, in a dose-dependent manner.

荧光显微镜下可见表达Ad—GFP的BMSCs有三种亚群结构:滴度≥1 10^6PFU/mL时,BMSCs增殖在早期受到抑制,且呈剂量依赖;转染Ad—GFP的BMSCs经β一巯基乙醇诱导可分化为神经元样细胞,神经元特异性烯醇化酶阳性。

Objective To explore the changes and clinical significance of neuropeptide Y and neuron-specific enolase levels in children with convulsibility diseases.

目的 探讨神经肽Y和神经元特异性烯醇化酶在小儿惊厥性疾病中的水平变化及其临床意义。

Results ADSCs, transfected by lentivirus, can differentiate into neuron-like cells and express NSE in cytolymph.

结果 慢病毒转染ADSCs形态类似神经元,胞浆神经元特异性烯醇化酶表达阳性。

Results ADSCs, transfected by lentivirus, can differentiate into neuronlike cells and express NSE in cytolymph.

结果 慢病毒转染ADSCs形态类似神经元,胞浆神经元特异性烯醇化酶表达阳性。

The second synthetic strategy commenced from butane-1,4-diol 50, which underwent monobenzylation, Swern oxidation, enolization followed by acetylation and debenzylation to yield an intermediate 46 in an overall yield of 54% in a 4-step procedure.

第二条路线从1,4-丁二醇50开始,这个化合物经过单苄基化、Swern氧化、烯醇化随后乙酰化和脱苄基化共4步反应得到中间体46,总产率54%。

They readly enolize in alkaline soluions to reduce ions such as cu2+ and fe63-. therefore, they are called "reducing sugars".

它们在碱性溶液中易于烯醇化而使cu2+、fe63+之类的离子还原,因此称它们为&还原糖&。

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