热变性

The effect of heating treatment of the substrate on hydrolysis effect before hydrolysis was also investigated.

另外还研究了酶解之前牡蛎肉的热变性处理对水解效果的影响。

The molecular weight of broad bean vicilin and legumin measured by gel filtration chromatography were 135000 and 351000, DSC showed that their denature temperatures were 72℃ and 87℃ respectively. The legumin had a lowest solubility at pH 4. 5-5. 0 whereas the vicilin at pH5. 5. Vicilin and legumin were all possessed low levels of α-helical and high β-sheet secondary structure fractions. The total content of α-helical,β-sheet and β-turn fraction of vicilin and legumin reached to 90% and 78% separately showed that they had a high ordered structure in solution.

对两种组分的凝胶层析表明7S和11S的相对分子质量分别为135000和351000；DSC分析显示其热变性温度分别为72℃和87℃；溶解度分析表明：11S球蛋白在pH4.5-5.0之间，7S球蛋白在pH5.5时具有最低的溶解度；圆二色性分析结果显示7S和11S球蛋白的β-折叠结构含量分别高达40.5％和38.1％，证明二者都属于β-折叠型蛋白质，二者的α-螺旋、β-折叠和β-转角结构的含量总和分别达到了90％和78％，从而说明它们在溶液中的结构是高度有序的。

Triose phosphate isomerase was prepared and purified from chicken breast muscle. The equilibrium unfolding of TIM induced by guanidine hydrochloride was investigated by following the changes in intrinsic fluorescence, circular dichroism and second-derivative spectroscopy.

从鸡胸肌中纯化出磷酸丙糖异构酶(triose phosphate isomerase， TIM)，通过蛋白质内源荧光，圆二色性，紫外吸收二阶导数光谱等多种研究溶液构象的方法，对TIM被盐酸胍和热变性过程进行了详细的研究。

The interaction of S-metolachlor with calf thymus DNA was investigated using UV absorption spectra, fluorescence spectra, thermal denaturation and viscometry of DNA.

应用紫外光谱、荧光光谱、DNA热变性法以及粘度法研究了S-异丙甲草胺与小牛胸腺DNA的相互作用。

The variation of the molecular motions of lysozyme and water in the process of hydration and tnermodcnaturation has been discussed by the changes of line-width parameter with hydration and temperature.

用线宽参量随水合度及温度的变化讨论溶菌酶在水合及热变性过程中溶菌酶分子及水分子运动性的变化。

Ferritin from the liver of a salt water fish, Dasyatis akajei, herein called DALF, was isolated and purified by thermal denaturation, ammonium sulphate fractionation and ammonium reverse extraction, Sephacryl S-300 gel filtration and DEAE- cellulose anion exchange chromatography.

通过热变性处理、硫酸铵分级沉淀、排阻层析和离子交换层析等技术，分离纯化了魟鱼肝铁蛋白Liver ferritin of Dasyatis akaiei。

Thermally denatured DNA was preconcentrated at pretreated GCE by its adsorption in open circuit for 5min or at the potential of+0.3V for 90s, and produced two well-defined oxidation peaks of guanine and adenine residues at+0.80V and+1. 11V in pH 5. 0 phospoate buffer, respectively, but the native DNA did not produce any peaks at the same conditions.

在pH 5.0的磷酸盐缓冲液中，于+0.3V富集90秒或在开路条件下吸附富集5分钟，热变性DNA可以吸附富集在预处理玻碳电极表面，阳极扫描时，鸟嘌呤和腺嘌呤残基产生两个很好的氧化峰，峰电位分别为+0.80V和+1.11V，而在同样条件下，天然DNA却几乎不出现峰。

Control run were taken under similar conditions replacing active enzyme by same volume of heat denatured enzyme preparation.

控制运行采取了类似条件下取代积极的酶由同一卷的热变性酶制剂。

A convenient quantitation of low level of thermally denatured DNA was proposed.

提出了一种定量测定热变性DNA的简便方法。

The thermal denaturation was approximately 60℃.15strains of deodorizing bacterium were isolated in advantage of the solution ofmanure.

结果表明，FH3适宜的酶反应条件为温度在45～55℃，pH在4.5～5.5之间，FH3的酶热变性温度在60℃左右。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。