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热变性

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The adduct compositions of DNR with fish sperm DNA and thermally denaturated DNA were determined.

并对柔红霉素与鱼精DNA和热变性DNA复合物的组成及复合物的形成常数作了测定。

Glucose, glycerol, sucrose, mannitol have good protection from the heat denaturalization of deer placenta polypeptide and the impact of glucose is the best, the 20 % concentration of the protector is appropriate.

葡萄糖、丙三醇、蔗糖、甘露醇对鹿胎盘多肽的热变性有明显的保护作用,其中以葡萄糖的保护效果最好,保护剂的浓度为20%时较为适宜。

The excessive amounts of NaCl in solution act as the denaturant in the thermal denaturation of lysozyme.

当NaCl浓度高于0.6 mol/L时,第二次热变性时的溶菌酶完全失去活性。

It is also found that melting temperature curve shift in the result of Thermal Denaturation analysis.

由DNA热变性曲线分析结果,证实有键结而造成曲线位移的现象。

The amino acid composition and circular dichroism of two fish trypsin isozymes were determined.

DSC表明酶的热变性温度分别为66.3℃和67.3℃。

The equilibrium association constant K of DNR with fish sperm DNA is 1.98×105 mol/L and that of DNR with denaturated DNA is 2.29×104 L/mol.

研究发现嵌入作用是柔红霉素和天然DNA之间的主要作用方式;并且柔红霉素和天然DNA之间的作用要强于和热变性单链DNA之间的作用。

Solubility and amino acid composition of rice protein s from rice flour and rice dreg were analyzed.

为探讨热变性米蛋白的性质与结构关系,分析了大米蛋白加热前后的溶解性能和氨基酸组成变化。

Using the flexibility of proteins, egg white albumin was pore-formed and heat-denatured in the presence of substrate template. BSA protein and herring sperm DNA were trasted by precipitation method in organic solvent.

利用蛋白质在水溶液中的柔性,分别采用热变性法对蛋清蛋白质进行生物印迹和沉淀法对BSA进行生物印迹,并在BSA印迹的基础上又印迹了鲱鱼精脱氧核糖核酸。

In the preparation of bio-imprinted enzymes, two bio-imprinting approaches are available:(1) bio-imprinting of egg albumin using heat-denaturalization; and (2) bio-imprinting of egg albumin using bovine serum albumin using dissolving-precipitation process.

在制备生物印迹酶方面,利用蛋白质在水溶液中的柔性,分别采用两种方法对蛋白质进行生物印迹:以热变性法对蛋清蛋白进行生物印迹,以及用溶解沉淀法对牛血清蛋白进行生物印迹。

Except for unstable V66W102, the guanidine and thermal denaturation of fragments are cooperative and well approximated by the two-state transition.

除了不稳定的V66W102,其它片段的盐酸胍和热变性过程协同,符合二态模型。

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