热变性

The group of low migration nucleases were prepared by ammonium carbonate processing +acid denaturation + thermal denaturation + acetone extraction.

在提取过程中产生两组核酸酶，在SDS-PAGE-DNA功能胶中迁移率较慢的一组采用碳酸铵处理+酸变性+热变性+丙酮沉淀的提取方法来获得较好的层析样品。

The thermodynamic parameters of thermal and chemical denaturation were determined for bIgG using both methods.

DSC和荧光光谱实验结果表明， bIgG的热变性和热化学变性过程都是较复杂的不可逆过程，这个过程可被看作一个三态变构过程。

Therefore, both chemical denaturation methods can yield different structurally unfolded states of the bIgG.

实验结果还表明单纯的热变性只能导致bIgG的不完全变性，而即使是在高浓度变性剂存在时的bIgG热化学变性，尿素和盐酸胍分别导致的bIgG热化学变性的去折叠态也是不同的。

This paper discusses the effect of the thermal denaturation on the structure,functions and enzymatic hydrolysis of proteins.

本文论述了热变性对蛋白质结构、功能性质以及酶解性质的影响，揭示了蛋白质热变性后理化性质的变化是其二级、三级或四级结构改变的结果，并讨论了其它因素对热变性的影响。

Proteolysis and denaturation of porcine protein s on heating were investigated.

在加热的条件下，猪肉蛋白质发生热变性和热降解。

The result showed that the temperature of denature for the formeris 106.67°C, while that for the latter is 66.84°C. These results showed that theoligosaccharide chain of SPWSG played a role of strengthening resistance todenaturation.

采用DSC对甘薯水溶性糖蛋白的热力学性质进行了研究，结果表明甘薯水溶性糖蛋白的热变性温度为106.67°C，而去糖基后的热变性温度为66.84°C，说明糖蛋白上的寡糖链对其有较好的稳定作用，具有增强糖蛋白抗变性的功能。

Objective To establish a simple,accurate and practi cal method of extracting plasmid from gram-negative and gram-positive bacteria ,Method we selected,Biswajit sahas method and comported others method, such as controlling pH andtemperature strictly and increasing EDTA-Na2 capacity.

为建立一种革兰氏阴性及阳性菌均能分析的质粒提取方法，以Biswajit saha的方法为基础，对比酸酚法、热变性法、碱变性法、酶法等方法，研制符合国内一般实验室的方法。

The screening was carried out by single strand conformational polymorphorsim : the PCR product precipitation was dissolved in 20μl denaturing and loading buffer (95%+20mMEDTA+ 0.05% bromophenol blue+0.05% xylene cyanol), heat-denatured in 95 ℃ to two single DNA strands.

通过单链构象多态性分析筛查RDS基因突变；沉淀回收的PCR产物以20μl变性上样液（95%甲酰胺+20mMEDTA+0.05%溴酚蓝+0.05%二甲苯蓝）溶解后热变性为两条DNA单链后，上样于8%非变性聚丙烯酰胺凝胶（含5%甘油），700伏电压、4℃电泳16小时。

Heteroduplex molecules have been made by mixing pJSA1175 and pJSAl175ADRV4, pJSA1175ADRV4 and pJSAl175RRV4 DNA molecules which have been linearized by Pst Ⅰ and Bgl Ⅱ respectively, denaturing them in alkaline or high temperature, allowing them to renature and then spreading in the presence of formamide.

以痘苗病毒表达载体pJSA1175及重组质粒pJSA1175ADRV4(含ADRV VP4基因)、pJSA1175RRV4(含猴轮状病毒VP4基因)为材料，用碱变性方法或热变性方法制备了载体质粒与重组质粒DNA之间的异源双链，电镜下成功观察到载体序列部分经变性、复性后配对呈双链状态，而外源基因区域突出呈单链环状结构，测量长度为2.3kb。

A study on thermal denaturation of hen egg-white lysozyme was carried out by differential scanning calorimetry and modulated temperature differential scanning calorimetry. It was found that DSC profiles of HEWL were dependent on heating rates and concentrations of enzyme as well as cosolvent.

利用差示扫描量热和温度调制差示扫描量热研究了鸡蛋白溶菌酶在纯水及二甲基亚砜/水混合溶剂中的热变性过程，探讨了酶的浓度、扫描速率和共溶剂的含量对热变性行为的影响规律。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。