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The results showed that expression plasmid pET22b-lysB was constructed successfully. Highly purified recombination protein was obtained 33.2 mg from 1 L LB culture medium. A screening for His-LysB activity on esterase and lipase substrates confirmed the lipolytic activity. With p-nitrophenyl butyrate as substrate, the thermal stability of the enzyme was poor when the temperature was above 30oC. The enzyme exhibited higher stability at pH 5.0–9.5. The optimum temperature and pH for the lipolytic activity of His-LysB were 23oC and 7.5 respectively. Under the optimum conditions, the specific activity of His-LysB was 1.3 U/mg. Zn2+, Cu2+, Mg2+, Mn2+and phenylmethane sulfonyl fruoride severely inhibited the lipolytic activity of His-LysB.

结果表明:成功构建了pET22b-lysB表达载体,并从1 L的LB培养物中获得了33.2 mg高纯度重组蛋白;His-LysB具有分解脂肪的能力,属于脂肪酶;生物化学特性分析表明:丁酸对硝基苯为水解底物,His-Lys热稳定性不佳,30℃以下比较稳定,随着温度的升高,稳定性逐渐降低;该蛋白具有较高的pH值适应性,pH 5.0~9.5范围内稳定性较高;在23℃和pH 7.5时酶活力最高,其比酶活为1.3 U/mg;金属离子Zn2+、Cu2+、Mg2+、Mn2+和苯甲基磺酰氟抑制剂对酶活具有强烈的抑制作用。

The macrographic investigation and the analyses on chemical composition, metallography and mechanical property at normal temperature of the damaged tube material of thermal recovery steam-injection boiler used for heavy oil recovery were carried out.

对稠油开采设备热采注汽锅炉破损炉管的材料进行了宏观检查、化学成分分析、金相分析、常温力学性能分析,并检测了给水水质,探讨了锅炉炉管在当前条件下的腐蚀机理。

Flow patterns,spatial distribution of temperature and chemical species,and growth rates during growth processes of GaN in planetary metalorganic chemical vapor deposition reactor are simulated three-dimensionally based on computational fluid dynamics theory.

基于计算流体力学在三维空间中模拟了水平行星式金属有机物化学气相沉积反应器生长GaN材料的流场、热场、反应物与生成物的分布以及材料生长速率等重要物理参数。

The basic monomeric unit should possess chemical and thermal stability and, upon polymerization, provide a product with unique properties.

单体单位应具备的基本化学和热稳定性,一经聚合,提供一个独特的产品性能。

The effects of high-energy ball milling including the fining and mechanochemical on the carbothermal reduction nitridation of alumina were investigated.

研究了高能球磨氧化铝的细化和机械力化学作用及球磨对碳热还原反应合成氮化铝的影响。

The main contents are: Status and prospects of micro/nano fabrication technology, semiconductor substrates, vacuum science and plasmas. Several unit processes of microfabrication like diffusion, thermal oxidation, ion implantation, optical and nonoptical lithography, etching, physical deposition, chemical vapor deposition and epitaxial growth. Process integration of these unit processes for example CMOS technique and silicon bipolar technique, microelectro mechanical systems and some nano fabrication technologies will be introduced.

主要内容有:微米/纳米制造技术的现状和展望、半导体衬底、真空科学和等离子体,各种单项微电子加工工艺,如扩散、热氧化、离子注入、光学和非光学光刻、刻蚀、物理淀积、化学气相淀积、外延生长等,以及各种单项工艺的集成技术,如CMOS技术和硅双极型工艺技术,并初步介绍微电子机械系统和纳米加工技术。

The effects of solid content of latex, reaction temperature, the amount of phenylhydrazine and pepegal O on the viscosity-average relative molecular mass M(subscript n of LNR were investigated, and the optimization of these influence factors was conducted by orthogonal test.

以天然橡胶浓缩胶乳为原料,在热氧条件下采用苯肼为催化剂通过化学降解法制备液体天然橡胶,考察了胶乳的总固物质量分数、反应温度、稳定剂平平加O和苯肼用量对LNR黏均相对分子质量M(下标 n的影响,并通过正交实验进行优化。

X-ray diffraction,transmission electron microscopy, BET surface area measurement and thermal analysishave been used to determine the characteristics of the nanopowdered silver prepared by chemical reduction with the polymolecular protectant.

用X -射线衍射、透射电镜、比表面测定和热分析技术,对从高分子保护化学还原法制得的纳米Ag粉,做了相关的测定表征;从表观比热的测定结果探讨这一亚稳微晶体系在加热过程和热处理后所发生的各种变化。

Polytropic heat was introduced in the model to guarantee the bubble's growth under the action of positive chemical potential difference between two phases.

该模型中引入多变热,以保证汽泡在正化学势差作用下长大。

The rheological property of a bisphenol A cyanate resin was investigated by means of parallel plate method, it was modified by isothermal precure process. The effects of precure process on the chemical structure of resin were studied by IR, DSC and GPC methods.

本文采用平板法研究了一种双酚A型氰酸酯树脂的流变特性,通过恒温预固化的方法对其进行改性,并借助红外光谱、差示扫描量热法和凝胶渗透色谱法等手段分析了预固化对氰酸酯树脂化学结构的影响。

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I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时,滚道或滚动元件褪色是很常见的特征。

The lawyer's case blew up because he had no proof.

律师的辩护失败,因为他没有证据。